{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:41:39Z","timestamp":1791308499765,"version":"4.3.1"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"7","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2018,3,30]]},"abstract":"<jats:sec>\n                    <jats:title>\n                      <jats:underline>Rationale:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>Endocardium is the major source of coronary endothelial cells (ECs) in the fetal and neonatal hearts. It remains unclear whether endocardium in the adult stage is also the main origin of neovascularization after cardiac injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>\n                      <jats:underline>Objective:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>To define the vascular potential of adult endocardium in homeostasis and after cardiac injuries by fate-mapping studies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>\n                      <jats:underline>Methods and Results:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>\n                      We generate an inducible adult endocardial Cre line (\n                      <jats:italic toggle=\"yes\">Npr3<\/jats:italic>\n                      [natriuretic peptide receptor C]-\n                      <jats:italic toggle=\"yes\">CreER<\/jats:italic>\n                      ) and show that\n                      <jats:italic toggle=\"yes\">Npr3-CreER<\/jats:italic>\n                      efficiently and specifically labels endocardial cells but not coronary blood vessels in the adult heart. The adult endocardial cells do not contribute to any vascular ECs during cardiac homeostasis. To examine the formation of blood vessels from endocardium after injury, we generate 4 cardiac injury models with\n                      <jats:italic toggle=\"yes\">Npr3-CreER<\/jats:italic>\n                      mice: myocardial infarction, myocardial ischemia\u2013reperfusion, cryoinjury, and transverse aortic constriction. Lineage tracing experiments show that adult endocardium minimally contributes to coronary ECs after myocardial infarction. In the myocardial ischemia\u2013reperfusion, cryoinjury, or transverse aortic constriction models, adult endocardial cells do not give rise to any vascular ECs, and they remain on the inner surface of myocardium that connects with lumen circulation. In the myocardial infarction model, very few endocardial cells are trapped in the infarct zone of myocardium shortly after ligation of coronary artery, indicating the involvement of endocardial entrapment during blood vessels formation. When these adult endocardial cells are relocated and trapped in the infarcted myocardium by transplantation or myocardial constriction model, very few endocardial cells survive and gain vascular EC properties, and their contribution to neovascularization in the injured myocardium remains minimal.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>\n                      <jats:underline>Conclusions:<\/jats:underline>\n                    <\/jats:title>\n                    <jats:p>Unlike its fetal or neonatal counterpart, adult endocardium naturally generates minimal, if any, coronary arteries or vascular ECs during cardiac homeostasis or after injuries.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/circresaha.117.312354","type":"journal-article","created":{"date-parts":[[2018,1,26]],"date-time":"2018-01-26T05:27:27Z","timestamp":1516944447000},"page":"984-993","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":82,"title":["Genetic Fate Mapping Defines the Vascular Potential of Endocardial Cells in the Adult Heart"],"prefix":"10.1161","volume":"122","author":[{"given":"Juan","family":"Tang","sequence":"first","affiliation":[{"name":"From the State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.) and Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.), Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China; School of Life Science and Technology, ShanghaiTech University, China (H.Z., B.Z.); Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China (D.C., B.Z.); and Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR, China (K.O.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.) and Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.), Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China; School of Life Science and Technology, ShanghaiTech University, China (H.Z., B.Z.); Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China (D.C., B.Z.); and Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR, China (K.O.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingjuan","family":"He","sequence":"additional","affiliation":[{"name":"From the State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.) and Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.), Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China; School of Life Science and Technology, ShanghaiTech University, China (H.Z., B.Z.); Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China (D.C., B.Z.); and Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR, China (K.O.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuzhen","family":"Huang","sequence":"additional","affiliation":[{"name":"From the State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.) and Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.), Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China; School of Life Science and Technology, ShanghaiTech University, China (H.Z., B.Z.); Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China (D.C., B.Z.); and Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR, China (K.O.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"From the State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.) and Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.), Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China; School of Life Science and Technology, ShanghaiTech University, China (H.Z., B.Z.); Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China (D.C., B.Z.); and Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR, China (K.O.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjuan","family":"Pu","sequence":"additional","affiliation":[{"name":"From the State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.) and Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.), Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China; School of Life Science and Technology, ShanghaiTech University, China (H.Z., B.Z.); Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China (D.C., B.Z.); and Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR, China (K.O.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Yu","sequence":"additional","affiliation":[{"name":"From the State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.) and Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.), Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China; School of Life Science and Technology, ShanghaiTech University, China (H.Z., B.Z.); Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China (D.C., B.Z.); and Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR, China (K.O.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Libo","family":"Zhang","sequence":"additional","affiliation":[{"name":"From the State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.) and Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.), Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China; School of Life Science and Technology, ShanghaiTech University, China (H.Z., B.Z.); Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China (D.C., B.Z.); and Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR, China (K.O.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongqing","family":"Cai","sequence":"additional","affiliation":[{"name":"From the State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.) and Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.), Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China; School of Life Science and Technology, ShanghaiTech University, China (H.Z., B.Z.); Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China (D.C., B.Z.); and Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR, China (K.O.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy O.","family":"Lui","sequence":"additional","affiliation":[{"name":"From the State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.) and Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.), Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China; School of Life Science and Technology, ShanghaiTech University, China (H.Z., B.Z.); Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China (D.C., B.Z.); and Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR, China (K.O.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zhou","sequence":"additional","affiliation":[{"name":"From the State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Institute of Biochemistry and Cell Biology (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.) and Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences (J.T., H.Z., L.H., X.H., Y.L., W.P., W.Y., L.Z., B.Z.), Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, China; School of Life Science and Technology, ShanghaiTech University, China (H.Z., B.Z.); Key Laboratory of Regenerative Medicine of Ministry of Education, Institute of Aging and Regenerative Medicine, Jinan University, Guangzhou, China (D.C., B.Z.); and Department of Chemical Pathology, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, SAR, China (K.O.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04478"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(03)00258-X"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvr035"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.305373"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08873"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2013.83"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.10.023"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0706"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.01.012"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1251487"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.113639"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.308749"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07060"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1509834113"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092430110"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.20578"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI45529"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI74783"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13839"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.306476"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12422"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.303794"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI93868"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2467"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.305097"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7020"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.116.024754"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.305350"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2006.12.011"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.307202"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.06.004"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(20000401)258:4<319::AID-AR1>3.0.CO;2-O"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00118-1"}],"container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCRESAHA.117.312354","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T16:34:27Z","timestamp":1783528467000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCRESAHA.117.312354"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,30]]},"references-count":33,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2018,3,30]]}},"alternative-id":["10.1161\/CIRCRESAHA.117.312354"],"URL":"https:\/\/doi.org\/10.1161\/circresaha.117.312354","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,30]]},"assertion":[{"value":"2017-11-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-22","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}