{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T22:55:04Z","timestamp":1781304904680,"version":"3.54.1"},"reference-count":57,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2010,8,6]]},"abstract":"<jats:sec>\n            <jats:title>\n              <jats:underline>Rationale<\/jats:underline>\n              :\n            <\/jats:title>\n            <jats:p>Binding of maternal anti-Ro\/La antibodies to cognate antigen expressed on apoptotic cardiocytes decreases clearance by healthy cardiocytes, which may contribute to the development of autoimmune associated congenital heart block and fatal cardiomyopathy.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Objective<\/jats:underline>\n              :\n            <\/jats:title>\n            <jats:p>Given recent evidence implicating the urokinase plasminogen activator receptor (uPAR) as a \u201cdon't eat me\u201d signal during efferocytosis, experiments addressed whether surface bound anti-Ro antibodies inhibit apoptotic cell removal via an effect on the expression\/function of the urokinase-type plasminogen activator protease uPA\/uPAR system.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Methods and Results<\/jats:underline>\n              :\n            <\/jats:title>\n            <jats:p>As assessed by flow cytometry and confocal microscopy, uPAR colocalizes and interacts with Ro60 on the surface of apoptotic human fetal cardiocytes. Blocking of uPAR enhances phagocytosis of apoptotic cardiocytes by healthy cardiocytes and reverses the anti-Ro60\u2013dependent impaired clearance of apoptotic cardiocytes. Binding of anti-Ro60 antibodies to apoptotic cardiocytes results in increased uPAR expression, as well as enhanced uPA activity. The binding of anti-Ro60 did not alter other surface molecules involved in cell recognition (calreticulin, CD31, or CD47).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Conclusions<\/jats:underline>\n              :\n            <\/jats:title>\n            <jats:p>These data suggest that increased uPAR expression and uPA activity induced by anti-Ro60 binding to the apoptotic fetal cardiocyte provide a molecular basis by which these antibodies inhibit efferocytosis and ultimately lead to scar of the fetal conduction system and working myocardium.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circresaha.109.213629","type":"journal-article","created":{"date-parts":[[2010,6,18]],"date-time":"2010-06-18T02:53:26Z","timestamp":1276829606000},"page":"374-387","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":28,"title":["Role of the Urokinase Plasminogen Activator Receptor in Mediating Impaired Efferocytosis of Anti-SSA\/Ro\u2013Bound Apoptotic Cardiocytes"],"prefix":"10.1161","volume":"107","author":[{"given":"Paraskevi","family":"Briassouli","sequence":"first","affiliation":[{"name":"From the Department of Medicine, Division of Rheumatology, New York University School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena V.","family":"Komissarova","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Division of Rheumatology, New York University School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Clancy","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Division of Rheumatology, New York University School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill P.","family":"Buyon","sequence":"additional","affiliation":[{"name":"From the Department of Medicine, Division of Rheumatology, New York University School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_5_2_2","first-page":"139","article-title":"Cardiac manifestations of neonatal lupus erythematosus: guidelines to management, integrating clues from the bench and bedside","volume":"5","author":"Buyon JP","year":"2009","unstructured":"Buyon JP, Clancy RM, Friedman DM . 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