{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T11:04:51Z","timestamp":1783335891987,"version":"3.54.6"},"reference-count":53,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2010,11,12]]},"abstract":"<jats:sec>\n            <jats:title>\n              <jats:underline>Rationale and Objective:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>The M-band represents a transverse structure in the center of the sarcomeric A-band and provides an anchor for the myosin-containing thick filaments. In contrast to other sarcomeric structures, eg, the Z-disc, only few M-band\u2013specific proteins have been identified to date, and its exact molecular composition remains unclear.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Methods and Results:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              Using a bioinformatic approach to identify novel heart- and muscle-specific genes, we found a leucine rich protein, myomasp (\n              <jats:underline>Myo<\/jats:underline>\n              sin-interacting,\n              <jats:underline>M<\/jats:underline>\n              -band-\n              <jats:underline>a<\/jats:underline>\n              ssociated\n              <jats:underline>s<\/jats:underline>\n              tress-responsive\n              <jats:underline>p<\/jats:underline>\n              rotein)\/LRRC39. RT-PCR and Northern and Western blot analyses confirmed a cardiac-enriched expression pattern, and immunolocalization of myomasp revealed a strong and specific signal at the sarcomeric M-band. Yeast 2-hybrid screens, as well as coimmunoprecipitation experiments, identified the C terminus of myosin heavy chain (MYH)7 as an interaction partner for myomasp. Knockdown of myomasp in neonatal rat ventricular myocytes (NRVCMs) led to a significant upregulation of the stretch-sensitive genes GDF-15 and BNP. Conversely, the expression of MYH7 and the M-band proteins myomesin-1 and -2 was found to be markedly reduced. Mechanistically, knockdown of myomasp in NRVCM led to a dose-dependent suppression of serum response factor\u2013dependent gene expression, consistent with earlier observations linking the M-band to serum response factor\u2013mediated signaling. Finally, downregulation of myomasp\/LRRC39 in spontaneously beating engineered heart tissue constructs resulted in significantly lower force generation and reduced fractional shortening. Likewise, knockdown of the myomasp\/LRRC39 ortholog in zebrafish resulted in severely impaired heart function and cardiomyopathy in vivo.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Conclusions:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>These findings reveal myomasp as a previously unrecognized component of an M-band\u2013associated signaling pathway that regulates cardiomyocyte gene expression in response to biomechanical stress.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circresaha.110.222372","type":"journal-article","created":{"date-parts":[[2010,9,17]],"date-time":"2010-09-17T04:32:09Z","timestamp":1284697929000},"page":"1253-1264","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":47,"title":["Myomasp\/LRRC39, a Heart- and Muscle-Specific Protein, Is a Novel Component of the Sarcomeric M-Band and Is Involved in Stretch Sensing"],"prefix":"10.1161","volume":"107","author":[{"given":"Rainer D.","family":"Will","sequence":"first","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Eden","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Just","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arne","family":"Hansen","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Eder","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derk","family":"Frank","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Kuhn","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thalia S.","family":"Seeger","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Oehl","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; 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Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Koegl","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Rottbauer","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Eschenhagen","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; Department of Cardiology (M.E., C.K., N.F.), University Hospital Schleswig-Holstein, Campus Kiel, Germany; Department of Experimental and Clinical Pharmacology (A.H., A.E., T.E.), University Medical Center Hamburg-Eppendorf, Hamburg; Department of Internal Medicine II (W.R.), University Hospital Ulm; and Department of Molecular Genome Analysis (S.W.) and Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo A.","family":"Katus","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine III (R.D.W., M.E., S.J., D.F., T.S.S., U.O., W.R., H.A.K.), University of Heidelberg; 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