{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T05:31:13Z","timestamp":1792042273877,"version":"4.4.0"},"reference-count":45,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2010,10,15]]},"abstract":"<jats:sec>\n            <jats:title>\n              <jats:underline>Rationale:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>A critical event in the development of cardiac fibrosis is the transformation of fibroblasts into myofibroblasts. The electrophysiological consequences of this phenotypic switch remain largely unknown.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Objective:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>Determine whether fibroblast activation following cardiac injury results in a distinct electrophysiological phenotype that enhances fibroblast\u2013myocyte interactions.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Methods and Results:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              Neonatal rat myocyte monolayers were treated with media (CM) conditioned by fibroblasts isolated from normal (Fb) and infarcted (MI-Fb) hearts. Fb and MI-Fb were also plated on top of myocyte monolayers at 3 densities. Cultures were optically mapped after CM treatment or fibroblast plating to obtain conduction velocity and action potential duration (APD\n              <jats:sub>70<\/jats:sub>\n              ). Intercellular communication and connexin43 expression levels were assessed. Membrane properties of Fb and MI-Fb were evaluated using patch clamp techniques. MI-Fb CM treatment decreased conduction velocity (11.1%) compared to untreated myocyte cultures. APD\n              <jats:sub>70<\/jats:sub>\n              was reduced by MI-Fb CM treatment compared to homocellular myocyte culture (9.4%) and Fb CM treatment (6.4%). In heterocellular cultures, MI-Fb conduction velocities were different from Fb at all densities (+29.8%, \u221223.0%, and \u221216.7% at 200, 400, and 600 cells\/mm\n              <jats:sup>2<\/jats:sup>\n              , respectively). APD\n              <jats:sub>70<\/jats:sub>\n              was reduced (9.6%) in MI-Fb compared to Fb cultures at 200 cells\/mm\n              <jats:sup>2<\/jats:sup>\n              . MI-Fb had more hyperpolarized resting membrane potentials and increased outward current densities. Connexin43 was elevated (134%) in MI-Fb compared to Fb. Intercellular coupling evaluated with gap fluorescence recovery after photobleaching was higher between myocytes and MI-Fb compared to Fb.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Conclusions:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>These data demonstrate cardiac injury results in significant electrophysiological changes that enhance fibroblast\u2013myocyte interactions and could contribute to the greater incidence of arrhythmias observed in fibrotic hearts.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circresaha.110.227421","type":"journal-article","created":{"date-parts":[[2010,8,13]],"date-time":"2010-08-13T02:50:59Z","timestamp":1281667859000},"page":"1011-1020","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":164,"title":["Enhanced Fibroblast\u2013Myocyte Interactions in Response to Cardiac Injury"],"prefix":"10.1161","volume":"107","author":[{"given":"Carolina","family":"Vasquez","sequence":"first","affiliation":[{"name":"From the Leon H. Charney Division of Cardiology, New York University School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Poornima","family":"Mohandas","sequence":"additional","affiliation":[{"name":"From the Leon H. Charney Division of Cardiology, New York University School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen L.","family":"Louie","sequence":"additional","affiliation":[{"name":"From the Leon H. Charney Division of Cardiology, New York University School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najate","family":"Benamer","sequence":"additional","affiliation":[{"name":"From the Leon H. Charney Division of Cardiology, New York University School of Medicine."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashwini C.","family":"Bapat","sequence":"additional","affiliation":[{"name":"From the Leon H. 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