{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:58:21Z","timestamp":1791255501966,"version":"4.1.0"},"reference-count":44,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2007,11,26]]},"abstract":"<jats:p>\n            Nitric oxide has been shown to be an important signaling messenger in ischemic preconditioning (IPC). Accordingly, we investigated whether protein\n            <jats:italic>S<\/jats:italic>\n            -nitrosylation occurs in IPC hearts and whether\n            <jats:italic>S<\/jats:italic>\n            -nitrosoglutathione (GSNO) elicits similar effects on\n            <jats:italic>S<\/jats:italic>\n            -nitrosylation and cardioprotection. Preceding 20 minutes of no-flow ischemia and reperfusion, hearts from C57BL\/6J mice were perfused in the Langendorff mode and subjected to the following conditions: (1) control perfusion; (2) IPC; or (3) 0.1 mmol\/L GSNO treatment. Compared with control, IPC and GSNO significantly improved postischemic recovery of left ventricular developed pressure and reduced infarct size. IPC and GSNO both significantly increased\n            <jats:italic>S<\/jats:italic>\n            -nitrosothiol contents and\n            <jats:italic>S<\/jats:italic>\n            -nitrosylation levels of the L-type Ca\n            <jats:sup>2+<\/jats:sup>\n            channel \u03b11 subunit in heart membrane fractions. We identified several candidate\n            <jats:italic>S<\/jats:italic>\n            -nitrosylated proteins by proteomic analysis following the biotin switch method, including the cardiac sarcoplasmic reticulum Ca\n            <jats:sup>2+<\/jats:sup>\n            -ATPase, \u03b1-ketoglutarate dehydrogenase, and the mitochondrial F1-ATPase \u03b11 subunit. The activities of these enzymes were altered in a concentration-dependent manner by GSNO treatment. We further developed a 2D DyLight fluorescence difference gel electrophoresis proteomic method that used DyLight fluors and a modified biotin switch method to identify\n            <jats:italic>S<\/jats:italic>\n            -nitrosylated proteins. IPC and GSNO produced a similar pattern of\n            <jats:italic>S<\/jats:italic>\n            -nitrosylation modification and cardiac protection against ischemia\/reperfusion injury, suggesting that protein\n            <jats:italic>S<\/jats:italic>\n            -nitrosylation may play an important cardioprotective role in heart.\n          <\/jats:p>","DOI":"10.1161\/circresaha.107.155879","type":"journal-article","created":{"date-parts":[[2007,10,5]],"date-time":"2007-10-05T01:18:42Z","timestamp":1191547122000},"page":"1155-1163","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":350,"title":["Preconditioning Results in\n            <i>S<\/i>\n            -Nitrosylation of Proteins Involved in Regulation of Mitochondrial Energetics and Calcium Transport"],"prefix":"10.1161","volume":"101","author":[{"given":"Junhui","family":"Sun","sequence":"first","affiliation":[{"name":"From the Vascular Medicine Branch (J.S., E.M.) and Proteomics Core Facility (M.M., R.-F.S.), National Heart Lung and Blood Institute, NIH, Bethesda; and Department of Pathology (C.S.), Johns Hopkins University, Baltimore, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meghan","family":"Morgan","sequence":"additional","affiliation":[{"name":"From the Vascular Medicine Branch (J.S., E.M.) and Proteomics Core Facility (M.M., R.-F.S.), National Heart Lung and Blood Institute, NIH, Bethesda; and Department of Pathology (C.S.), Johns Hopkins University, Baltimore, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong-Fong","family":"Shen","sequence":"additional","affiliation":[{"name":"From the Vascular Medicine Branch (J.S., E.M.) and Proteomics Core Facility (M.M., R.-F.S.), National Heart Lung and Blood Institute, NIH, Bethesda; and Department of Pathology (C.S.), Johns Hopkins University, Baltimore, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Steenbergen","sequence":"additional","affiliation":[{"name":"From the Vascular Medicine Branch (J.S., E.M.) and Proteomics Core Facility (M.M., R.-F.S.), National Heart Lung and Blood Institute, NIH, Bethesda; and Department of Pathology (C.S.), Johns Hopkins University, Baltimore, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Murphy","sequence":"additional","affiliation":[{"name":"From the Vascular Medicine Branch (J.S., E.M.) and Proteomics Core Facility (M.M., R.-F.S.), National Heart Lung and Blood Institute, NIH, Bethesda; and Department of Pathology (C.S.), Johns Hopkins University, Baltimore, Md."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.74.5.1124"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.01.017"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2006.01.012"},{"key":"e_1_3_3_4_2","unstructured":"Liem DA Honda HM Zhang J Woo DD Ping P. 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