{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T23:48:06Z","timestamp":1792280886392,"version":"4.4.0"},"reference-count":44,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2008,9,12]]},"abstract":"<jats:p>\n            Homeobox transcription factor Nkx2-5, highly expressed in heart, is a critical factor during early embryonic cardiac development. In this study, using tamoxifen-inducible Nkx2-5 knockout mice, we demonstrate the role of Nkx2-5 in conduction and contraction in neonates within 4 days after perinatal tamoxifen injection. Conduction defect was accompanied by reduction in ventricular expression of the cardiac voltage-gated Na\n            <jats:sup>+<\/jats:sup>\n            channel pore-forming \u03b1-subunit (Na\n            <jats:sub>v<\/jats:sub>\n            1.5-\u03b1), the largest ion channel in the heart responsive for rapid depolarization of the action potential, which leads to increased intracellular Ca\n            <jats:sup>2+<\/jats:sup>\n            for contraction (conduction\u2013contraction coupling). In addition, expression of ryanodine receptor 2, through which Ca\n            <jats:sup>2+<\/jats:sup>\n            is released from sarcoplasmic reticulum, was substantially reduced in Nkx2-5 knockout mice. These results indicate that Nkx2-5 function is critical not only during cardiac development but also in perinatal hearts, by regulating expression of several important gene products involved in conduction and contraction.\n          <\/jats:p>","DOI":"10.1161\/circresaha.108.171835","type":"journal-article","created":{"date-parts":[[2008,8,9]],"date-time":"2008-08-09T00:54:25Z","timestamp":1218243265000},"page":"580-590","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":96,"title":["Perinatal Loss of Nkx2-5 Results in Rapid Conduction and Contraction Defects"],"prefix":"10.1161","volume":"103","author":[{"given":"Laura E.","family":"Briggs","sequence":"first","affiliation":[{"name":"From the Department of Physiology and Functional Genomics (L.E.B., M.T., A.E.C., M.H.M., A.J.W., T.S., S.P.O., C.S., M.K.R., H.K.), University of Florida College of Medicine, Gainesville; Department of Pediatrics and Developmental Biology (H.W.), Graduate School of Medicine, Tokyo Medical and Dental University, Japan; Cardiology Division (J.T.L.), University of California, San Francisco; Department of Radiation Oncology (N.H.), Washington University School of Medicine, St Louis, Mo; Cardiovascular..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morihiko","family":"Takeda","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Functional Genomics (L.E.B., M.T., A.E.C., M.H.M., A.J.W., T.S., S.P.O., C.S., M.K.R., H.K.), University of Florida College of Medicine, Gainesville; Department of Pediatrics and Developmental Biology (H.W.), Graduate School of Medicine, Tokyo Medical and Dental University, Japan; Cardiology Division (J.T.L.), University of California, San Francisco; Department of Radiation Oncology (N.H.), Washington University School of Medicine, St Louis, Mo; Cardiovascular..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adolfo E.","family":"Cuadra","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Functional Genomics (L.E.B., M.T., A.E.C., M.H.M., A.J.W., T.S., S.P.O., C.S., M.K.R., H.K.), University of Florida College of Medicine, Gainesville; Department of Pediatrics and Developmental Biology (H.W.), Graduate School of Medicine, Tokyo Medical and Dental University, Japan; Cardiology Division (J.T.L.), University of California, San Francisco; Department of Radiation Oncology (N.H.), Washington University School of Medicine, St Louis, Mo; Cardiovascular..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroko","family":"Wakimoto","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Functional Genomics (L.E.B., M.T., A.E.C., M.H.M., A.J.W., T.S., S.P.O., C.S., M.K.R., H.K.), University of Florida College of Medicine, Gainesville; Department of Pediatrics and Developmental Biology (H.W.), Graduate School of Medicine, Tokyo Medical and Dental University, Japan; Cardiology Division (J.T.L.), University of California, San Francisco; Department of Radiation Oncology (N.H.), Washington University School of Medicine, St Louis, Mo; Cardiovascular..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa H.","family":"Marks","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Functional Genomics (L.E.B., M.T., A.E.C., M.H.M., A.J.W., T.S., S.P.O., C.S., M.K.R., H.K.), University of Florida College of Medicine, Gainesville; Department of Pediatrics and Developmental Biology (H.W.), Graduate School of Medicine, Tokyo Medical and Dental University, Japan; Cardiology Division (J.T.L.), University of California, San Francisco; Department of Radiation Oncology (N.H.), Washington University School of Medicine, St Louis, Mo; Cardiovascular..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra J.","family":"Walker","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Functional Genomics (L.E.B., M.T., A.E.C., M.H.M., A.J.W., T.S., S.P.O., C.S., M.K.R., H.K.), University of Florida College of Medicine, Gainesville; Department of Pediatrics and Developmental Biology (H.W.), Graduate School of Medicine, Tokyo Medical and Dental University, Japan; Cardiology Division (J.T.L.), University of California, San Francisco; Department of Radiation Oncology (N.H.), Washington University School of Medicine, St Louis, Mo; Cardiovascular..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsugio","family":"Seki","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Functional Genomics (L.E.B., M.T., A.E.C., M.H.M., A.J.W., T.S., S.P.O., C.S., M.K.R., H.K.), University of Florida College of Medicine, Gainesville; Department of Pediatrics and Developmental Biology (H.W.), Graduate School of Medicine, Tokyo Medical and Dental University, Japan; Cardiology Division (J.T.L.), University of California, San Francisco; Department of Radiation Oncology (N.H.), Washington University School of Medicine, St Louis, Mo; Cardiovascular..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suk P.","family":"Oh","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Functional Genomics (L.E.B., M.T., A.E.C., M.H.M., A.J.W., T.S., S.P.O., C.S., M.K.R., H.K.), University of Florida College of Medicine, Gainesville; Department of Pediatrics and Developmental Biology (H.W.), Graduate School of Medicine, Tokyo Medical and Dental University, Japan; Cardiology Division (J.T.L.), University of California, San Francisco; Department of Radiation Oncology (N.H.), Washington University School of Medicine, St Louis, Mo; Cardiovascular..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan T.","family":"Lu","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Functional Genomics (L.E.B., M.T., A.E.C., M.H.M., A.J.W., T.S., S.P.O., C.S., M.K.R., H.K.), University of Florida College of Medicine, Gainesville; Department of Pediatrics and Developmental Biology (H.W.), Graduate School of Medicine, Tokyo Medical and Dental University, Japan; Cardiology Division (J.T.L.), University of California, San Francisco; Department of Radiation Oncology (N.H.), Washington University School of Medicine, St Louis, Mo; Cardiovascular..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Sumners","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Functional Genomics (L.E.B., M.T., A.E.C., M.H.M., A.J.W., T.S., S.P.O., C.S., M.K.R., H.K.), University of Florida College of Medicine, Gainesville; Department of Pediatrics and Developmental Biology (H.W.), Graduate School of Medicine, Tokyo Medical and Dental University, Japan; Cardiology Division (J.T.L.), University of California, San Francisco; Department of Radiation Oncology (N.H.), Washington University School of Medicine, St Louis, Mo; Cardiovascular..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohan K.","family":"Raizada","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Functional Genomics (L.E.B., M.T., A.E.C., M.H.M., A.J.W., T.S., S.P.O., C.S., M.K.R., H.K.), University of Florida College of Medicine, Gainesville; 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