{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T04:09:32Z","timestamp":1792642172529,"version":"4.5.0"},"reference-count":29,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","license":[{"start":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T00:00:00Z","timestamp":1696118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circ: Arrhythmia and Electrophysiology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:sec>\n            <jats:title>BACKGROUND:<\/jats:title>\n            <jats:p>\n              During electrophysiological mapping of tachycardias, putative target sites are often only truly confirmed to be vital after observing the effect of ablation. This lack of mapping specificity potentiates inadvertent ablation of innocent cardiac tissue not relevant to the arrhythmia. But if myocardial excitability could be transiently suppressed at critical regions, their suitability as targets could be conclusively determined before delivering tissue-destructive ablation lesions. We studied whether reversible pulsed electric fields (PF\n              <jats:sub>REV<\/jats:sub>\n              ) could transiently suppress electrical conduction, thereby providing a means to dissect tachycardia circuits in vivo.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>METHODS:<\/jats:title>\n            <jats:p>\n              PF\n              <jats:sub>REV<\/jats:sub>\n              energy was delivered from a 9-mm lattice-tip catheter to the atria of 12 swine and 9 patients, followed by serial electrogram assessments. The effects on electrical conduction were explored in 5 additional animals by applying PF\n              <jats:sub>REV<\/jats:sub>\n              to the atrioventricular node: 17 low-dose (PF\n              <jats:sub>REV-LOW<\/jats:sub>\n              ) and 10 high-dose (PF\n              <jats:sub>REV-HIGH<\/jats:sub>\n              ) applications. Finally, in 3 patients manifesting spontaneous tachycardias, PF\n              <jats:sub>REV<\/jats:sub>\n              was applied at putative critical sites.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>RESULTS:<\/jats:title>\n            <jats:p>\n              In animals, the immediate post-PF\n              <jats:sub>REV<\/jats:sub>\n              electrogram amplitudes diminished by 74%, followed by 78% recovery by 5 minutes. Similarly, in patients, a 69.9% amplitude reduction was followed by 84% recovery by 3 minutes. Histology revealed only minimal to no focal, superficial fibrosis. PF\n              <jats:sub>REV-LOW<\/jats:sub>\n              at the atrioventricular node resulted in transient PR prolongation and transient AV block in 59% and 6%, while PF\n              <jats:sub>REV-HIGH<\/jats:sub>\n              caused transient PR prolongation and transient AV block in 30% and 50%, respectively. The 3 tachycardia patients had atypical atrial flutters (n=2) and atrioventricular nodal reentrant tachycardia. PF\n              <jats:sub>REV<\/jats:sub>\n              at putative critical sites reproducibly terminated the tachycardias; ablation rendered the tachycardias noninducible and without recurrence during 1-year follow-up.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>CONCLUSIONS:<\/jats:title>\n            <jats:p>Reversible electroporation pulses can be applied to myocardial tissue to transiently block electrical conduction. This technique of pulsed field mapping may represent a novel electrophysiological tool to help identify the critical isthmus of tachycardia circuits.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circep.123.012018","type":"journal-article","created":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T09:01:06Z","timestamp":1695200466000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":37,"title":["Reversible Pulsed Electrical Fields as an In Vivo Tool to Study Cardiac Electrophysiology: The Advent of Pulsed Field Mapping"],"prefix":"10.1161","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2221-8372","authenticated-orcid":true,"given":"Jacob S.","family":"Koruth","sequence":"first","affiliation":[{"name":"Helmsley Electrophysiology Center, Icahn School of Medicine at Mount Sinai, New York, NY (J.S.K., I.K., K.K., V.Y.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4334-8165","authenticated-orcid":true,"given":"Petr","family":"Neuzil","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Homolka Hospital, Prague, Czech Republic (P.N., J.P., M.F., A.A., V.Y.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6431-9278","authenticated-orcid":true,"given":"Iwanari","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Helmsley Electrophysiology Center, Icahn School of Medicine at Mount Sinai, New York, NY (J.S.K., I.K., K.K., V.Y.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3257-8216","authenticated-orcid":true,"given":"Kenji","family":"Kuroki","sequence":"additional","affiliation":[{"name":"Helmsley Electrophysiology Center, Icahn School of Medicine at Mount Sinai, New York, NY (J.S.K., I.K., K.K., V.Y.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Petru","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Homolka Hospital, Prague, Czech Republic (P.N., J.P., M.F., A.A., V.Y.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3272-4380","authenticated-orcid":true,"given":"Gediminas","family":"Rackauskas","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Diseases, Vilnius University, Lithuania (G.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1620-3924","authenticated-orcid":true,"given":"Moritoshi","family":"Funasako","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Homolka Hospital, Prague, Czech Republic (P.N., J.P., M.F., A.A., V.Y.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrius","family":"Aidietis","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Homolka Hospital, Prague, Czech Republic (P.N., J.P., M.F., A.A., V.Y.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5638-4993","authenticated-orcid":true,"given":"Vivek Y.","family":"Reddy","sequence":"additional","affiliation":[{"name":"Helmsley Electrophysiology Center, Icahn School of Medicine at Mount Sinai, New York, NY (J.S.K., I.K., K.K., V.Y.R.)."},{"name":"Department of Cardiology, Homolka Hospital, Prague, Czech Republic (P.N., J.P., M.F., A.A., V.Y.R.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.107.748160"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioelechem.2021.107871"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hrthm.2015.05.012"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.116.004672"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.119.007781"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hrthm.2018.10.030"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.119.008303"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.120.008716"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/europace\/euab090"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacep.2018.04.005"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2019.04.021"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.119.008192"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2020.07.007"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.120.008718"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacep.2021.02.014"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCEP.121.010168"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/europace\/euac050"},{"key":"e_1_3_3_19_2","volume-title":"Application of Nanotechnology in Drug Delivery","author":"Bolhassani A","year":"2014","unstructured":"Bolhassani, A, Khavari, A, Orafa, Z. Electroporation \u2013 advantages and drawbacks for delivery of drug, gene and vaccine. In: Sezer, AD eds. Application of Nanotechnology in Drug Delivery. 2014, InTech."},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biophys-052118-115451"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(98)00987-9"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2004.05.012"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1997.273.6.H2817"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8167.1999.tb00247.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.66.5.1190"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)76974-2"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(99)76973-0"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00689.2003"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8159.1999.tb00353.x"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.102.23.2856"}],"container-title":["Circulation: Arrhythmia and Electrophysiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCEP.123.012018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T08:33:08Z","timestamp":1738830788000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCEP.123.012018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10]]},"references-count":29,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,10]]}},"alternative-id":["10.1161\/CIRCEP.123.012018"],"URL":"https:\/\/doi.org\/10.1161\/circep.123.012018","relation":{},"ISSN":["1941-3149","1941-3084"],"issn-type":[{"value":"1941-3149","type":"print"},{"value":"1941-3084","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10]]},"assertion":[{"value":"2023-01-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}