{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:31:51Z","timestamp":1791286311568,"version":"4.1.0"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2014,3,14]]},"abstract":"<jats:sec>\n                    <jats:title>\n                      <jats:underline>Rationale:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>Our previous study has shown that yes-associated protein (YAP) plays a crucial role in the phenotypic modulation of vascular smooth muscle cells (SMCs) in response to arterial injury. However, the role of YAP in vascular SMC development is unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>\n                      <jats:underline>Objective:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>The goal of this study was to investigate the functional role of YAP in cardiovascular development in mice and determine the mechanisms underlying YAP\u2019s actions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>\n                      <jats:underline>Methods and Results:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>YAP was deleted in cardiomyocytes and vascular SMCs by crossing YAP flox mice with SM22\u03b1-Cre transgenic mice. Cardiac\/SMC-specific deletion of YAP directed by SM22\u03b1-Cre resulted in perinatal lethality in mice because of profound cardiac defects including hypoplastic myocardium, membranous ventricular septal defect, and double outlet right ventricle. The cardiac\/SMC-specific YAP knockout mice also displayed severe vascular abnormalities including hypoplastic arterial wall, short\/absent brachiocephalic artery, and retroesophageal right subclavian artery. Deletion of YAP in mouse vascular SMCs induced expression of a subset of cell cycle arrest genes including G-protein\u2013coupled receptor 132 (Gpr132). Silencing Gpr132 promoted SMC proliferation, whereas overexpression of Gpr132 attenuated SMC growth by arresting cell cycle in G0\/G1 phase, suggesting that ablation of YAP-induced impairment of SMC proliferation was mediated, at least in part, by induction of Gpr132 expression. Mechanistically, YAP recruited the epigenetic repressor histone deacetylase-4 to suppress Gpr132 gene expression via a muscle CAT element in the Gpr132 gene.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>\n                      <jats:underline>Conclusions:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>YAP plays a critical role in cardiac\/SMC proliferation during cardiovascular development by epigenetically regulating expression of a set of cell cycle suppressors.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/circresaha.114.303411","type":"journal-article","created":{"date-parts":[[2014,1,30]],"date-time":"2014-01-30T00:22:02Z","timestamp":1391041322000},"page":"957-965","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":122,"title":["Deletion of Yes-Associated Protein (YAP) Specifically in Cardiac and Vascular Smooth Muscle Cells Reveals a Crucial Role for YAP in Mouse Cardiovascular Development"],"prefix":"10.1161","volume":"114","author":[{"given":"Yong","family":"Wang","sequence":"first","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqing","family":"Hu","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Liu","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobo","family":"Wang","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingfu","family":"Wu","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Schwarz","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiliang","family":"Zhou","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Medical College of Georgia, Georgia Regents University, Augusta (Y.W., G.H., F.L., J.Z.); and Center for Cardiovascular Sciences, Albany Medical College, NY (X.W., M.W., J.J.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.250878"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/000025622"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.110.223800"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1909210"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2010.09.011"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.07.019"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2008.10.001"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1199010"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1116136109"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2002278"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.436311"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1313192110"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.112.254730"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)67861-0"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.71.4.759"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2010.06.015"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.102650499"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.26.1.77-87.2006"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.78.2.188"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.110.221952"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.113.01843"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20120152"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00041.2003"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2007.11.018"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/gene.20112"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2005.06.004"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(01)00198-X"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.112.300200"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.21.12334"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M209101200"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1061781"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200711145"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00449.2006"}],"container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCRESAHA.114.303411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T23:20:41Z","timestamp":1715556041000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCRESAHA.114.303411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,14]]},"references-count":33,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,3,14]]}},"alternative-id":["10.1161\/CIRCRESAHA.114.303411"],"URL":"https:\/\/doi.org\/10.1161\/circresaha.114.303411","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,14]]},"assertion":[{"value":"2014-01-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}