{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T04:40:12Z","timestamp":1792730412644,"version":"4.5.0"},"reference-count":30,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2012,1,6]]},"abstract":"<jats:sec>\n            <jats:title>\n              <jats:underline>Rationale:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>Endothelial apoptosis is increased in association with acute and chronic vascular rejection (VR) of solid allografts. Apoptotic endothelial cells (EC) release LG3, a C-terminal fragment of perlecan of potential importance in vascular remodeling and neointima formation.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Objective:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>Our 2 goals were to determine whether circulating levels of LG3 are increased in association with acute VR of renal allografts and to evaluate the impact of LG3 on vascular remodeling.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Methods and Results:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              We conducted a case-control study to compare serum LG3 levels in human renal transplant patients with acute VR, tubulo-interstitial rejection (ATIR) and normal graft function. Aorta transplantation between fully MHC-mismatched mice in association with intravenous LG3 injection was used to characterize the impact of LG3 on vascular remodeling. Scratch assays evaluated the promigratory activity of LG3 on vascular smooth muscle cells (VSMC) in vitro. Serum LG3 levels were significantly elevated in human renal transplant patients with acute VR (\n              <jats:italic>n<\/jats:italic>\n              =16) compared to ATIR (\n              <jats:italic>n<\/jats:italic>\n              =16) and normal graft function (\n              <jats:italic>n<\/jats:italic>\n              =32,\n              <jats:italic>P<\/jats:italic>\n              =0.004). In patients with acute VR, graft loss was associated with elevated LG3 levels. Increasing LG3 serum levels in aortic allograft recipients significantly increased neointima formation. LG3 injection fostered accumulation of \u03b1-smooth muscle actin\u2013positive cells and decreased the number of CD31 positive EC. LG3 increased the migration of VSMC through extracellular signal-regulated kinases 1\/2-dependent pathways.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Conclusion:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>These results indicate that LG3 is a novel regulator of obliterative vascular remodeling during rejection.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circresaha.111.250431","type":"journal-article","created":{"date-parts":[[2011,11,11]],"date-time":"2011-11-11T02:59:02Z","timestamp":1320980342000},"page":"94-104","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":74,"title":["The Perlecan Fragment LG3 Is a Novel Regulator of Obliterative Remodeling Associated With Allograft Vascular Rejection"],"prefix":"10.1161","volume":"110","author":[{"given":"Mathilde","family":"Soulez","sequence":"first","affiliation":[{"name":"From the Research Centre, Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)\u2014H\u00f4pital Notre Dame and Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (M.S., E.-A.P., M.D., H.C., N.B., S.Q., S.-J.W., M.-J.H.); Biotechnology Research Institute, Montr\u00e9al, Quebec, Canada (Y.D.); Research Centre, H\u00f4pital du Sacr\u00e9 C\u0153ur de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (F.M.); and Institut de Recherche en Immunologie et Canc\u00e9rologie (IRIC), Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (C.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eve-Annie","family":"Pilon","sequence":"additional","affiliation":[{"name":"From the Research Centre, Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)\u2014H\u00f4pital Notre Dame and Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (M.S., E.-A.P., M.D., H.C., N.B., S.Q., S.-J.W., M.-J.H.); Biotechnology Research Institute, Montr\u00e9al, Quebec, Canada (Y.D.); Research Centre, H\u00f4pital du Sacr\u00e9 C\u0153ur de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (F.M.); and Institut de Recherche en Immunologie et Canc\u00e9rologie (IRIC), Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (C.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lanie","family":"Dieud\u00e9","sequence":"additional","affiliation":[{"name":"From the Research Centre, Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)\u2014H\u00f4pital Notre Dame and Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (M.S., E.-A.P., M.D., H.C., N.B., S.Q., S.-J.W., M.-J.H.); Biotechnology Research Institute, Montr\u00e9al, Quebec, Canada (Y.D.); Research Centre, H\u00f4pital du Sacr\u00e9 C\u0153ur de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (F.M.); and Institut de Recherche en Immunologie et Canc\u00e9rologie (IRIC), Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (C.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9lo\u00efse","family":"Cardinal","sequence":"additional","affiliation":[{"name":"From the Research Centre, Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)\u2014H\u00f4pital Notre Dame and Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (M.S., E.-A.P., M.D., H.C., N.B., S.Q., S.-J.W., M.-J.H.); Biotechnology Research Institute, Montr\u00e9al, Quebec, Canada (Y.D.); Research Centre, H\u00f4pital du Sacr\u00e9 C\u0153ur de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (F.M.); and Institut de Recherche en Immunologie et Canc\u00e9rologie (IRIC), Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (C.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Brassard","sequence":"additional","affiliation":[{"name":"From the Research Centre, Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)\u2014H\u00f4pital Notre Dame and Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (M.S., E.-A.P., M.D., H.C., N.B., S.Q., S.-J.W., M.-J.H.); Biotechnology Research Institute, Montr\u00e9al, Quebec, Canada (Y.D.); Research Centre, H\u00f4pital du Sacr\u00e9 C\u0153ur de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (F.M.); and Institut de Recherche en Immunologie et Canc\u00e9rologie (IRIC), Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (C.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shijie","family":"Qi","sequence":"additional","affiliation":[{"name":"From the Research Centre, Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)\u2014H\u00f4pital Notre Dame and Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (M.S., E.-A.P., M.D., H.C., N.B., S.Q., S.-J.W., M.-J.H.); Biotechnology Research Institute, Montr\u00e9al, Quebec, Canada (Y.D.); Research Centre, H\u00f4pital du Sacr\u00e9 C\u0153ur de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (F.M.); and Institut de Recherche en Immunologie et Canc\u00e9rologie (IRIC), Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (C.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyh-Jong","family":"Wu","sequence":"additional","affiliation":[{"name":"From the Research Centre, Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)\u2014H\u00f4pital Notre Dame and Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (M.S., E.-A.P., M.D., H.C., N.B., S.Q., S.-J.W., M.-J.H.); Biotechnology Research Institute, Montr\u00e9al, Quebec, Canada (Y.D.); Research Centre, H\u00f4pital du Sacr\u00e9 C\u0153ur de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (F.M.); and Institut de Recherche en Immunologie et Canc\u00e9rologie (IRIC), Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (C.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Durocher","sequence":"additional","affiliation":[{"name":"From the Research Centre, Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)\u2014H\u00f4pital Notre Dame and Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (M.S., E.-A.P., M.D., H.C., N.B., S.Q., S.-J.W., M.-J.H.); Biotechnology Research Institute, Montr\u00e9al, Quebec, Canada (Y.D.); Research Centre, H\u00f4pital du Sacr\u00e9 C\u0153ur de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (F.M.); and Institut de Recherche en Immunologie et Canc\u00e9rologie (IRIC), Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (C.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Madore","sequence":"additional","affiliation":[{"name":"From the Research Centre, Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)\u2014H\u00f4pital Notre Dame and Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (M.S., E.-A.P., M.D., H.C., N.B., S.Q., S.-J.W., M.-J.H.); Biotechnology Research Institute, Montr\u00e9al, Quebec, Canada (Y.D.); Research Centre, H\u00f4pital du Sacr\u00e9 C\u0153ur de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (F.M.); and Institut de Recherche en Immunologie et Canc\u00e9rologie (IRIC), Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (C.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Perreault","sequence":"additional","affiliation":[{"name":"From the Research Centre, Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)\u2014H\u00f4pital Notre Dame and Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (M.S., E.-A.P., M.D., H.C., N.B., S.Q., S.-J.W., M.-J.H.); Biotechnology Research Institute, Montr\u00e9al, Quebec, Canada (Y.D.); Research Centre, H\u00f4pital du Sacr\u00e9 C\u0153ur de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (F.M.); and Institut de Recherche en Immunologie et Canc\u00e9rologie (IRIC), Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (C.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Jos\u00e9e","family":"H\u00e9bert","sequence":"additional","affiliation":[{"name":"From the Research Centre, Centre Hospitalier de l'Universit\u00e9 de Montr\u00e9al (CRCHUM)\u2014H\u00f4pital Notre Dame and Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (M.S., E.-A.P., M.D., H.C., N.B., S.Q., S.-J.W., M.-J.H.); Biotechnology Research Institute, Montr\u00e9al, Quebec, Canada (Y.D.); Research Centre, H\u00f4pital du Sacr\u00e9 C\u0153ur de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (F.M.); and Institut de Recherche en Immunologie et Canc\u00e9rologie (IRIC), Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Quebec, Canada (C.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2005.01165.x"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000246086.93555.f3"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pathmechdis.3.121806.151508"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(200002)190:3<300::AID-PATH596>3.0.CO;2-I"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1053\/ajkd.2002.29865"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.108.171488"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181bca1e4"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2009.09.002"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(01)00151-2"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djj441"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801164200"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M409841200"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M606412200"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.03-0573fje"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.403"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2004.00710.x"},{"key":"e_1_3_5_18_2","first-page":"921","article-title":"Human transplant coronary artery disease: pathological evidence for fas-mediated apoptotic cytotoxicity in allograft arteriopathy","volume":"74","author":"Dong C","year":"1996","unstructured":"Dong C, Wilson JE, Winters GL, McManus BM . Human transplant coronary artery disease: pathological evidence for fas-mediated apoptotic cytotoxicity in allograft arteriopathy. 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