{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T14:12:14Z","timestamp":1791209534244,"version":"4.1.0"},"reference-count":57,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2012,3,30]]},"abstract":"<jats:sec>\n            <jats:title>\n              <jats:underline>Rationale:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              Naturally occurring mutations in the cardiac ryanodine receptor (RyR2) have been associated with both cardiac arrhythmias and cardiomyopathies. It is clear that delayed afterdepolarization resulting from abnormal activation of sarcoplasmic reticulum Ca\n              <jats:sup>2+<\/jats:sup>\n              release is the primary cause of RyR2-associated cardiac arrhythmias. However, the mechanism underlying RyR2-associated cardiomyopathies is completely unknown.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Objective:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              In the present study, we investigate the role of the NH\n              <jats:sub>2<\/jats:sub>\n              -terminal region of RyR2 in and the impact of a number of cardiomyopathy-associated RyR2 mutations on the termination of Ca\n              <jats:sup>2+<\/jats:sup>\n              release.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Methods and Results:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              The 35-residue exon-3 region of RyR2 is associated with dilated cardiomyopathy. Single-cell luminal Ca\n              <jats:sup>2+<\/jats:sup>\n              imaging revealed that the deletion of the first 305 NH\n              <jats:sub>2<\/jats:sub>\n              -terminal residues encompassing exon-3 or the deletion of exon-3 itself markedly reduced the luminal Ca\n              <jats:sup>2+<\/jats:sup>\n              threshold at which Ca\n              <jats:sup>2+<\/jats:sup>\n              release terminates and increased the fractional Ca\n              <jats:sup>2+<\/jats:sup>\n              release. Single-cell cytosolic Ca\n              <jats:sup>2+<\/jats:sup>\n              imaging also showed that both RyR2 deletions enhanced the amplitude of store overload-induced Ca\n              <jats:sup>2+<\/jats:sup>\n              transients in HEK293 cells or HL-1 cardiac cells. Furthermore, the RyR2 NH\n              <jats:sub>2<\/jats:sub>\n              -terminal mutations, A77V, R176Q\/T2504M, R420W, and L433P, which are associated with arrhythmogenic right ventricular displasia type 2, also reduced the threshold for Ca\n              <jats:sup>2+<\/jats:sup>\n              release termination and increased fractional release. The RyR2 A1107M mutation associated with hypertrophic cardiomyopathy had the opposite action (ie, increased the threshold for Ca\n              <jats:sup>2+<\/jats:sup>\n              release termination and reduced fractional release).\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Conclusions:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              These results provide the first evidence that the NH\n              <jats:sub>2<\/jats:sub>\n              -terminal region of RyR2 is an important determinant of Ca\n              <jats:sup>2+<\/jats:sup>\n              release termination, and that abnormal fractional Ca\n              <jats:sup>2+<\/jats:sup>\n              release attributable to aberrant termination of Ca\n              <jats:sup>2+<\/jats:sup>\n              release is a common defect in RyR2-associated cardiomyopathies.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circresaha.111.256560","type":"journal-article","created":{"date-parts":[[2012,2,29]],"date-time":"2012-02-29T05:53:08Z","timestamp":1330494788000},"page":"968-977","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":114,"title":["Abnormal Termination of Ca\n            <sup>2+<\/sup>\n            Release Is a Common Defect of RyR2 Mutations Associated With Cardiomyopathies"],"prefix":"10.1161","volume":"110","author":[{"given":"Yijun","family":"Tang","sequence":"first","affiliation":[{"name":"From the Department of Physiology and Pharmacology (Y.T., X.T., R.W., S.R.W.C.), the Libin Cardiovascular Institute of Alberta, University of Calgary, Calgary, AB, Canada; Department of Molecular Biophysics and Physiology (M.F., S.R.W.C.), Rush University Medical Center, Chicago, IL. Y.T. is currently affiliated with Taihe Hospital Affiliated Hospital of Hubei University of Medicine, Hubei, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xixi","family":"Tian","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Pharmacology (Y.T., X.T., R.W., S.R.W.C.), the Libin Cardiovascular Institute of Alberta, University of Calgary, Calgary, AB, Canada; Department of Molecular Biophysics and Physiology (M.F., S.R.W.C.), Rush University Medical Center, Chicago, IL. Y.T. is currently affiliated with Taihe Hospital Affiliated Hospital of Hubei University of Medicine, Hubei, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiwu","family":"Wang","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Pharmacology (Y.T., X.T., R.W., S.R.W.C.), the Libin Cardiovascular Institute of Alberta, University of Calgary, Calgary, AB, Canada; Department of Molecular Biophysics and Physiology (M.F., S.R.W.C.), Rush University Medical Center, Chicago, IL. Y.T. is currently affiliated with Taihe Hospital Affiliated Hospital of Hubei University of Medicine, Hubei, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Fill","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Pharmacology (Y.T., X.T., R.W., S.R.W.C.), the Libin Cardiovascular Institute of Alberta, University of Calgary, Calgary, AB, Canada; Department of Molecular Biophysics and Physiology (M.F., S.R.W.C.), Rush University Medical Center, Chicago, IL. Y.T. is currently affiliated with Taihe Hospital Affiliated Hospital of Hubei University of Medicine, Hubei, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.R. Wayne","family":"Chen","sequence":"additional","affiliation":[{"name":"From the Department of Physiology and Pharmacology (Y.T., X.T., R.W., S.R.W.C.), the Libin Cardiovascular Institute of Alberta, University of Calgary, Calgary, AB, Canada; Department of Molecular Biophysics and Physiology (M.F., S.R.W.C.), Rush University Medical Center, Chicago, IL. Y.T. is currently affiliated with Taihe Hospital Affiliated Hospital of Hubei University of Medicine, Hubei, PR China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/415198a"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2003.12.002"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvm009"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2006.08.115"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8167.2007.00766.x"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.226845"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2009.08.022"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.107.711606"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-10-12"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.3.189"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(02)01946-0"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.4065\/79.11.1380"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2005.04.019"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1253\/circj.70.1402"},{"key":"e_1_3_4_16_2","first-page":"165","article-title":"A novel missense mutation in cardiac ryanodine receptor gene as a possible cause of hypertrophic cardiomyopathy: Evidence from familial analysis","volume":"114","author":"Noboru F","year":"2006","unstructured":"Noboru F, Hidekazu I, Kenshi H, Katsuharu U, Mitsuru N, Tetsuo K, Hiromasa K, Yuichiro S, Toshinari T, Kazuo O, Sumio M, Masakazu Y . A novel missense mutation in cardiac ryanodine receptor gene as a possible cause of hypertrophic cardiomyopathy: Evidence from familial analysis. Circulation. 2006; 114: 165.","journal-title":"Circulation"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(82)84557-8"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.82.1.119"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/304735a0"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.23.7367"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/26.3.193"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.003bo.x"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402388101"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000192146.85173.4b"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000079967.11815.19"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500059102"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.107.183236"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceca.2004.01.013"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2009.177576"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvm038"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2011.06.024"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.017129"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.125pe31"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11093"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.07.164"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.107.156380"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(89)90358-2"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408030101"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20071287"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.108.180612"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.4161\/chan.4.4.12666"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.103.2.196"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0600268103"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.108.177493"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvq007"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.109.209312"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.25.15096"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000032490.04207.bd"},{"key":"e_1_3_4_49_2","first-page":"3893","article-title":"Myofilament Ca2+ sensitization causes susceptibility to cardiac arrhythmia in mice","volume":"118","author":"Baudenbacher F","year":"2008","unstructured":"Baudenbacher F, Schober T, Pinto JR, Sidorov VY, Hilliard F, Solaro RJ, Potter JD, Knollmann BC . Myofilament Ca2+ sensitization causes susceptibility to cardiac arrhythmia in mice. J Clin Invest. 2008; 118: 3893\u20133903.","journal-title":"J Clin Invest"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2010.01.011"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.109.935296"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.87.9.774"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0905186106"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2009.08.016"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature09471"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2011.03.016"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000085"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504298102"}],"container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCRESAHA.111.256560","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T03:35:50Z","timestamp":1715571350000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCRESAHA.111.256560"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,3,30]]},"references-count":57,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2012,3,30]]}},"alternative-id":["10.1161\/CIRCRESAHA.111.256560"],"URL":"https:\/\/doi.org\/10.1161\/circresaha.111.256560","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,3,30]]},"assertion":[{"value":"2011-09-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-02-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-02-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}