{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:28:12Z","timestamp":1790072892138,"version":"4.0.1"},"reference-count":19,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"2","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circ: Cardiovascular Imaging"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Background\u2014<\/jats:italic>\n            <\/jats:bold>\n            Porcine bone marrow-derived mesenchymal stem cells (MSCs) were stably transfected with a lentiviral vector for transgene expression of the trifusion protein renilla luciferase, red fluorescent protein and herpes simplex truncated thymidine kinase (LV-RL-RFP-tTK; positron emission tomography [PET] reporter gene) for in vivo noninvasive tracking of the intramyocardially delivered MSC fate.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            A closed-chest, reperfused myocardial infarction was created in farm pigs. Sixteen days after myocardial infarction, LV-RL-RFP-tTK-MSCs were injected intramyocardially using electromechanical mapping guidance in the infarct border zone (n=7). PET-computed tomographic metabolic and perfusion imaging was performed after an intravenous injection of 10 mCi [18F]-FHBG and 13N-ammonia PET at 30\ufffd2 hours and 7 days after LV-RL-RFP-tTK-MSC treatment. Fusion imaging of the [18F]-FHBG PET-computed tomography with MRI was used to determine the myocardial location of the injected LV-RL-RFP-tTK-MSCs. Seven days after injections, [18F]-FHBG PET showed a decreased cardiac uptake with a mild increased pericardial and pleura uptake in the treated animals, which was confirmed by the measurement of luciferase activity. At 10 days, infarct size by MRI in the LV-RL-RFP-tTK-MSC-treated animals was smaller than controls (n=7) (23.3\ufffd1.5% versus 30.2\ufffd3.5%,\n            <jats:italic>P<\/jats:italic>\n            &lt;0.005). The presence of the LV-RL-RFP-tTK-MSCs (5.8\ufffd1.1% of the injected cells) in the myocardium 10 days after intramyocardial delivery was confirmed histologically.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            Reporter gene imaging enables the tracking of the persistence of viable LV-RL-RFP-tTK-MSC in the peri-infarcted porcine myocardium at 10 days after delivery using clinical PET scanners.\n          <\/jats:p>","DOI":"10.1161\/circimaging.108.797449","type":"journal-article","created":{"date-parts":[[2008,8,1]],"date-time":"2008-08-01T00:13:12Z","timestamp":1217549592000},"page":"94-103","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":136,"title":["Serial Noninvasive In Vivo Positron Emission Tomographic Tracking of Percutaneously Intramyocardially Injected Autologous Porcine Mesenchymal Stem Cells Modified for Transgene Reporter Gene Expression"],"prefix":"10.1161","volume":"1","author":[{"given":"Mariann","family":"Gyo\u0308ngyo\u0308si","sequence":"first","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeronimo","family":"Blanco","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tere\u0301z","family":"Marian","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lajos","family":"Tro\u0301n","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O\u0308rs","family":"Petneha\u0301zy","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsolt","family":"Petrasi","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rayyan","family":"Hemetsberger","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guszta\u0301v","family":"Font","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imre J.","family":"Pavo","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Istva\u0301n","family":"Kerte\u0301sz","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"La\u0301szlo\u0301","family":"Balkay","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noemi","family":"Pavo","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aniko","family":"Posa","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miklos","family":"Emri","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"La\u0301szlo\u0301","family":"Galuska","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dara L.","family":"Kraitchman","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johann","family":"Wojta","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt","family":"Huber","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietmar","family":"Glogar","sequence":"additional","affiliation":[{"name":"From the Department of Cardiology, Medical University of Vienna, Austria (M.G., R.H., I.J.P., N.P., A.P., J.W., D.G.); Centro de Investigacio\u0301n Cardiovascular (CSIC-ICCC), CIBER-BBN, Barcelona, Spain (J.B., J.R.); PET Centrum\u2014Institute of Nuclear Medicine of the University of Debrecen, Hungary (T.M., L.T., I.K., L.B., M.E., L.G.); Institute of Diagnostic Imaging and Radiation Oncology, University of Kaposvar, Hungary (O.P., Z.P.); Downtown Animal Clinic, Budapest, Hungary (G.F.); The Johns Hopkins..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16626-9"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa060186"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000084537.66419.7a"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-008-0704-5"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.21280"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000091252.20010.6e"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-1816"},{"key":"e_1_3_2_8_2","first-page":"415","volume":"4","year":"2003","unstructured":"Penuelas I, Boan JF, Marti-Climent JM, Barajas MA, Narvaiza I, Satyamurthy N, Barrio JR, Richter JA. 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