{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:20:36Z","timestamp":1792632036109,"version":"4.5.0"},"reference-count":61,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circ: Genomic and Precision Medicine"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:sec>\n            <jats:title>Background:<\/jats:title>\n            <jats:p>Hypertrophic cardiomyopathy (HCM) is characterized by asymmetric left ventricular hypertrophy. Currently, hypertrophy pathways responsible for HCM have not been fully elucidated. Their identification could serve as a nidus for the generation of novel therapeutics aimed at halting disease development or progression. Herein, we performed a comprehensive multi-omic characterization of hypertrophy pathways in HCM.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>Flash-frozen cardiac tissues were collected from genotyped HCM patients (n=97) undergoing surgical myectomy and tissue from 23 controls. RNA sequencing and mass spectrometry\u2013enabled deep proteome and phosphoproteomic assessment were performed. Rigorous differential expression, gene set enrichment, and pathway analyses were performed to characterize HCM-mediated alterations with emphasis on hypertrophy pathways.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>We identified transcriptional dysregulation with 1246 (8%) differentially expressed genes and elucidated downregulation of 10 hypertrophy pathways. Deep proteomic analysis identified 411 proteins (9%) that differed between HCM and controls with strong dysregulation of metabolic pathways. Seven hypertrophy pathways were upregulated with antagonistic upregulation of 5 of 10 hypertrophy pathways shown to be downregulated in the transcriptome. Most upregulated hypertrophy pathways encompassed the rat sarcoma-mitogen-activated protein kinase signaling cascade. Phosphoproteomic analysis demonstrated hyperphosphorylation of the rat sarcoma-mitogen-activated protein kinase system suggesting activation of this signaling cascade. There was a common transcriptomic and proteomic profile regardless of genotype.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>At time of surgical myectomy, the ventricular proteome, independent of genotype, reveals widespread upregulation and activation of hypertrophy pathways, mainly involving the rat sarcoma-mitogen-activated protein kinase signaling cascade. In addition, there is a counterregulatory transcriptional downregulation of the same pathways. Rat sarcoma-mitogen-activated protein kinase activation may serve a crucial role in hypertrophy observed in HCM.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circgen.122.003756","type":"journal-article","created":{"date-parts":[[2023,2,20]],"date-time":"2023-02-20T10:01:35Z","timestamp":1676887295000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":38,"title":["Multi-Omic Architecture of Obstructive Hypertrophic Cardiomyopathy"],"prefix":"10.1161","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5756-7417","authenticated-orcid":true,"given":"Ramin","family":"Garmany","sequence":"first","affiliation":[{"name":"Mayo Clinic Medical Scientist Training Program, Mayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic Alix School of Medicine, Rochester, MN (R.G.)."},{"name":"Department of Molecular Pharmacology and Experimental Therapeutics, Windland Smith Rice Sudden Death Genomics Laboratory, Rochester, MN (R.G., J.M.B., D.J.T., M.J.A.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3635-2003","authenticated-orcid":true,"given":"J. Martijn","family":"Bos","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Experimental Therapeutics, Windland Smith Rice Sudden Death Genomics Laboratory, Rochester, MN (R.G., J.M.B., D.J.T., M.J.A.)."},{"name":"Department of Cardiovascular Medicine (J.M.B., J.R.G., J.J.M., S.R.O., M.J.A.), Windland Smith Rice Genetic Heart Rhythm Clinic, Rochester, MN."},{"name":"Department of Pediatric and Adolescent Medicine, Division of Pediatric Cardiology (J.M.B., M.J.A.), Windland Smith Rice Genetic Heart Rhythm Clinic, Rochester, MN."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4554-2244","authenticated-orcid":true,"given":"David J.","family":"Tester","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology and Experimental Therapeutics, Windland Smith Rice Sudden Death Genomics Laboratory, Rochester, MN (R.G., J.M.B., D.J.T., M.J.A.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5871-0265","authenticated-orcid":true,"given":"John R.","family":"Giudicessi","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine (J.M.B., J.R.G., J.J.M., S.R.O., M.J.A.), Windland Smith Rice Genetic Heart Rhythm Clinic, Rochester, MN."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1052-7490","authenticated-orcid":true,"given":"Cristobal G.","family":"dos Remedios","sequence":"additional","affiliation":[{"name":"Mechanobiology Laboratory, Victor Chang Cardiac Research Institute, Darlinghurst, Australia (C.G.dR.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surendra","family":"Dasari","sequence":"additional","affiliation":[{"name":"Department of Quantitative Health Sciences, Division of Computational Biology (S.D., N.K.N., A.A.N.), Mayo Clinic, Rochester, MN."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4442-2585","authenticated-orcid":true,"given":"Nagaswaroop K.","family":"Nagaraj","sequence":"additional","affiliation":[{"name":"Department of Quantitative Health Sciences, Division of Computational Biology (S.D., N.K.N., A.A.N.), Mayo Clinic, Rochester, MN."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asha A.","family":"Nair","sequence":"additional","affiliation":[{"name":"Department of Quantitative Health Sciences, Division of Computational Biology (S.D., N.K.N., A.A.N.), Mayo Clinic, Rochester, MN."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8267-8047","authenticated-orcid":true,"given":"Kenneth L.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Proteomics Core (K.L.J., Z.C.R.), Mayo Clinic, Rochester, MN."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachary 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