{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T18:26:20Z","timestamp":1792002380398,"version":"4.4.0"},"reference-count":48,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circ Cardiovasc Genet"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:sec>\n            <jats:title>Background\u2014<\/jats:title>\n            <jats:p>After myocardial infarction, the left ventricle undergoes a wound healing response that includes the robust infiltration of neutrophils and macrophages to facilitate removal of dead myocytes as well as turnover of the extracellular matrix. Matrix metalloproteinase (MMP)-9 is a key enzyme that regulates post-myocardial infarction left ventricular remodeling.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods and Results\u2014<\/jats:title>\n            <jats:p>\n              Infarct regions from wild-type and MMP-9 null mice (n=8 per group) analyzed by glycoproteomics showed that of 541\n              <jats:italic>N<\/jats:italic>\n              -glycosylated proteins quantified, 45 proteins were at least 2-fold upregulated or downregulated with MMP-9 deletion (all\n              <jats:italic>P<\/jats:italic>\n              &lt;0.05). Cartilage intermediate layer protein and platelet glycoprotein 4 (CD36) were identified as having the highest fold increase in MMP-9 null mice. By immunoblotting, CD36 but not cartilage intermediate layer protein decreased steadily during the time course post-myocardial infarction, which identified CD36 as a candidate MMP-9 substrate. MMP-9 was confirmed in vitro and in vivo to proteolytically degrade CD36. In vitro stimulation of day 7 post-myocardial infarction macrophages with MMP-9 or a CD36-blocking peptide reduced phagocytic capacity. Dual immunofluorescence revealed concomitant accumulation of apoptotic neutrophils in the MMP-9 null group compared with wild-type group. In vitro stimulation of isolated neutrophils with MMP-9 decreased neutrophil apoptosis, indicated by reduced caspase-9 expression.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions\u2014<\/jats:title>\n            <jats:p>Our data reveal a new cell-signaling role for MMP-9 through CD36 degradation to regulate macrophage phagocytosis and neutrophil apoptosis.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circgenetics.115.001249","type":"journal-article","created":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T03:19:03Z","timestamp":1447816743000},"page":"14-25","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":94,"title":["CD36 Is a Matrix Metalloproteinase-9 Substrate That Stimulates Neutrophil Apoptosis and Removal During Cardiac Remodeling"],"prefix":"10.1161","volume":"9","author":[{"given":"Kristine Y.","family":"DeLeon-Pennell","sequence":"first","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Tian","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bai","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney A.","family":"Cates","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rugmani Padmanabhan","family":"Iyer","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Presley","family":"Cannon","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Punit","family":"Shah","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Aiyetan","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganesh V.","family":"Halade","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonggang","family":"Ma","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Flynn","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Fang","family":"Jin","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merry L.","family":"Lindsey","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Biophysics, Mississippi Center for Heart Research, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., M.L.L.); San Antonio Cardiovascular Proteomics Center, University of Mississippi Medical Center, Jackson (K.Y.D.-P., Y.T., C.A.C., R.P.I., P.C., Y.M., E.F., Y.-F.J., M.L.L.); Department of Electrical and Computer Engineering (Y.-F.J.), The University of Texas at San Antonio, San Antonio; Department of Pathology,..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0b013e31823ac046"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2010.07.010"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-505-7_18"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:HREV.0000011390.44039.b7"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0607338"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8768"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2003.09.042"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/13459"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900587"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.2174\/092986710793213724"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00457.2005"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81169-1"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.05.004"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt827"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.42"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0623181"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr2011268"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.3109\/10409239109081719"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2839"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp664"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh086"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn760"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/phy2.79"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2014.09.007"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2015.03.054"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01002.2010"},{"key":"e_1_3_4_30_2","first-page":"745","article-title":"Neutrophil isolation protocol.","volume":"17","author":"Oh H","year":"2008","unstructured":"Oh H, Siano B, Diamond S. Neutrophil isolation protocol. J Vis Exp. 2008;17:745.","journal-title":"J Vis Exp"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4547-10.2011"},{"key":"e_1_3_4_32_2","first-page":"3411","article-title":"The 72 kDa type IV collagenase is modulated via differential expression of alpha v beta 3 and alpha 5 beta 1 integrins during human melanoma cell invasion.","volume":"53","author":"Seftor RE","year":"1993","unstructured":"Seftor RE, Seftor EA, Stetler-Stevenson WG, Hendrix MJ. The 72 kDa type IV collagenase is modulated via differential expression of alpha v beta 3 and alpha 5 beta 1 integrins during human melanoma cell invasion. Cancer Res. 1993;53:3411\u20133415.","journal-title":"Cancer Res"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.148.1.78"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.11.6250"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/1755-1536-6-11"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2011.04.009"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI76246"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116019"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0904520"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2011.69"},{"key":"e_1_3_4_41_2","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1189\/jlb.68.6.873","article-title":"Spontaneous apoptosis in neutrophils is associated with downregulation of HLA Class I and is prevented by ligation of Class I.","volume":"68","author":"Frumento G","year":"2000","unstructured":"Frumento G, Ottonello L, Bertolotto M, Franchello S, Melioli G, Dallegri F. Spontaneous apoptosis in neutrophils is associated with downregulation of HLA Class I and is prevented by ligation of Class I. J Leukoc Biol. 2000;68:873\u2013880.","journal-title":"J Leukoc Biol"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113970"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)02324-3"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1132094100"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201200100"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0803212"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1112"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2006.02.055"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2014.10.008"}],"container-title":["Circulation: Cardiovascular Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCGENETICS.115.001249","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T01:28:25Z","timestamp":1715563705000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCGENETICS.115.001249"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1161\/CIRCGENETICS.115.001249"],"URL":"https:\/\/doi.org\/10.1161\/circgenetics.115.001249","relation":{},"ISSN":["1942-325X","1942-3268"],"issn-type":[{"value":"1942-325X","type":"print"},{"value":"1942-3268","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2]]}}}