{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T15:07:36Z","timestamp":1781363256342,"version":"3.54.1"},"reference-count":42,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2019,9,27]]},"abstract":"<jats:sec>\n            <jats:title>\n              <jats:underline>Rationale:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>Human umbilical cord blood (hUCB) contains diverse populations of stem\/progenitor cells. Whether hUCB-derived nonhematopoietic cells would induce cardiac repair remains unknown.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Objective:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              To examine whether intramyocardial transplantation of hUCB-derived CD45\n              <jats:sup>\u2212<\/jats:sup>\n              Lin\n              <jats:sup>\u2212<\/jats:sup>\n              nonhematopoietic cellular fraction after a reperfused myocardial infarction in nonimmunosuppressed rats would improve cardiac function and ameliorate ventricular remodeling.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Methods and Results:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              Nonhematopoietic CD45\n              <jats:sup>\u2212<\/jats:sup>\n              Lin\n              <jats:sup>\u2212<\/jats:sup>\n              cells were isolated from hUCB. Flow cytometry and quantitative polymerase chain reaction were used to characterize this subpopulation. Age-matched male Fischer 344 rats underwent a 30-minute coronary occlusion followed by reperfusion and 48 hours later received intramyocardial injection of vehicle or hUCB CD45\n              <jats:sup>\u2212<\/jats:sup>\n              Lin\n              <jats:sup>\u2212<\/jats:sup>\n              cells. After 35 days, compared with vehicle-treated rats, CD45\n              <jats:sup>\u2212<\/jats:sup>\n              Lin\n              <jats:sup>\u2212<\/jats:sup>\n              cell\u2013treated rats exhibited improved left ventricular function, blunted left ventricular hypertrophy, greater preservation of viable myocardium in the infarct zone, and superior left ventricular remodeling. Mechanistically, hUCB CD45\n              <jats:sup>\u2212<\/jats:sup>\n              Lin\n              <jats:sup>\u2212<\/jats:sup>\n              cell injection favorably modulated molecular pathways regulating myocardial fibrosis, cardiomyocyte apoptosis, angiogenesis, and inflammation in postinfarct ventricular myocardium. Rare persistent transplanted human cells could be detected at both 4 and 35 days after myocardial infarction.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>\n              <jats:underline>Conclusions:<\/jats:underline>\n            <\/jats:title>\n            <jats:p>\n              Transplantation of hUCB-derived CD45\n              <jats:sup>\u2212<\/jats:sup>\n              Lin\n              <jats:sup>\u2212<\/jats:sup>\n              nonhematopoietic cellular subfraction after a reperfused myocardial infarction in nonimmunosuppressed rats ameliorates left ventricular dysfunction and improves remodeling via favorable paracrine modulation of molecular pathways. These findings with human cells in a clinically relevant model of myocardial ischemia\/reperfusion in immunocompetent animals may have significant translational implications.Visual Overview: An online visual overview is available for this article.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circresaha.119.315216","type":"journal-article","created":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T09:01:34Z","timestamp":1567069294000},"page":"759-772","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":13,"title":["Transplantation of Human Umbilical Cord Blood\u2013Derived Cellular Fraction Improves Left Ventricular Function and Remodeling After Myocardial Ischemia\/Reperfusion"],"prefix":"10.1161","volume":"125","author":[{"given":"Lin","family":"Zhao","sequence":"first","affiliation":[{"name":"From the Department of Internal Medicine, University of Nevada, Las Vegas School of Medicine (L.Z., G.C., M.G., J.H., Y.Y., B.D.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangming","family":"Cheng","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine, University of Nevada, Las Vegas School of Medicine (L.Z., G.C., M.G., J.H., Y.Y., B.D.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kashyap","family":"Choksi","sequence":"additional","affiliation":[{"name":"Cardiology Consultants of South Georgia, Thomasville (K.C.)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anweshan","family":"Samanta","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine (A.S.), University of Missouri-Kansas City"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdy","family":"Girgis","sequence":"additional","affiliation":[{"name":"From the Department of Internal Medicine, University of Nevada, Las Vegas School of Medicine (L.Z., G.C., M.G., J.H., Y.Y., 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