{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T18:16:52Z","timestamp":1778177812378,"version":"3.51.4"},"reference-count":58,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND:<\/jats:title>\n                    <jats:p>Dysregulated proteolysis is implicated in thoracic (thoracic aortic aneurysm [TAA]) and abdominal aortic aneurysm (AAA) pathogenesis, but proteolytic landscapes (degradomes) of aneurysmal and normal aorta and contributions of individual proteases remain undefined. Here, a proteome-wide approach was used to define and compare TAA and AAA degradomes and uncover the specific role in aortic remodeling of 2 proteases consistently identified in the aneurysms, CMA1 (mast cell chymase) and MMP9 (matrix metalloprotease 9).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>The mass spectrometry-based N-terminomics strategy, terminal amine isotopic labeling of substrates, was applied to Marfan syndrome TAAs (n=5), AAAs (n=16), and nondiseased thoracic aorta (n=4), and abdominal aorta (n=4) in a forward degradomics application, that is, to define substrate and protease degradomes. 8-plex iTRAQ terminal amine isotopic labeling of substrates was used for quantitative comparison of the tissue cohorts. Cleavage sites of CMA1 and MMP9 were sought by reverse degradomics, that is, digestion of aortic proteins with these proteases, followed by terminal amine isotopic labeling of substrates. CMA1 and MMP9 proteolysis of biglycan was further resolved using amino-terminal oriented mass spectrometry of substrates.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>We experimentally annotated 20\u2009885 proteolytically derived peptides and identified 129 proteases in the aortic tissues. Quantitative substrate degradome comparisons identified specific differentially modulated pathways and networks in TAAs and AAAs. Reverse degradomics elucidated &gt;300 CMA1 and MMP9 substrate cleavage sites, of which many, including orthogonally validated biglycan cleavages, occurred in the disease degradomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS:<\/jats:title>\n                    <jats:p>Unbiased forward degradomics of the aortic wall from TAA, AAA, and nondiseased tissue provides a systems biology view of aortic wall breakdown and a new resource for its hitherto occult proteolytic landscape, demonstrating widespread extracellular matrix remodeling with disproportionate impact on proteoglycans. The findings provided insights into aortic aneurysm pathways and disease biomarkers and suggest involvement of numerous proteases. Mapping of specific proteolytic contributions of CMA1 and MMP9 illustrates a strategy for defining the activities of all proteases involved in aortic disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/atvbaha.125.323810","type":"journal-article","created":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T09:00:23Z","timestamp":1775120423000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Forward and Reverse Degradomics of Aortic Aneurysms Uncovers Their Proteolytic Landscapes and the Roles of MMP9 and Mast Cell Chymase"],"prefix":"10.1161","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5274-5122","authenticated-orcid":true,"given":"Sumit","family":"Bhutada","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering (S.B., D.R.M., S.S.A.), Cleveland Clinic Research, Cleveland, OH."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3517-3716","authenticated-orcid":true,"given":"Daniel R.","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering (S.B., D.R.M., S.S.A.), Cleveland Clinic Research, Cleveland, OH."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1427-7583","authenticated-orcid":true,"given":"Frank","family":"Cikach","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery, Cleveland Clinic Heart and Vascular Institute, OH (F.C., E.G.S., E.H.B., E.E.R.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0786-179X","authenticated-orcid":true,"given":"Emidio","family":"Germano da Silva","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery, Cleveland Clinic Heart and Vascular Institute, OH (F.C., E.G.S., E.H.B., E.E.R.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6884-6369","authenticated-orcid":true,"given":"Belinda B.","family":"Willard","sequence":"additional","affiliation":[{"name":"Proteomics and Metabolomics Core, Cleveland Clinic Research, OH (B.B.W.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9087-0995","authenticated-orcid":true,"given":"Bhama","family":"Ramkhelawon","sequence":"additional","affiliation":[{"name":"Division of Vascular and Endovascular Surgery, Department of Surgery, New York University Langone Medical Center (B.R.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7835-6045","authenticated-orcid":true,"given":"Mina K.","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular and Metabolic Sciences (M.K.C.), Cleveland Clinic Research, Cleveland, OH."},{"name":"Department of Cardiovascular Medicine, Cleveland Clinic Heart, Vascular and Thoracic Institute, OH (M.K.C.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5947-8282","authenticated-orcid":true,"given":"Shataakshi","family":"Dahal","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Lehigh University, Bethlehem, PA (S.D., A.R.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9899-9283","authenticated-orcid":true,"given":"Anand","family":"Ramamurthi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Lehigh University, Bethlehem, PA (S.D., A.R.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2403-8268","authenticated-orcid":true,"given":"John","family":"Barnard","sequence":"additional","affiliation":[{"name":"Department of Quantitative Health Sciences (J.B.), 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