{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T14:25:27Z","timestamp":1788963927271,"version":"build-2803163510"},"reference-count":51,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND:<\/jats:title>\n                    <jats:p>In addition to their fundamental roles in preserving vascular integrity, platelets also contribute to tumor angiogenesis and metastasis. However, despite being a reservoir for angiogenic and metastatic cytokines, platelets also harbor negative regulators of tumor progression. Angpt1 (angiopoietin-1) is a cytokine essential for developmental angiogenesis that also protects against tumor cell metastasis through an undefined mechanism. Although activated platelets release Angpt1 from \u03b1-granules into circulation, the contributions of platelet Angpt1 to tumor growth, angiogenesis, and metastasis have not been investigated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>\n                      Using cytokine arrays and ELISAs, we first compared platelet Angpt1 levels in breast and melanoma mouse tumor models to tumor-free controls. We then assessed tumor growth and metastasis in mice lacking megakaryocyte and platelet Angpt1 (Angpt1\n                      <jats:sup>Plt KO<\/jats:sup>\n                      ). The spontaneous metastasis of mammary-injected tumor cells to the lungs was quantified using RT-PCR (reverse transcription-polymerase chain reaction). The lung colonization of intravenously injected tumor cells and tumor cell extravasation were determined using fluorescent microscopy and flow cytometry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>\n                      Platelet Angpt1 is selectively upregulated in the PyMT (polyoma middle tumor antigen) breast cancer mouse model, and platelets are the principal source of Angpt1 in blood circulation. While primary tumor growth and angiogenesis were unaffected, Angpt1\n                      <jats:sup>Plt KO<\/jats:sup>\n                      mice had both increased spontaneous lung metastasis and tumor cell lung colonization following mammary or intravenous injection, respectively. Although platelet Angpt1 did not affect initial tumor cell entrapment in the lungs, Angpt1\n                      <jats:sup>Plt KO<\/jats:sup>\n                      mice had increased tumor cell retention and extravasation. Serum from Angpt1\n                      <jats:sup>Plt KO<\/jats:sup>\n                      mice increased endothelial permeability and reduced VE (vascular endothelial)-cadherin expression at endothelial junctions compared with serum from control mice (Angpt1\n                      <jats:sup>WT<\/jats:sup>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS:<\/jats:title>\n                    <jats:p>Platelets provide an intravascular source of Angpt1 that restrains tumor metastasis by preserving the lung microvasculature to limit tumor cell extravasation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/atvbaha.124.321189","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T05:01:33Z","timestamp":1721883693000},"page":"2024-2037","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":8,"title":["Platelet Angiopoietin-1 Protects Against Murine Models of Tumor Metastasis"],"prefix":"10.1161","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1100-8409","authenticated-orcid":true,"given":"Harvey G.","family":"Roweth","sequence":"first","affiliation":[{"name":"Division of Hematology, Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA (H.G.R., M.W.M., E.M.C., S.A.M., P.L.K., E.M.B.)."},{"name":"Harvard Medical School, Boston, MA (H.G.R., I.C.B., P.L.K., I.A., A.A.S., E.M.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle C.","family":"Becker","sequence":"additional","affiliation":[{"name":"Harvard Medical School, Boston, MA (H.G.R., I.C.B., P.L.K., I.A., A.A.S., E.M.B.)."},{"name":"Vascular Biology Program, Department of Surgery, Boston Children\u2019s Hospital, MA (I.C.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7494-0468","authenticated-orcid":true,"given":"Michael W.","family":"Malloy","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA (H.G.R., M.W.M., E.M.C., S.A.M., P.L.K., E.M.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily M.","family":"Clarke","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA (H.G.R., M.W.M., E.M.C., S.A.M., P.L.K., E.M.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie A.","family":"Munn","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA (H.G.R., M.W.M., E.M.C., S.A.M., P.L.K., E.M.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priya L.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Division of Hematology, Department of Medicine, Brigham and Women\u2019s Hospital, Boston, MA (H.G.R., M.W.M., E.M.C., S.A.M., P.L.K., E.M.B.)."},{"name":"Harvard Medical School, Boston, MA (H.G.R., I.C.B., P.L.K., I.A., A.A.S., E.M.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0320-2735","authenticated-orcid":true,"given":"Ivan","family":"Aivasovsky","sequence":"additional","affiliation":[{"name":"Harvard Medical School, Boston, MA (H.G.R., I.C.B., P.L.K., I.A., A.A.S., E.M.B.)."},{"name":"Division of Cardiovascular Medicine, Beth Israel Deaconess Medical Center, Boston, MA (I.A., K.T., 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