{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T07:43:08Z","timestamp":1779176588874,"version":"3.51.4"},"reference-count":44,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circ: Cardiovascular Imaging"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:sec>\n            <jats:title>Background:<\/jats:title>\n            <jats:p>\n              The monocyte chemoattractant protein-1\/CCR2 (chemokine receptor 2) axis plays an important role in abdominal aortic aneurysm (AAA) pathogenesis, with effects on disease progression and anatomic stability. We assessed the expression of CCR2 in a rodent model and human tissues, using a targeted positron emission tomography radiotracer (\n              <jats:sup>64<\/jats:sup>\n              Cu-DOTA-ECL1i).\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>\n              AAAs were generated in Sprague-Dawley rats by exposing the infrarenal, intraluminal aorta to PPE (porcine pancreatic elastase) under pressure to induce aneurysmal degeneration. Heat-inactivated PPE was used to generate a sham operative control. Rat AAA rupture was stimulated by the administration of \u03b2-aminopropionitrile, a lysyl oxidase inhibitor. Biodistribution was performed in wild-type rats at 1 hour post tail vein injection of\n              <jats:sup>64<\/jats:sup>\n              Cu-DOTA-ECL1i. Dynamic positron emission tomography\/computed tomography imaging was performed in rats to determine the in vivo distribution of radiotracer.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>\n              Biodistribution showed fast renal clearance. The localization of radiotracer uptake in AAA was verified with high-resolution computed tomography. At day 7 post-AAA induction, the radiotracer uptake (standardized uptake value [SUV]=0.91\u00b10.25) was approximately twice that of sham-controls (SUV=0.47\u00b10.10;\n              <jats:italic>P<\/jats:italic>\n              &lt;0.01). At 14 days post-AAA induction, radiotracer uptake by either group did not significantly change (AAA SUV=0.86\u00b10.17 and sham-control SUV=0.46\u00b10.10), independent of variations in aortic diameter. Competitive CCR2 receptor blocking significantly decreased AAA uptake (SUV=0.42\u00b10.09). Tracer uptake in AAAs that subsequently ruptured (SUV=1.31\u00b10.14;\n              <jats:italic>P<\/jats:italic>\n              &lt;0.005) demonstrated uptake nearly twice that of nonruptured AAAs (SUV=0.73\u00b10.11). Histopathologic characterization of rat and human AAA tissues obtained from surgery revealed increased expression of CCR2 that was co-localized with CD68\n              <jats:sup>+<\/jats:sup>\n              macrophages. Ex vivo autoradiography demonstrated specific binding of\n              <jats:sup>64<\/jats:sup>\n              Cu-DOTA-ECL1i to CCR2 in both rat and human aortic tissues.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>CCR2 positron emission tomography is a promising new biomarker for the noninvasive assessment of AAA inflammation that may aid in associated rupture prediction.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circimaging.119.009889","type":"journal-article","created":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T09:00:48Z","timestamp":1584090048000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":44,"title":["CCR2 Positron Emission Tomography for the Assessment of Abdominal Aortic Aneurysm Inflammation and Rupture Prediction"],"prefix":"10.1161","volume":"13","author":[{"given":"Sean 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MO."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah","family":"Luehmann","sequence":"additional","affiliation":[{"name":"Department of Radiology (L.D., D.S., H.L., G.S.H., X.Z., R.L., J.Z., R.J.G., Y.L.), Washington University, St. Louis, MO."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Batool","family":"Arif","sequence":"additional","affiliation":[{"name":"Department of Surgery, Section of Vascular Surgery (S.J.E., S.E.S., B.A.), Washington University, St. Louis, MO."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gyu Seong","family":"Heo","sequence":"additional","affiliation":[{"name":"Department of Radiology (L.D., D.S., H.L., G.S.H., X.Z., R.L., J.Z., R.J.G., Y.L.), Washington University, St. Louis, MO."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Radiology (L.D., D.S., H.L., G.S.H., X.Z., R.L., J.Z., R.J.G., Y.L.), Washington University, St. Louis, MO."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Laforest","sequence":"additional","affiliation":[{"name":"Department of Radiology (L.D., D.S., H.L., G.S.H., X.Z., R.L., J.Z., R.J.G., Y.L.), Washington University, St. Louis, MO."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Radiology (L.D., D.S., H.L., G.S.H., X.Z., R.L., J.Z., R.J.G., Y.L.), Washington University, St. Louis, MO."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chieh-Yu","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Pathology and Immunology (C.-Y.L), Washington University, St. Louis, MO."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Gropler","sequence":"additional","affiliation":[{"name":"Department of Radiology (L.D., D.S., H.L., G.S.H., X.Z., R.L., J.Z., 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