{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T07:49:11Z","timestamp":1792828151316,"version":"4.5.0"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circ: Cardiovascular Imaging"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:sec>\n            <jats:title>Background:<\/jats:title>\n            <jats:p>\n              We previously proposed a technique for quantitative measurement of rest and stress absolute myocardial blood flow (MBF) using a 2-injection single-scan imaging session. Recently, we validated the method in a pig model for the long-lived radiotracer\n              <jats:sup>18<\/jats:sup>\n              F-Flurpiridaz with adenosine as a pharmacological stressor. The aim of the present work is to validate our technique for\n              <jats:sup>13<\/jats:sup>\n              NH\n              <jats:sub>3<\/jats:sub>\n              .\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>\n              Nine studies were performed in 6 pigs; 5 studies were done in the native state and 4 after infarction of the left anterior descending artery. Each study consisted of 3 dynamic scans: a 2-injection rest-rest single-scan acquisition (scan A), a 2-injection rest\/stress single-scan acquisition (scan B), and a conventional 1-injection stress acquisition (scan C). Variable doses of adenosine combined with dobutamine were administered to induce a wide range of MBF. The 2-injection single-scan measurements were fitted with our nonstationary kinetic model (MGH2). In 4 studies,\n              <jats:sup>13<\/jats:sup>\n              NH\n              <jats:sub>3<\/jats:sub>\n              injections were paired with microsphere injections. MBF estimates obtained with our method were compared with those obtained with the standard method and with microspheres. We used a model-based method to generate separate rest and stress perfusion images.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>\n              In the absence of stress (scan A), the MBF values estimated by MGH2 were nearly the same for the 2-radiotracer injections (mean difference: 0.067\u00b10.070 mL\u00b7min\n              <jats:sup>\u22121<\/jats:sup>\n              \u00b7cc\n              <jats:sup>\u22121<\/jats:sup>\n              , limits of agreement: [\u22120.070 to 0.204] mL\u00b7min\n              <jats:sup>\u22121<\/jats:sup>\n              \u00b7cc\n              <jats:sup>\u22121<\/jats:sup>\n              ), showing good repeatability. Bland-Altman analyses demonstrated very good agreement with the conventional method for both rest (mean difference: \u22120.034\u00b10.035 mL\u00b7min\n              <jats:sup>\u22121<\/jats:sup>\n              \u00b7cc\n              <jats:sup>\u22121<\/jats:sup>\n              , limits of agreement: [\u22120.103 to 0.035] mL\u00b7min\n              <jats:sup>\u22121<\/jats:sup>\n              \u00b7cc\n              <jats:sup>\u22121<\/jats:sup>\n              ) and stress (mean difference: 0.057\u00b10.361 mL\u00b7min\n              <jats:sup>\u22121<\/jats:sup>\n              \u00b7cc\n              <jats:sup>\u22121<\/jats:sup>\n              , limits of agreement: [\u22120.651 to 0.765] mL\u00b7min\n              <jats:sup>\u22121<\/jats:sup>\n              \u00b7cc\n              <jats:sup>\u22121<\/jats:sup>\n              ) MBF measurements. Positron emission tomography and microsphere MBF measurements correlated closely. Very good quality perfusion images were obtained.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>\n              This study provides in vivo validation of our single-scan rest-stress method for\n              <jats:sup>13<\/jats:sup>\n              NH\n              <jats:sub>3<\/jats:sub>\n              measurements. The\n              <jats:sup>13<\/jats:sup>\n              NH\n              <jats:sub>3<\/jats:sub>\n              rest\/stress myocardial perfusion imaging procedure can be compressed into a single positron emission tomography scan session lasting less than 15 minutes.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circimaging.119.009407","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T10:00:32Z","timestamp":1579600832000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":7,"title":["Preclinical Validation of a Single-Scan Rest\/Stress Imaging Technique for\n            <sup>13<\/sup>\n            N-Ammonia Positron Emission Tomography Cardiac Perfusion Studies"],"prefix":"10.1161","volume":"13","author":[{"given":"Nicolas J.","family":"Guehl","sequence":"first","affiliation":[{"name":"Department of Radiology, Gordon Center for Medical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston (N.J.G., M.P.-G., D.W.W., J.L.G., M.D.N., G.E.F., N.M.A.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthieu","family":"Pelletier-Galarneau","sequence":"additional","affiliation":[{"name":"Department of Radiology, Gordon Center for Medical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston (N.J.G., M.P.-G., D.W.W., J.L.G., M.D.N., G.E.F., N.M.A.)."},{"name":"Department of Radiology and Nuclear Medicine, Montreal Heart Institute, Qu\u00e9bec, CA (M.P.-G.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dustin W.","family":"Wooten","sequence":"additional","affiliation":[{"name":"Department of Radiology, Gordon Center for Medical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston (N.J.G., M.P.-G., D.W.W., J.L.G., M.D.N., G.E.F., N.M.A.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Luis","family":"Guerrero","sequence":"additional","affiliation":[{"name":"Department of Radiology, Gordon Center for Medical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston (N.J.G., M.P.-G., D.W.W., J.L.G., M.D.N., G.E.F., N.M.A.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aur\u00e9lie","family":"Kas","sequence":"additional","affiliation":[{"name":"AP-HP, Department of Nuclear Medicine, Piti\u00e9-Salp\u00eatri\u00e8re Hospital, Sorbonne University, UPMC Paris 06, CNRS UMR 7371, INSERM U1146, France (A.K.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc D.","family":"Normandin","sequence":"additional","affiliation":[{"name":"Department of Radiology, Gordon Center for Medical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston (N.J.G., M.P.-G., D.W.W., J.L.G., M.D.N., G.E.F., N.M.A.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges El","family":"Fakhri","sequence":"additional","affiliation":[{"name":"Department of Radiology, Gordon Center for Medical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston (N.J.G., M.P.-G., D.W.W., J.L.G., M.D.N., G.E.F., N.M.A.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel M.","family":"Alpert","sequence":"additional","affiliation":[{"name":"Department of Radiology, Gordon Center for Medical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston (N.J.G., M.P.-G., D.W.W., J.L.G., M.D.N., G.E.F., N.M.A.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)61682-2"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.106.032789"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2005.01.040"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.02.068"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.01.065"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.050427"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1118\/1.4754585"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00259-017-3684-6"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.280.1.H108"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0033-0620(77)80005-4"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/hc0402.102975"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(90)90237-j"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(94)90629-7"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.80.5.1328"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90837-8"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/bf01068419"},{"key":"e_1_3_4_18_2","first-page":"212","article-title":"Assessment of the long-term reproducibility of baseline and dobutamine-induced myocardial blood flow in patients with stable coronary artery disease.","volume":"46","author":"Jagathesan R","year":"2005","unstructured":"Jagathesan, R, Kaufmann, PA, Rosen, SD, Rimoldi, OE, Turkeimer, F, Foale, R, Camici, PG. 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