{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T11:31:09Z","timestamp":1780054269670,"version":"3.54.0"},"reference-count":18,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circ: Cardiovascular Interventions"],"published-print":{"date-parts":[[2015,11]]},"abstract":"\n Background\u2014<\/jats:title>\n \n Allogenic platelet transfusions (PT) are administered to treat excessive bleeding in patients on P2Y\n 12<\/jats:sub>\n receptor inhibitors (RI). We assessed the effect of ex vivo and in vivo PT on platelet activation and aggregation in patients on dual antiplatelet therapy.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results\u2014<\/jats:title>\n \n In the Antagonize P2Y\n 12<\/jats:sub>\n Treatment Inhibitors by Transfusion of Platelets in an Urgent or Delayed Timing After Acute Coronary Syndrome or Percutaneous Coronary Intervention Presentation-Acute Coronary Syndrome (APTITUDE-ACS) study, patients presenting with acute coronary syndrome or for elective percutaneous coronary intervention, receiving loading doses of clopidogrel (600 mg, n=13 or 900 mg, n=12), prasugrel 60 mg (n=10), or ticagrelor 180 mg (n=10) were included. PT was performed ex vivo by mixing platelet-rich plasma from blood sampling performed at baseline in increasing proportions with platelet-rich plasma sampled 4 hours after loading dose. The percentage restoration of residual platelet aggregation achieved with 80% proportion PT (residual platelet aggregation 80% PT mix\/residual platelet aggregation baseline\u00d7100) significantly decreased with increasing potency of P2Y\n 12<\/jats:sub>\n RI (83.9\u00b111%, 73\u00b114%, 66.3\u00b115%, 40.9\u00b119% for clopidogrel 600 mg, clopidogrel 900 mg, prasugrel, and ticagrelor, respectively;\n P<\/jats:italic>\n for trend <0.0001). In the APTITUDE-Coronary Artery Bypass Graft (APTITUDE-CABG) study, vasodilator-stimulated phosphoprotein-platelet reactivity index, a specific marker of the P2Y\n 12<\/jats:sub>\n RI drug\u2013effect, was assessed before and after in vivo PT administered for excessive bleeding in patients undergoing cardiac surgery while on a maintenance dose of aspirin and clopidogrel (n=45), prasugrel (n=6), or ticagrelor (n=3). When compared with baseline, there was a significant relative increase of 23.1% in platelet activation after PT transfusion (42.2\u00b123.6% versus 56.6\u00b118.2%;\n P<\/jats:italic>\n =0.0008).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions\u2014<\/jats:title>\n \n PT restores platelet reactivity in patients with acute coronary syndrome\/percutaneous coronary intervention and in patients undergoing cardiac surgery on P2Y\n 12<\/jats:sub>\n RI while bleeding with a less effect with increasing potency of P2Y\n 12<\/jats:sub>\n inhibition.\n <\/jats:p>\n <\/jats:sec>\n \n Clinical Trial Registration\u2014<\/jats:title>\n \n URL:\n http:\/\/www.recherche-biomedicale.sante.gouv.fr\/pro\/comites\/coordonnees.htm<\/jats:ext-link>\n and\n http:\/\/www.cnil.fr\/<\/jats:ext-link>\n . Unique identifiers: No. 301111 and No. 1547216v0.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/circinterventions.115.002786","type":"journal-article","created":{"date-parts":[[2015,11,10]],"date-time":"2015-11-10T04:25:02Z","timestamp":1447129502000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":60,"title":["Efficacy of Ex Vivo Autologous and In Vivo Platelet Transfusion in the Reversal of P2Y\n 12<\/sub>\n Inhibition by Clopidogrel, Prasugrel, and Ticagrelor"],"prefix":"10.1161","volume":"8","author":[{"given":"Stephen A.","family":"O\u2019Connor","sequence":"first","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Amour","sequence":"additional","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Mercadier","sequence":"additional","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9jane","family":"Martin","sequence":"additional","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Kerneis","sequence":"additional","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00e9mie","family":"Abtan","sequence":"additional","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Brugier","sequence":"additional","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johanne","family":"Silvain","sequence":"additional","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Barth\u00e9l\u00e9my","sequence":"additional","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Leprince","sequence":"additional","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Montalescot","sequence":"additional","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Philippe","family":"Collet","sequence":"additional","affiliation":[{"name":"From the Sorbonne Universit\u00e9 UMPC (Paris 6), INSERM_UMRS1166 (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), ACTION Study Group, Paris, France (S.A.O., R.M., M.K., J.A., D.B., J.S., O.B., G.M., J.-P.C.), D\u00e9partement d\u2019Anesth\u00e9sie et de R\u00e9animation (J.A., R.M.), Etablissement Fran\u00e7ais Sang (A.M.), and Institut de Cardiologie and Chirurgie Cardiaque (S.A.O., M.K., J.A., D.B., J.S., O.B., P.L., G.M., J.-P.C.), Piti\u00e9-Salp\u00eatri\u00e8re Hospital (AP-HP), Paris, France."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67660-X"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000140675.85342.1B"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehu283"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2011.01.069"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.106.612812"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1308075"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.g6269"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehu158"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2006.06355.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/bja\/aet339"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2012.04641.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2011.04392.x"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.02245.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12058"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.776757"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.009449"},{"key":"e_1_3_3_18_2","first-page":"447","article-title":"Perioperative platelet transfusion. Recommendations of the French Health Products Safety Agency (AFSSAPS) 2003.","volume":"72","author":"Samama CM","year":"2006","unstructured":"Samama CM, Djoudi R, Lecompte T, Nathan N, Schved JF; French Health Products Safety Agency (AFSSAPS) Expert Group. Perioperative platelet transfusion. Recommendations of the French Health Products Safety Agency (AFSSAPS) 2003. Minerva Anestesiol. 2006;72:447\u2013452.","journal-title":"Minerva Anestesiol"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1409373"}],"container-title":["Circulation: Cardiovascular Interventions"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCINTERVENTIONS.115.002786","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T02:51:02Z","timestamp":1715568662000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCINTERVENTIONS.115.002786"}},"subtitle":["The APTITUDE Study"],"short-title":[],"issued":{"date-parts":[[2015,11]]},"references-count":18,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1161\/CIRCINTERVENTIONS.115.002786"],"URL":"https:\/\/doi.org\/10.1161\/circinterventions.115.002786","relation":{},"ISSN":["1941-7640","1941-7632"],"issn-type":[{"value":"1941-7640","type":"print"},{"value":"1941-7632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11]]},"assertion":[{"value":"2015-05-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-11-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e002786"}}