{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T21:50:56Z","timestamp":1792705856575,"version":"4.5.0"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2017,4,14]]},"abstract":"<jats:sec>\n                    <jats:title>\n                      <jats:underline>Rationale:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>Conventional 3-dimensional (3D) printing techniques cannot produce structures of the size at which individual cells interact.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>\n                      <jats:underline>Objective:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>Here, we used multiphoton-excited 3D printing to generate a native-like extracellular matrix scaffold with submicron resolution and then seeded the scaffold with cardiomyocytes, smooth muscle cells, and endothelial cells that had been differentiated from human-induced pluripotent stem cells to generate a human-induced pluripotent stem cell\u2013derived cardiac muscle patch (hCMP), which was subsequently evaluated in a murine model of myocardial infarction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>\n                      <jats:underline>Methods and Results:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>The scaffold was seeded with \u224850\u2009000 human-induced pluripotent stem cell\u2013derived cardiomyocytes, smooth muscle cells, and endothelial cells (in a 2:1:1 ratio) to generate the hCMP, which began generating calcium transients and beating synchronously within 1 day of seeding; the speeds of contraction and relaxation and the peak amplitudes of the calcium transients increased significantly over the next 7 days. When tested in mice with surgically induced myocardial infarction, measurements of cardiac function, infarct size, apoptosis, both vascular and arteriole density, and cell proliferation at week 4 after treatment were significantly better in animals treated with the hCMPs than in animals treated with cell-free scaffolds, and the rate of cell engraftment in hCMP-treated animals was 24.5% at week 1 and 11.2% at week 4.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>\n                      <jats:underline>Conclusions:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>Thus, the novel multiphoton-excited 3D printing technique produces extracellular matrix\u2013based scaffolds with exceptional resolution and fidelity, and hCMPs fabricated with these scaffolds may significantly improve recovery from ischemic myocardial injury.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/circresaha.116.310277","type":"journal-article","created":{"date-parts":[[2017,1,9]],"date-time":"2017-01-09T22:00:15Z","timestamp":1483999215000},"page":"1318-1325","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":295,"title":["Myocardial Tissue Engineering With Cells Derived From Human-Induced Pluripotent Stem Cells and a Native-Like, High-Resolution, 3-Dimensionally Printed Scaffold"],"prefix":"10.1161","volume":"120","author":[{"given":"Ling","family":"Gao","sequence":"first","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly E.","family":"Kupfer","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jangwook P.","family":"Jung","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libang","family":"Yang","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Da Sie","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quyen","family":"Tran","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Visar","family":"Ajeti","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian T.","family":"Freeman","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir G.","family":"Fast","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Campagnola","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda M.","family":"Ogle","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham (L.G., V.G.F., J.Z.); Department of Biomedical Engineering, University of Minnesota, Twin Cities, Minneapolis (M.E.K., J.P.J., L.Y., P.Z., B.T.F., B.M.O.); and Department of Biomedical Engineering, University of Wisconsin, Madison (Y.D.S., Q.T., V.A., P.J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00019.2004"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.901405"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2015.06.012"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.114.014998"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2016.09.024"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1394"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.306985"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.05.005"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1364\/OL.22.000132"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp048525r"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0507892"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0sm00140f"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.12.019"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmat3101"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2958"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1364\/OE.14.008613"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCHEARTFAILURE.114.001317"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1364\/OE.21.025346"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.03.064"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00155.2006"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2014.03.009"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.269894"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2006-0168"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.01.037"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1089\/ten.TEA.2012.0316"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1109\/IEMBS.2009.5333176"}],"container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCRESAHA.116.310277","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T23:53:32Z","timestamp":1715558012000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCRESAHA.116.310277"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,14]]},"references-count":26,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,4,14]]}},"alternative-id":["10.1161\/CIRCRESAHA.116.310277"],"URL":"https:\/\/doi.org\/10.1161\/circresaha.116.310277","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,14]]},"assertion":[{"value":"2016-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-01-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-01-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}