{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:21:45Z","timestamp":1787768505916,"version":"build-2784847793"},"reference-count":57,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["ATVB"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND:<\/jats:title>\n                    <jats:p>\n                      MGP (matrix Gla protein) serves as an inhibitor of vascular calcification by limiting elastin degradation and regulating BMP (bone morphogenetic protein) activity. Mutation of the conserved proline residue 64 to glycine in MGP abolishes BMP binding in vitro. We hypothesized that selective loss of BMP binding would elucidate the contribution of BMP to cell differentiation in\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -deficient mice.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>\n                      Computational analyses using AlphaFold3 and molecular dynamics simulations were performed to determine the structural effects of \u03b3-carboxylation and the proline residue 64 to glycine mutation. The vascular phenotype of\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -knockin mice expressing the proline residue 64 to glycine mutation was compared with wild-type (WT) and global\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -knockout mice. Proximity ligation assay was performed to assess MGP-BMP4 in aortic cells. Single-cell RNA-sequencing was used to identify cellular alterations in the vascular media.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>\n                      Molecular dynamics simulation revealed 5 Ca\n                      <jats:sup>2+<\/jats:sup>\n                      ions coordinated by \u03b3-carboxylated Glu residues in the MGP dimer. The proline residue 64 to glycine mutation did not disrupt Ca\n                      <jats:sup>2+<\/jats:sup>\n                      binding but likely suppressed conformational changes required for BMP4 binding. Unlike\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -\n                      <jats:italic toggle=\"yes\">KO<\/jats:italic>\n                      mice,\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -knockin mice did not develop vascular calcification, elastic lamina proteolysis, and endothelial-mesenchymal transition, but exhibited vascular fibrosis. MGP-BMP4 interaction observed in WT aortic cells was barely detectable in\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -knockin aortic cells. Single-cell RNA-sequencing revealed increased fractions of smooth muscle cells and enhanced myofibroblast differentiation in the\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -knockin aortas compared with WT. In\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -knockin aortas, SMAD2 expression was significantly increased throughout the vessel wall compared with WT aortas. In contrast, SMAD1\/5\/9 activation in the\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -knockout aortas was more confined to the endothelium, whereas it localized to the media-adventitia transition in WT and\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -knockin aortas. Both\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -knockin and\n                      <jats:italic toggle=\"yes\">Mgp<\/jats:italic>\n                      -knockout mice developed arteriovenous malformations in the lungs, kidneys, and brain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS:<\/jats:title>\n                    <jats:p>Our findings suggest that MGP plays a versatile role in preserving vascular integrity, in part serving as an important BMP-trap aimed at directing vascular cell differentiation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/atvbaha.126.324621","type":"journal-article","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T09:00:16Z","timestamp":1784192416000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Tuning BMP-Regulated Cell Differentiation in the Aortic Media by Mutating Matrix Gla Protein"],"prefix":"10.1161","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8933-7133","authenticated-orcid":true,"given":"Xinjiang","family":"Cai","sequence":"first","affiliation":[{"name":"Division of Cardiology, Department of Medicine, David Geffen School of Medicine at the University of California, Los Angeles (X.C., L.Q., L.Z., X.W., Z.J., Y.Z., H.K., E.Y., T.K.H., Y.Y., K.I.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0242-0271","authenticated-orcid":true,"given":"Kavinda K.J.","family":"Gunasinghe","sequence":"additional","affiliation":[{"name":"Faculty of Engineering, Computing and Science, Swinburne University of Technology Sarawak, Kuching, Malaysia (K.K.J.G., X.W.C.)."},{"name":"Medicinal Chemistry, Monash Institute of Pharmaceutical Sciences, Monash University, Victoria, Australia (K.K.J.G.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5199-6236","authenticated-orcid":true,"given":"Lei","family":"Qi","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, David Geffen School of Medicine at the University of California, Los Angeles (X.C., L.Q., L.Z., X.W., Z.J., Y.Z., H.K., E.Y., T.K.H., Y.Y., K.I.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2638-2919","authenticated-orcid":true,"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, David Geffen School of Medicine at the University of California, Los Angeles (X.C., L.Q., L.Z., X.W., Z.J., Y.Z., H.K., E.Y., T.K.H., Y.Y., K.I.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7499-0137","authenticated-orcid":true,"given":"Xiuju","family":"Wu","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, David Geffen School of Medicine at the University of California, Los Angeles (X.C., L.Q., L.Z., X.W., Z.J., Y.Z., H.K., E.Y., T.K.H., Y.Y., K.I.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6501-0742","authenticated-orcid":true,"given":"Zheng","family":"Jing","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, David Geffen School of Medicine at the University of California, Los Angeles (X.C., L.Q., L.Z., X.W., Z.J., Y.Z., H.K., E.Y., T.K.H., Y.Y., K.I.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, David Geffen School of Medicine at the University of California, Los Angeles (X.C., L.Q., L.Z., X.W., Z.J., Y.Z., H.K., E.Y., T.K.H., Y.Y., K.I.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, David Geffen School of Medicine at the University of California, Los Angeles (X.C., L.Q., L.Z., X.W., Z.J., Y.Z., H.K., E.Y., T.K.H., Y.Y., K.I.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eddy","family":"Yao","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, David Geffen School of Medicine at the University of California, Los Angeles (X.C., L.Q., L.Z., X.W., Z.J., Y.Z., H.K., E.Y., T.K.H., Y.Y., K.I.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1734-0792","authenticated-orcid":true,"given":"Tzung K.","family":"Hsiai","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, David Geffen School of Medicine at the University of California, Los Angeles (X.C., L.Q., L.Z., X.W., Z.J., Y.Z., H.K., E.Y., T.K.H., Y.Y., K.I.B.)."},{"name":"Division of Cardiology, Department of Medicine, Greater Los Angeles VA Healthcare System, CA (T.K.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3830-5160","authenticated-orcid":true,"given":"Taufiq","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Cambridge, United Kingdom (T.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8497-5953","authenticated-orcid":true,"given":"Xavier W.","family":"Chee","sequence":"additional","affiliation":[{"name":"Faculty of Engineering, Computing and Science, Swinburne University of Technology Sarawak, Kuching, Malaysia (K.K.J.G., X.W.C.)."},{"name":"Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore (X.W.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0483-9180","authenticated-orcid":true,"given":"Yucheng","family":"Yao","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, David Geffen School of Medicine at the University of California, Los Angeles (X.C., L.Q., L.Z., X.W., Z.J., Y.Z., H.K., E.Y., T.K.H., Y.Y., K.I.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7745-5025","authenticated-orcid":true,"given":"Kristina I.","family":"Bostr\u00f6m","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, David Geffen School of Medicine at the University of California, Los Angeles (X.C., L.Q., L.Z., X.W., Z.J., Y.Z., H.K., E.Y., T.K.H., Y.Y., K.I.B.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.23.8335"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1989.69.3.990"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/386078a0"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.382"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.113.301792"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.306751"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.108.183053"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.495556"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2007.02758.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.107.166124"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.119.12412"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.122.317518"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00114714"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201612122"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI57567"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704297200"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1310905110"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.120.317439"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-021-04033-z"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008163"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.120.318049"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.01795"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a022210"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20945"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.3c01153"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/ct400341p"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.5b00743"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/stmcls\/sxac048"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13039-017-0328-2"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.176065"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11307-005-0015-2"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.121.317172"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-023-01767-y"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4402"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.44701"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02079"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-01-199166"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2020.01.043"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1802"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.114.304364"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2012.02.010"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI176379"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.124.070925"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2020.107637"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.4c09732"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2013.12.019"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2020.115542"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119830"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.09.014"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604239200"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2039"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0167936"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306972200"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.060930"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(00)02363-2"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2023.1210563"}],"container-title":["Arteriosclerosis, Thrombosis, and Vascular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/ATVBAHA.126.324621","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T18:00:56Z","timestamp":1787767256000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/ATVBAHA.126.324621"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9]]},"references-count":57,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1161\/ATVBAHA.126.324621"],"URL":"https:\/\/doi.org\/10.1161\/atvbaha.126.324621","relation":{},"ISSN":["1079-5642","1524-4636"],"issn-type":[{"value":"1079-5642","type":"print"},{"value":"1524-4636","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9]]},"assertion":[{"value":"2026-03-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}