{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T06:52:00Z","timestamp":1772952720512,"version":"3.50.1"},"reference-count":54,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","license":[{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND:<\/jats:title>\n                    <jats:p>The venous and lymphatic outflows from organs may contain molecular signatures related to tissue function, but deep biomolecular analyses that compare outflow composition are lacking. Here, using blood and lymph samples from a piglet model system and mass spectrometry analysis, we compare protein and lipid cargo from 9 venous and 3 lymph depots centered on intestinal outflow.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>We obtained venous blood and plasma from piglets using new methods for dissection and sample collection. We applied mass spectrometry\u2013based proteomics and lipidomics, using both a low-volume method and an additional proteomics sample preparation method, the Seer Proteograph XT high coverage method for the proteomics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>We detected 622 proteins and 1315 lipids across lymph and plasma. With the additional Seer proteomics method, we detected a further 7771 proteins across a subset of samples. We observed both expected and novel enrichments of proteins, including CCL21 (C-C motif chemokine ligand 21) and IGFBP (insulin-like growth factor-binding protein) 7 as proteins strongly enriched in lymph. When comparing lymph depots, we found that thoracic duct lymph is distinct from lymph draining the proximal and distal small intestine, especially in their lipidomic profiles, possibly reflecting differences in dietary lipid uptake. By performing integrative multiomics of proteomics and lipidomics, we show that apoproteins, such as the related apoA1 (apoprotein A1) and apoA2 (apoprotein A2) proteins, correlate with different lipid profiles and may associate with distinct functions across the plasma depots.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS:<\/jats:title>\n                    <jats:p>These data identify molecules and biomarkers selectively enriched in adjacent lymph and venous drainage depots from the gastrointestinal tract. The analyses and figures present in this work are expanded upon in an interactive companion Web application at gutveinlymphomics.com, facilitating access to our integrated multiomics and advancing understanding of biomolecular trends across the intestinal tract.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/atvbaha.125.323883","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T10:00:47Z","timestamp":1769076047000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Proteomic and Lipidomic Atlas of Gut-Associated Lymph and Venous Depots in Female Piglets"],"prefix":"10.1161","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6156-342X","authenticated-orcid":true,"given":"Benton J.","family":"Anderson","sequence":"first","affiliation":[{"name":"Department of Biomolecular Chemistry (B.J.A., C.E.M., J.J.C., K.A.O.), University of Wisconsin-Madison."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0568-0415","authenticated-orcid":true,"given":"Corinne E.","family":"Moss","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Chemistry (B.J.A., C.E.M., J.J.C., K.A.O.), University of Wisconsin-Madison."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1301-8501","authenticated-orcid":true,"given":"Daniel D.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology (D.D.L., R.S.C., D.L.D., M.R.S., G.J.R.), Washington University School of Medicine, St. Louis, MO."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2475-3838","authenticated-orcid":true,"given":"Rafael S.","family":"Czepielewski","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology (D.D.L., R.S.C., D.L.D., M.R.S., G.J.R.), Washington University School of Medicine, St. Louis, MO."},{"name":"Now with Immunology Center of Georgia, Department of Physiology, Augusta University (R.S.C.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8206-0351","authenticated-orcid":true,"given":"Beth A.","family":"Helmink","sequence":"additional","affiliation":[{"name":"Department of Surgery (B.A.H., E.J.O.), Washington University School of Medicine, St. Louis, MO."},{"name":"Now with Department of Surgical Oncology, MD Anderson Cancer Center, Houston, TX (B.A.H.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8802-5346","authenticated-orcid":true,"given":"Emily J.","family":"Onufer","sequence":"additional","affiliation":[{"name":"Department of Surgery (B.A.H., E.J.O.), Washington University School of Medicine, St. Louis, MO."},{"name":"Now with Division of Pediatric General, Thoracic and Fetal Surgery, Children\u2019s Hospital of Philadelphia, PA (E.J.O.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deanna L.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology (D.D.L., R.S.C., D.L.D., M.R.S., G.J.R.), Washington University School of Medicine, St. Louis, MO."},{"name":"Now with Multidisciplinary and Professional Studies, Purdue Global, Indianapolis, IN (D.L.D.)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3899-5651","authenticated-orcid":true,"given":"Michael R.","family":"Strickland","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology (D.D.L., R.S.C., D.L.D., M.R.S., G.J.R.), Washington University School of Medicine, St. Louis, 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