{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:31:05Z","timestamp":1790364665231,"version":"4.1.0"},"reference-count":37,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2010,9,3]]},"abstract":"<jats:sec>\n            <jats:title>\n              <jats:underline>Rationale:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>Stromal cell\u2013derived factor (SDF)-1\/CXCR4 axis has an instrumental role during cardiac development and has been shown to be a potential therapeutic target for optimizing ventricular remodeling after acute myocardial infarction (AMI) and in ischemic cardiomyopathy. Although a therapeutic target, the specific role of cardiac myocyte CXCR4 (CM-CXCR4) expression following cardiogenesis and survival of cardiac myocyte and left ventricular remodeling after AMI is unknown.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Objective:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              We hypothesized that cardiac myocyte derived CXCR4 is critical for cardiac development, but it may have no role in adulthood secondary to the short transient expression of SDF-1 and the delayed expression of CM-CXCR4 following AMI. To address this issue, we developed congenital and conditional CM-CXCR4\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mouse models.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Methods and Results:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              Two strains of CM-CXCR4\n              <jats:sup>flox\/flox<\/jats:sup>\n              mice were generated by crossing CXCR4\n              <jats:sup>flox\/flox<\/jats:sup>\n              mice with MCM-Cre\n              <jats:sup>+\/\u2212<\/jats:sup>\n              mouse and MLC2v-Cre\n              <jats:sup>+\/\u2212<\/jats:sup>\n              mouse on the C57BL\/6J background, yielding CXCR4\n              <jats:sup>flox\/flox<\/jats:sup>\n              MCM-Cre\n              <jats:sup>+\/\u2212<\/jats:sup>\n              and CXCR4\n              <jats:sup>flox\/flox<\/jats:sup>\n              MLC2v-Cre\n              <jats:sup>+\/\u2212<\/jats:sup>\n              mice. Studies demonstrated recombination in both models congenitally in the MLC2v-Cre\n              <jats:sup>+\/\u2212<\/jats:sup>\n              mice and following tamoxifen administration in the MCM-Cre\n              <jats:sup>+\/\u2212<\/jats:sup>\n              mice. Surprisingly the CXCR4\n              <jats:sup>flox\/flox<\/jats:sup>\n              MLC2v-Cre\n              <jats:sup>+\/\u2212<\/jats:sup>\n              are viable, had normal cardiac function, and had no evidence of ventricular septal defect. CXCR4\n              <jats:sup>flox\/flox<\/jats:sup>\n              MCM\n              <jats:sup>+\/\u2212<\/jats:sup>\n              treated with tamoxifen 2 weeks before AMI demonstrated 90% decrease in cardiac CXCR4 expression 48 hours after AMI. Twenty-one days post AMI, echocardiography revealed no statistically significant difference in the wall thickness, left ventricular dimensions or ejection fraction (40.9\u00b17.5 versus 34.4\u00b12.6%) in CXCR4\n              <jats:sup>flox\/flox<\/jats:sup>\n              mice versus CM-CXCR4\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice regardless of strategy of Cre expression. No differences in vascular density (2369\u00b1131 versus 2471\u00b1126 vessels\/mm\n              <jats:sup>2<\/jats:sup>\n              ; CXCR4\n              <jats:sup>flox\/flox<\/jats:sup>\n              versus CM-CXCR4\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mouse), infarct size, collagen content, or noninfarct zone cardiac myocyte size were observed 21 days after AMI.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Conclusions:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>We conclude that cardiac myocyte\u2013derived CXCR4 is not essential for cardiac development and, potentially because of the mismatch in timings of peaks of SDF-1 and CXCR4, has no major role in ventricular remodeling after AMI.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circresaha.110.223289","type":"journal-article","created":{"date-parts":[[2010,7,16]],"date-time":"2010-07-16T03:17:55Z","timestamp":1279250275000},"page":"667-676","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":67,"title":["Role of Cardiac Myocyte CXCR4 Expression in Development and Left Ventricular Remodeling After Acute Myocardial Infarction"],"prefix":"10.1161","volume":"107","author":[{"given":"Udit","family":"Agarwal","sequence":"first","affiliation":[{"name":"From the Skirball Laboratory for Cardiovascular Cellular Therapeutics, Departments of Cardiovascular Medicine and Stem Cell Biology and Regenerative Medicine (U.A., W.G., F.D., K.W., M.S.P.); and Center for Cardiovascular Cell Therapy, Heart and Vascular Institute (M.S.P.), Cleveland Clinic, Ohio; Department of Genetics Medicine (S.Y.R., S.R.) and Howard Hughes Medical Institute (S.R.), Weill Medical College of Cornell University, New York; Feinstein Laboratory for Hematopoiesis (Y.R.Z.), Long..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wael","family":"Ghalayini","sequence":"additional","affiliation":[{"name":"From the Skirball Laboratory for Cardiovascular Cellular Therapeutics, Departments of Cardiovascular Medicine and Stem Cell Biology and Regenerative Medicine (U.A., W.G., F.D., K.W., M.S.P.); and Center for Cardiovascular Cell Therapy, Heart and Vascular Institute (M.S.P.), Cleveland Clinic, Ohio; Department of Genetics Medicine (S.Y.R., S.R.) and Howard Hughes Medical Institute (S.R.), Weill Medical College of Cornell University, New York; Feinstein Laboratory for Hematopoiesis (Y.R.Z.), Long..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Dong","sequence":"additional","affiliation":[{"name":"From the Skirball Laboratory for Cardiovascular Cellular Therapeutics, Departments of Cardiovascular Medicine and Stem Cell Biology and Regenerative Medicine (U.A., W.G., F.D., K.W., M.S.P.); and Center for Cardiovascular Cell Therapy, Heart and Vascular Institute (M.S.P.), Cleveland Clinic, Ohio; Department of Genetics Medicine (S.Y.R., S.R.) and Howard Hughes Medical Institute (S.R.), Weill Medical College of Cornell University, New York; Feinstein Laboratory for Hematopoiesis (Y.R.Z.), Long..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristal","family":"Weber","sequence":"additional","affiliation":[{"name":"From the Skirball Laboratory for Cardiovascular Cellular Therapeutics, Departments of Cardiovascular Medicine and Stem Cell Biology and Regenerative Medicine (U.A., W.G., F.D., K.W., M.S.P.); and Center for Cardiovascular Cell Therapy, Heart and Vascular Institute (M.S.P.), Cleveland Clinic, Ohio; Department of Genetics Medicine (S.Y.R., S.R.) and Howard Hughes Medical Institute (S.R.), Weill Medical College of Cornell University, New York; Feinstein Laboratory for Hematopoiesis (Y.R.Z.), Long..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Rui","family":"Zou","sequence":"additional","affiliation":[{"name":"From the Skirball Laboratory for Cardiovascular Cellular Therapeutics, Departments of Cardiovascular Medicine and Stem Cell Biology and Regenerative Medicine (U.A., W.G., F.D., K.W., M.S.P.); and Center for Cardiovascular Cell Therapy, Heart and Vascular Institute (M.S.P.), Cleveland Clinic, Ohio; Department of Genetics Medicine (S.Y.R., S.R.) and Howard Hughes Medical Institute (S.R.), Weill Medical College of Cornell University, New York; Feinstein Laboratory for Hematopoiesis (Y.R.Z.), Long..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sina Y.","family":"Rabbany","sequence":"additional","affiliation":[{"name":"From the Skirball Laboratory for Cardiovascular Cellular Therapeutics, Departments of Cardiovascular Medicine and Stem Cell Biology and Regenerative Medicine (U.A., W.G., F.D., K.W., M.S.P.); and Center for Cardiovascular Cell Therapy, Heart and Vascular Institute (M.S.P.), Cleveland Clinic, Ohio; Department of Genetics Medicine (S.Y.R., S.R.) and Howard Hughes Medical Institute (S.R.), Weill Medical College of Cornell University, New York; Feinstein Laboratory for Hematopoiesis (Y.R.Z.), Long..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahin","family":"Rafii","sequence":"additional","affiliation":[{"name":"From the Skirball Laboratory for Cardiovascular Cellular Therapeutics, Departments of Cardiovascular Medicine and Stem Cell Biology and Regenerative Medicine (U.A., W.G., F.D., K.W., M.S.P.); and Center for Cardiovascular Cell Therapy, Heart and Vascular Institute (M.S.P.), Cleveland Clinic, Ohio; Department of Genetics Medicine (S.Y.R., S.R.) and Howard Hughes Medical Institute (S.R.), Weill Medical College of Cornell University, New York; Feinstein Laboratory for Hematopoiesis (Y.R.Z.), Long..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc S.","family":"Penn","sequence":"additional","affiliation":[{"name":"From the Skirball Laboratory for Cardiovascular Cellular Therapeutics, Departments of Cardiovascular Medicine and Stem Cell Biology and Regenerative Medicine (U.A., W.G., F.D., K.W., M.S.P.); and Center for Cardiovascular Cell Therapy, Heart and Vascular Institute (M.S.P.), Cleveland Clinic, Ohio; Department of Genetics Medicine (S.Y.R., S.R.) and Howard Hughes Medical Institute (S.R.), Weill Medical College of Cornell University, New York; Feinstein Laboratory for Hematopoiesis (Y.R.Z.), Long..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000043246.74879.cd"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000095788.78169.af"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa060186"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-109462"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14232-8"},{"key":"e_1_3_4_7_2","first-page":"729","article-title":"Expression of SDF-1-CXCR4 axis and an anti-remodelling effectiveness of foetal-liver stem cell transplantation in the infarcted rat heart","volume":"58","author":"Czarnowska E","year":"2007","unstructured":"Czarnowska E, Gajerska-Dzieciatkowska M, Kusmierski K, Lichomski J, Machaj EK, Pojda Z, Brudek M, Beresewicz A. 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