{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:23:31Z","timestamp":1791408211130,"version":"4.3.3"},"reference-count":50,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2014,10,10]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale:<\/jats:title>\n                    <jats:p>Cardiac progenitor cells from the second heart field (SHF) contribute to rapid growth of the embryonic heart, giving rise to right ventricular and outflow tract (OFT) myocardium at the arterial pole of the heart, and atrial myocardium at the venous pole. Recent clonal analysis and cell-tracing experiments indicate that a common progenitor pool in the posterior region of the SHF gives rise to both OFT and atrial myocytes. The mechanisms regulating deployment of this progenitor pool remain unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      To evaluate the role of\n                      <jats:italic>TBX1<\/jats:italic>\n                      , the major gene implicated in congenital heart defects in 22q11.2 deletion syndrome patients, in posterior SHF development.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results:<\/jats:title>\n                    <jats:p>\n                      Using transcriptome analysis, genetic tracing, and fluorescent dye-labeling experiments, we show that\n                      <jats:italic>Tbx1<\/jats:italic>\n                      -dependent OFT myocardium originates in\n                      <jats:italic>Hox<\/jats:italic>\n                      -expressing cells in the posterior SHF. In\n                      <jats:italic>Tbx1<\/jats:italic>\n                      null embryos, OFT progenitor cells fail to segregate from this progenitor cell pool, leading to failure to expand the dorsal pericardial wall and altered positioning of the cardiac poles. Unexpectedly, addition of SHF cells to the venous pole of the heart is also impaired, resulting in abnormal development of the dorsal mesenchymal protrusion, and partially penetrant atrioventricular septal defects, including ostium primum defects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Tbx1<\/jats:italic>\n                      is required for inflow as well as OFT morphogenesis by regulating the segregation and deployment of progenitor cells in the posterior SHF. Our results provide new insights into the pathogenesis of congenital heart defects and 22q11.2 deletion syndrome phenotypes.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/circresaha.115.305020","type":"journal-article","created":{"date-parts":[[2014,9,5]],"date-time":"2014-09-05T01:20:52Z","timestamp":1409880052000},"page":"790-799","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":151,"title":["Tbx1 Coordinates Addition of Posterior Second Heart Field Progenitor Cells to the Arterial and Venous Poles of the Heart"],"prefix":"10.1161","volume":"115","author":[{"given":"M. Sameer","family":"Rana","sequence":"first","affiliation":[{"name":"From the Department of Anatomy, Embryology and Physiology, Heart Failure Research Center, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands (M.S.R., V.M.C.); CNRS, IBDM UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K.) and Inserm, GMGF UMR S910, Facult\u00e9 de M\u00e9decine de la Timone (M.R., B.L., S.Z.), Aix Marseille Universit\u00e9, IBDM, CNRS UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K) and GMGF Inserm URM S910 (M.R., B.L., S.Z.), Marseille, France; Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Th\u00e9veniau-Ruissy","sequence":"additional","affiliation":[{"name":"From the Department of Anatomy, Embryology and Physiology, Heart Failure Research Center, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands (M.S.R., V.M.C.); CNRS, IBDM UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K.) and Inserm, GMGF UMR S910, Facult\u00e9 de M\u00e9decine de la Timone (M.R., B.L., S.Z.), Aix Marseille Universit\u00e9, IBDM, CNRS UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K) and GMGF Inserm URM S910 (M.R., B.L., S.Z.), Marseille, France; Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"De Bono","sequence":"additional","affiliation":[{"name":"From the Department of Anatomy, Embryology and Physiology, Heart Failure Research Center, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands (M.S.R., V.M.C.); CNRS, IBDM UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K.) and Inserm, GMGF UMR S910, Facult\u00e9 de M\u00e9decine de la Timone (M.R., B.L., S.Z.), Aix Marseille Universit\u00e9, IBDM, CNRS UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K) and GMGF Inserm URM S910 (M.R., B.L., S.Z.), Marseille, France; Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karim","family":"Mesbah","sequence":"additional","affiliation":[{"name":"From the Department of Anatomy, Embryology and Physiology, Heart Failure Research Center, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands (M.S.R., V.M.C.); CNRS, IBDM UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K.) and Inserm, GMGF UMR S910, Facult\u00e9 de M\u00e9decine de la Timone (M.R., B.L., S.Z.), Aix Marseille Universit\u00e9, IBDM, CNRS UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K) and GMGF Inserm URM S910 (M.R., B.L., S.Z.), Marseille, France; 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CNRS, IBDM UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K.) and Inserm, GMGF UMR S910, Facult\u00e9 de M\u00e9decine de la Timone (M.R., B.L., S.Z.), Aix Marseille Universit\u00e9, IBDM, CNRS UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K) and GMGF Inserm URM S910 (M.R., B.L., S.Z.), Marseille, France; Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Mohun","sequence":"additional","affiliation":[{"name":"From the Department of Anatomy, Embryology and Physiology, Heart Failure Research Center, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands (M.S.R., V.M.C.); CNRS, IBDM UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K.) and Inserm, GMGF UMR S910, Facult\u00e9 de M\u00e9decine de la Timone (M.R., B.L., S.Z.), Aix Marseille Universit\u00e9, IBDM, CNRS UMR 7288 (M.T.-R., C.D.B., K.M., A.F., M.R., R.G.K) and GMGF Inserm URM S910 (M.R., B.L., S.Z.), Marseille, France; Department of..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephane","family":"Zaffran","sequence":"additional","affiliation":[{"name":"From the Department of Anatomy, Embryology and Physiology, Heart Failure Research Center, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands (M.S.R., V.M.C.); 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