{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T18:02:05Z","timestamp":1792692125134,"version":"4.5.0"},"reference-count":54,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:sec>\n            <jats:title>\n              <jats:underline>Rationale:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              Inflamed atherosclerotic plaques can be visualized by noninvasive positron emission and computed tomographic imaging with\n              <jats:sup>18<\/jats:sup>\n              F-fluorodeoxyglucose, a glucose analog, but the underlying mechanisms are poorly understood.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Objective:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              Here, we directly investigated the role of Glut1-mediated glucose uptake in apolipoprotein E\u2013deficient (\n              <jats:italic>ApoE<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              ) mouse model of atherosclerosis.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Methods and Results:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              We first showed that the enhanced glycolytic flux in atheromatous plaques of\n              <jats:italic>ApoE<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice was associated with the enhanced metabolic activity of hematopoietic stem and multipotential progenitor cells and higher Glut1 expression in these cells. Mechanistically, the regulation of Glut1 in\n              <jats:italic>ApoE<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              hematopoietic stem and multipotential progenitor cells was not because of alterations in hypoxia-inducible factor 1\u03b1 signaling or the oxygenation status of the bone marrow but was the consequence of the activation of the common \u03b2 subunit of the granulocyte-macrophage colony-stimulating factor\/interleukin-3 receptor driving glycolytic substrate utilization by mitochondria. By transplanting bone marrow from WT,\n              <jats:italic>Glut1<\/jats:italic>\n              <jats:sup>+\/\u2212<\/jats:sup>\n              ,\n              <jats:italic>ApoE<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              , and\n              <jats:italic>ApoE<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              <jats:italic>Glut1<\/jats:italic>\n              <jats:sup>+\/\u2212<\/jats:sup>\n              mice into hypercholesterolemic ApoE-deficient mice, we found that Glut1 deficiency reversed\n              <jats:italic>ApoE<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              hematopoietic stem and multipotential progenitor cell proliferation and expansion, which prevented the myelopoiesis and accelerated atherosclerosis of\n              <jats:italic>ApoE<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice transplanted with\n              <jats:italic>ApoE<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              bone marrow and resulted in reduced glucose uptake in the spleen and aortic arch of these mice.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Conclusions:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              We identified that Glut1 connects the enhanced glucose uptake in atheromatous plaques of\n              <jats:italic>ApoE<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice with their myelopoiesis through regulation of hematopoietic stem and multipotential progenitor cell maintenance and myelomonocytic fate and suggests Glut1 as potential drug target for atherosclerosis.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circresaha.115.307599","type":"journal-article","created":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T04:13:51Z","timestamp":1456805631000},"page":"1062-1077","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":121,"title":["Disruption of Glut1 in Hematopoietic Stem Cells Prevents Myelopoiesis and Enhanced Glucose Flux in Atheromatous Plaques of\n            <i>ApoE<\/i>\n            <sup>\u2212\/\u2212<\/sup>\n            Mice"],"prefix":"10.1161","volume":"118","author":[{"given":"Vincent","family":"Sarrazy","sequence":"first","affiliation":[{"name":"From the Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1065, Centre M\u00e9diterran\u00e9en de M\u00e9decine Mol\u00e9culaire (C3M), Nice, France (V.S., M.V., S.I., S.G.-P., R.G., L.Y.-C.); Division of Molecular Medicine, Department of Medicine (M.W.) and Department of Neurology (D.C.D.V.), Columbia University, New York, NY; Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) UMR_S 1166, H\u00f4pital de la Piti\u00e9, Paris, France (E.L.G.); Pierre &amp; Marie Curie University, Universit\u00e9..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manon","family":"Viaud","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1065, Centre M\u00e9diterran\u00e9en de M\u00e9decine Mol\u00e9culaire (C3M), Nice, France (V.S., M.V., S.I., S.G.-P., R.G., L.Y.-C.); Division of Molecular Medicine, Department of Medicine (M.W.) and Department of Neurology (D.C.D.V.), Columbia University, New York, NY; Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) UMR_S 1166, H\u00f4pital de la Piti\u00e9, Paris, France (E.L.G.); Pierre &amp; Marie Curie University, Universit\u00e9..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marit","family":"Westerterp","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1065, Centre M\u00e9diterran\u00e9en de M\u00e9decine Mol\u00e9culaire (C3M), Nice, France (V.S., M.V., S.I., S.G.-P., R.G., L.Y.-C.); Division of Molecular Medicine, Department of Medicine (M.W.) and Department of Neurology (D.C.D.V.), Columbia University, New York, NY; Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) UMR_S 1166, H\u00f4pital de la Piti\u00e9, Paris, France (E.L.G.); Pierre &amp; Marie Curie University, Universit\u00e9..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stoyan","family":"Ivanov","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1065, Centre M\u00e9diterran\u00e9en de M\u00e9decine Mol\u00e9culaire (C3M), Nice, France (V.S., M.V., S.I., S.G.-P., R.G., L.Y.-C.); Division of Molecular Medicine, Department of Medicine (M.W.) and Department of Neurology (D.C.D.V.), Columbia University, New York, NY; Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) UMR_S 1166, H\u00f4pital de la Piti\u00e9, Paris, France (E.L.G.); Pierre &amp; Marie Curie University, Universit\u00e9..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Giorgetti-Peraldi","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1065, Centre M\u00e9diterran\u00e9en de M\u00e9decine Mol\u00e9culaire (C3M), Nice, France (V.S., M.V., S.I., S.G.-P., R.G., L.Y.-C.); Division of Molecular Medicine, Department of Medicine (M.W.) and Department of Neurology (D.C.D.V.), Columbia University, New York, NY; Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) UMR_S 1166, H\u00f4pital de la Piti\u00e9, Paris, France (E.L.G.); Pierre &amp; Marie Curie University, Universit\u00e9..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolphe","family":"Guinamard","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1065, Centre M\u00e9diterran\u00e9en de M\u00e9decine Mol\u00e9culaire (C3M), Nice, France (V.S., M.V., S.I., S.G.-P., R.G., L.Y.-C.); Division of Molecular Medicine, Department of Medicine (M.W.) and Department of Neurology (D.C.D.V.), Columbia University, New York, NY; Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) UMR_S 1166, H\u00f4pital de la Piti\u00e9, Paris, France (E.L.G.); Pierre &amp; Marie Curie University, Universit\u00e9..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel L.","family":"Gautier","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1065, Centre M\u00e9diterran\u00e9en de M\u00e9decine Mol\u00e9culaire (C3M), Nice, France (V.S., M.V., S.I., S.G.-P., R.G., L.Y.-C.); Division of Molecular Medicine, Department of Medicine (M.W.) and Department of Neurology (D.C.D.V.), Columbia University, New York, NY; Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) UMR_S 1166, H\u00f4pital de la Piti\u00e9, Paris, France (E.L.G.); Pierre &amp; Marie Curie University, Universit\u00e9..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward B.","family":"Thorp","sequence":"additional","affiliation":[{"name":"From the Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U1065, Centre M\u00e9diterran\u00e9en de M\u00e9decine Mol\u00e9culaire (C3M), Nice, France (V.S., M.V., S.I., S.G.-P., R.G., L.Y.-C.); Division of Molecular Medicine, Department of Medicine (M.W.) and Department of Neurology 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