{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:32:21Z","timestamp":1790861541634,"version":"4.1.0"},"reference-count":40,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2009,7,31]]},"abstract":"<jats:p>\n                    <jats:bold>\n                      <jats:italic>Rationale:<\/jats:italic>\n                    <\/jats:bold>\n                    Neurofibromatosis type 1 (NF1) is a common autosomal dominant disorder with a broad array of clinical manifestations, including benign and malignant tumors, and characteristic cutaneous findings. NF1 patients also have an increased incidence of cardiovascular diseases, including obstructive vascular disorders and hypertension. The disease gene,\n                    <jats:italic>NF1<\/jats:italic>\n                    , encodes neurofibromin, a ubiquitously expressed protein that acts, in part, as a Ras-GAP (GTP-ase activating protein), downregulating the activity of activated Ras protooncogenes. In animal models, endothelial and smooth muscle expression of the disease gene is critical for normal heart development and the prevention of vascular disease, respectively.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>\n                      <jats:italic>Objective:<\/jats:italic>\n                    <\/jats:bold>\n                    To determine the role of\n                    <jats:italic>NF1<\/jats:italic>\n                    in the postnatal and adult heart.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>\n                      <jats:italic>Methods and Results:<\/jats:italic>\n                    <\/jats:bold>\n                    We generated mice with homozygous loss of the murine homolog\n                    <jats:italic>Nf1<\/jats:italic>\n                    in myocardium (\n                    <jats:italic>Nf1<\/jats:italic>\n                    mKO) and evaluated their hearts for biochemical, structural, and functional changes.\n                    <jats:italic>Nf1<\/jats:italic>\n                    mKO mice have normal embryonic cardiovascular development but have marked cardiac hypertrophy, progressive cardiomyopathy, and fibrosis in the adult. Hyperactivation of Ras and downstream pathways are seen in the heart with the loss of\n                    <jats:italic>Nf1<\/jats:italic>\n                    , along with activation of a fetal gene program.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>\n                      <jats:italic>Conclusions:<\/jats:italic>\n                    <\/jats:bold>\n                    This report describes a critical role of\n                    <jats:italic>Nf1<\/jats:italic>\n                    in the regulation of cardiac growth and function. Activation of pathways known to be involved in cardiac hypertrophy and dysfunction are seen with the loss of myocardial neurofibromin.\n                  <\/jats:p>","DOI":"10.1161\/circresaha.109.201509","type":"journal-article","created":{"date-parts":[[2009,7,2]],"date-time":"2009-07-02T22:43:16Z","timestamp":1246574596000},"page":"304-311","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":49,"title":["Cardiomyocyte-Specific Loss of Neurofibromin Promotes Cardiac Hypertrophy and Dysfunction"],"prefix":"10.1161","volume":"105","author":[{"given":"Junwang","family":"Xu","sequence":"first","affiliation":[{"name":"From the Department of Cell and Developmental Biology and the Penn Cardiovascular Institute (J.X., F.A.I., T.W., M.M.L., N.A., J.A.E.), University of Pennsylvania, Philadelphia; and Division of Cardiology (F.A.I.), Department of Pediatrics, The Children\u2019s Hospital of Philadelphia, Pa. Present address for T.W.: GlaxoSmithKline, King of Prussia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fraz A.","family":"Ismat","sequence":"additional","affiliation":[{"name":"From the Department of Cell and Developmental Biology and the Penn Cardiovascular Institute (J.X., F.A.I., T.W., M.M.L., N.A., J.A.E.), University of Pennsylvania, Philadelphia; and Division of Cardiology (F.A.I.), Department of Pediatrics, The Children\u2019s Hospital of Philadelphia, Pa. Present address for T.W.: GlaxoSmithKline, King of Prussia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Wang","sequence":"additional","affiliation":[{"name":"From the Department of Cell and Developmental Biology and the Penn Cardiovascular Institute (J.X., F.A.I., T.W., M.M.L., N.A., J.A.E.), University of Pennsylvania, Philadelphia; and Division of Cardiology (F.A.I.), Department of Pediatrics, The Children\u2019s Hospital of Philadelphia, Pa. Present address for T.W.: GlaxoSmithKline, King of Prussia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Min","family":"Lu","sequence":"additional","affiliation":[{"name":"From the Department of Cell and Developmental Biology and the Penn Cardiovascular Institute (J.X., F.A.I., T.W., M.M.L., N.A., J.A.E.), University of Pennsylvania, Philadelphia; and Division of Cardiology (F.A.I.), Department of Pediatrics, The Children\u2019s Hospital of Philadelphia, Pa. Present address for T.W.: GlaxoSmithKline, King of Prussia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Antonucci","sequence":"additional","affiliation":[{"name":"From the Department of Cell and Developmental Biology and the Penn Cardiovascular Institute (J.X., F.A.I., T.W., M.M.L., N.A., J.A.E.), University of Pennsylvania, Philadelphia; and Division of Cardiology (F.A.I.), Department of Pediatrics, The Children\u2019s Hospital of Philadelphia, Pa. Present address for T.W.: GlaxoSmithKline, King of Prussia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan A.","family":"Epstein","sequence":"additional","affiliation":[{"name":"From the Department of Cell and Developmental Biology and the Penn Cardiovascular Institute (J.X., F.A.I., T.W., M.M.L., N.A., J.A.E.), University of Pennsylvania, Philadelphia; and Division of Cardiology (F.A.I.), Department of Pediatrics, The Children\u2019s Hospital of Philadelphia, Pa. 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