{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:19:31Z","timestamp":1790223571338,"version":"4.1.0"},"reference-count":24,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2018,7,20]]},"abstract":"\n \n Rationale:<\/jats:underline>\n <\/jats:title>\n Preclinical data in heart failure models suggest that repetitive stem cell therapy may be superior to single-dose cell administration.<\/jats:p>\n <\/jats:sec>\n \n \n Objective:<\/jats:underline>\n <\/jats:title>\n \n We investigated whether repetitive administration of CD34\n +<\/jats:sup>\n cells is superior to single-dose administration in patients with nonischemic dilated cardiomyopathy.\n <\/jats:p>\n <\/jats:sec>\n \n \n Methods and Results:<\/jats:underline>\n <\/jats:title>\n \n Of 66 patients with dilated cardiomyopathy, New York Heart Association functional class III, and left ventricular ejection fraction (LVEF) <40% enrolled in the study, 60 were randomly allocated to repetitive cell therapy (group A, n=30) or single-cell therapy (group B, n=30). Patients received G-CSF (granulocyte colony-stimulating factor) for 5 days, and 80 million CD34\n +<\/jats:sup>\n cells were collected by apheresis and injected transendocardially. In group A, cell therapy was repeated at 6 months. All patients were followed for 1 year, and the primary end point was the difference in change in LVEF between the groups. At baseline, the groups did not differ in age, sex, LVEF, NT-proBNP (N-terminal pro-B-type natriuretic peptide), or 6-minute walk test distance. When directly comparing groups A and B at 1 year, there was no significant difference in change in LVEF (from 32.2\u00b19.3% to 41.2\u00b16.5% in group A and from 30.0\u00b17.0% to 37.9\u00b15.3% in group B,\n P<\/jats:italic>\n =0.40). From baseline to 6 months, both groups improved in LVEF (+6.9\u00b13.3% in group A,\n P<\/jats:italic>\n =0.001 and +7.1\u00b13.5% in group B,\n P<\/jats:italic>\n =0.001), NT-proBNP (\u2212578\u00b1211 pg\/mL,\n P<\/jats:italic>\n =0.02 and \u2212633\u00b1305 pg\/mL,\n P<\/jats:italic>\n =0.01), and 6-minute walk test (+87\u00b121 m,\n P<\/jats:italic>\n =0.03 and +92\u00b125 m,\n P<\/jats:italic>\n =0.02). In contrast, we observed no significant changes between 6 months and 1 year (LVEF: +2.1\u00b12.3% in group A,\n P<\/jats:italic>\n =0.19 and +0.8\u00b13.1% in group B,\n P<\/jats:italic>\n =0.56; NT-proBNP: \u2212215\u00b1125 pg\/mL,\n P<\/jats:italic>\n =0.26 and \u221233\u00b1205 pg\/mL,\n P<\/jats:italic>\n =0.77; 6-minute walk test: +27\u00b111 m,\n P<\/jats:italic>\n =0.2 and +12\u00b118 m,\n P<\/jats:italic>\n =0.42).\n <\/jats:p>\n <\/jats:sec>\n \n \n Conclusions:<\/jats:underline>\n <\/jats:title>\n \n In patients with dilated cardiomyopathy, repetitive CD34\n +<\/jats:sup>\n cell administration does not seem to be associated with superior improvements in LVEF, NT-proBNP, or 6-minute walk test when compared with single-dose cell therapy.\n <\/jats:p>\n <\/jats:sec>\n \n \n Clinical Trial Registration:<\/jats:underline>\n <\/jats:title>\n \n URL:\n http:\/\/www.clinicaltrials.gov<\/jats:ext-link>\n . Unique identifier: NCT02248532.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/circresaha.117.312170","type":"journal-article","created":{"date-parts":[[2018,6,7]],"date-time":"2018-06-07T09:05:18Z","timestamp":1528362318000},"page":"389-396","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":35,"title":["Effects of Repetitive Transendocardial CD34\n +<\/sup>\n Cell Transplantation in Patients With Nonischemic Dilated Cardiomyopathy"],"prefix":"10.1161","volume":"123","author":[{"given":"Bojan","family":"Vrtovec","sequence":"first","affiliation":[{"name":"From the Advanced Heart Failure and Transplantation Center (B.V., G.P., G.Z., S.F., A.C., M.J.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregor","family":"Poglajen","sequence":"additional","affiliation":[{"name":"From the Advanced Heart Failure and Transplantation Center (B.V., G.P., G.Z., S.F., A.C., M.J.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matjaz","family":"Sever","sequence":"additional","affiliation":[{"name":"Department of Hematology (M.S., P.C.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregor","family":"Zemljic","sequence":"additional","affiliation":[{"name":"From the Advanced Heart Failure and Transplantation Center (B.V., G.P., G.Z., S.F., A.C., M.J.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabina","family":"Frljak","sequence":"additional","affiliation":[{"name":"From the Advanced Heart Failure and Transplantation Center (B.V., G.P., G.Z., S.F., A.C., M.J.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andraz","family":"Cerar","sequence":"additional","affiliation":[{"name":"From the Advanced Heart Failure and Transplantation Center (B.V., G.P., G.Z., S.F., A.C., M.J.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marko","family":"Cukjati","sequence":"additional","affiliation":[{"name":"UMC Ljubljana, Slovenia; National Blood Transfusion Institute, Ljubljana, Slovenia (M.C.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Jaklic","sequence":"additional","affiliation":[{"name":"From the Advanced Heart Failure and Transplantation Center (B.V., G.P., G.Z., S.F., A.C., M.J.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Cernelc","sequence":"additional","affiliation":[{"name":"Department of Hematology (M.S., P.C.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Haddad","sequence":"additional","affiliation":[{"name":"Stanford Cardiovascular Institute, Stanford University School of Medicine, CA (F.H., J.C.W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph C.","family":"Wu","sequence":"additional","affiliation":[{"name":"Stanford Cardiovascular Institute, Stanford University School of Medicine, CA (F.H., J.C.W.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2016.08.017"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehv390"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.112.000230"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.276519"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.117.312385"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCINTERVENTIONS.114.001436"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2011.64"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00059-007-3045-5"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehi162"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1994.tb08321.x"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2012.08.977"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/europace\/eup308"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurjhf\/hfp062"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1253\/circj.CJ-10-0898"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejheart.2008.05.010"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1159\/000363133"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/circinterventions.115.002740"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.14503\/THIJ-15-5341"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2016.08.002"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.308937"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-017-0606-5"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2016.05.008"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2016.11.009"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.16-0331"}],"container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCRESAHA.117.312170","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T04:51:55Z","timestamp":1715575915000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCRESAHA.117.312170"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,20]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,7,20]]}},"alternative-id":["10.1161\/CIRCRESAHA.117.312170"],"URL":"https:\/\/doi.org\/10.1161\/circresaha.117.312170","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,20]]},"assertion":[{"value":"2018-06-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}