{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:17:10Z","timestamp":1792016230414,"version":"4.4.0"},"reference-count":82,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","funder":[{"DOI":"10.13039\/501100001674","name":"Fondation Leducq","doi-asserted-by":"publisher","award":["SphingoNet"],"award-info":[{"award-number":["SphingoNet"]}],"id":[{"id":"10.13039\/501100001674","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["DCP20171138945-EC"],"award-info":[{"award-number":["DCP20171138945-EC"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-19-CE14-0028-01"],"award-info":[{"award-number":["ANR-19-CE14-0028-01"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004431","name":"Fondation de France","doi-asserted-by":"publisher","award":["Cardiovascular"],"award-info":[{"award-number":["Cardiovascular"]}],"id":[{"id":"10.13039\/501100004431","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007354","name":"Fondation Lefoulon Delalande","doi-asserted-by":"publisher","award":["Bourse de Recherche\/Anja Nitzsche"],"award-info":[{"award-number":["Bourse de Recherche\/Anja Nitzsche"]}],"id":[{"id":"10.13039\/100007354","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["Intramural Research Program"],"award-info":[{"award-number":["Intramural Research Program"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2021,2,5]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale:<\/jats:title>\n                    <jats:p>\n                      Cerebrovascular function is critical for brain health, and endogenous vascular protective pathways may provide therapeutic targets for neurological disorders. S1P (Sphingosine 1-phosphate) signaling coordinates vascular functions in other organs, and S1P\n                      <jats:sub>1<\/jats:sub>\n                      (S1P receptor-1) modulators including fingolimod show promise for the treatment of ischemic and hemorrhagic stroke. However, S1P\n                      <jats:sub>1<\/jats:sub>\n                      also coordinates lymphocyte trafficking, and lymphocytes are currently viewed as the principal therapeutic target for S1P\n                      <jats:sub>1<\/jats:sub>\n                      modulation in stroke.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective:<\/jats:title>\n                    <jats:p>\n                      To address roles and mechanisms of engagement of endothelial cell S1P\n                      <jats:sub>1<\/jats:sub>\n                      in the naive and ischemic brain and its potential as a target for cerebrovascular therapy.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results:<\/jats:title>\n                    <jats:p>\n                      Using spatial modulation of S1P provision and signaling, we demonstrate a critical vascular protective role for endothelial S1P\n                      <jats:sub>1<\/jats:sub>\n                      in the mouse brain. With an S1P\n                      <jats:sub>1<\/jats:sub>\n                      signaling reporter, we reveal that abluminal polarization shields S1P\n                      <jats:sub>1<\/jats:sub>\n                      from circulating endogenous and synthetic ligands after maturation of the blood-neural barrier, restricting homeostatic signaling to a subset of arteriolar endothelial cells. S1P\n                      <jats:sub>1<\/jats:sub>\n                      signaling sustains hallmark endothelial functions in the naive brain and expands during ischemia by engagement of cell-autonomous S1P provision. Disrupting this pathway by endothelial cell-selective deficiency in S1P production, export, or the S1P\n                      <jats:sub>1<\/jats:sub>\n                      receptor substantially exacerbates brain injury in permanent and transient models of ischemic stroke. By contrast, profound lymphopenia induced by loss of lymphocyte S1P\n                      <jats:sub>1<\/jats:sub>\n                      provides modest protection only in the context of reperfusion. In the ischemic brain, endothelial cell S1P\n                      <jats:sub>1<\/jats:sub>\n                      supports blood-brain barrier function, microvascular patency, and the rerouting of blood to hypoperfused brain tissue through collateral anastomoses. Boosting these functions by supplemental pharmacological engagement of the endothelial receptor pool with a blood-brain barrier penetrating S1P\n                      <jats:sub>1<\/jats:sub>\n                      -selective agonist can further reduce cortical infarct expansion in a therapeutically relevant time frame and independent of reperfusion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>\n                      This study provides genetic evidence to support a pivotal role for the endothelium in maintaining perfusion and microvascular patency in the ischemic penumbra that is coordinated by S1P signaling and can be harnessed for neuroprotection with blood-brain barrier-penetrating S1P\n                      <jats:sub>1<\/jats:sub>\n                      agonists.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/circresaha.120.316711","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T05:00:13Z","timestamp":1606885213000},"page":"363-382","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":135,"title":["Endothelial S1P\n                    <sub>1<\/sub>\n                    Signaling Counteracts Infarct Expansion in Ischemic Stroke"],"prefix":"10.1161","volume":"128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0567-6790","authenticated-orcid":true,"given":"Anja","family":"Nitzsche","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marine","family":"Poittevin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."},{"name":"Institut des Vaisseaux et du Sang, H\u00f4pital Lariboisi\u00e8re, France (M.P., P.-L.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ammar","family":"Benarab","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3184-3740","authenticated-orcid":true,"given":"Philippe","family":"Bonnin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, INSERM U965 and Physiologie Clinique - Explorations-Fonctionnelles, AP-HP, H\u00f4pital Lariboisi\u00e8re, France (P.B., N.K.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8675-4580","authenticated-orcid":true,"given":"Giuseppe","family":"Faraco","sequence":"additional","affiliation":[{"name":"Feil Family Brain and Mind Research Institute (G.F., L.G.-B., C.I.), Weill Cornell Medical College, Cornell University, New York."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Uchida","sequence":"additional","affiliation":[{"name":"Center for Vascular Biology (H.U., T.S.), Weill Cornell Medical College, Cornell University, New York."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Favre","sequence":"additional","affiliation":[{"name":"MITOVASC Institute, CARFI Facility, CNRS UMR 6015, INSERM U1083, Angers University, France (J.F., M.C.L.G., D.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidia","family":"Garcia-Bonilla","sequence":"additional","affiliation":[{"name":"Feil Family Brain and Mind Research Institute (G.F., L.G.-B., C.I.), Weill Cornell Medical College, Cornell University, New York."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela C.L.","family":"Garcia","sequence":"additional","affiliation":[{"name":"MITOVASC Institute, CARFI Facility, CNRS UMR 6015, INSERM U1083, Angers University, France (J.F., M.C.L.G., D.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Louis","family":"L\u00e9ger","sequence":"additional","affiliation":[{"name":"Institut des Vaisseaux et du Sang, H\u00f4pital Lariboisi\u00e8re, France (M.P., P.-L.L.)."},{"name":"INSERM U1141, H\u00f4pital Robert Debr\u00e9 (P.-L.L., C.C.-M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Th\u00e9rond","sequence":"additional","affiliation":[{"name":"Assistance Publique-H\u00f4pitaux de Paris (AP-HP), Service de Biochimie, H\u00f4pital de Bic\u00eatre, Le Kremlin Bic\u00eatre, France (P.T.)."},{"name":"Universit\u00e9 Paris-Sud, France (P.T.)."},{"name":"UFR de Pharmacie, EA 4529, Ch\u00e2tenay-Malabry, France (P.T.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7761-1684","authenticated-orcid":true,"given":"Thomas","family":"Mathivet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7335-350X","authenticated-orcid":true,"given":"Gwennhael","family":"Autret","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Baudrie","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludovic","family":"Couty","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2447-4350","authenticated-orcid":true,"given":"Mari","family":"Kono","sequence":"additional","affiliation":[{"name":"National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Institutes of Health, Bethesda, MD, USA (M.K., R.L.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline","family":"Chevallier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4737-2613","authenticated-orcid":true,"given":"Hira","family":"Niazi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6062-5905","authenticated-orcid":true,"given":"Pierre-Louis","family":"Tharaux","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerold","family":"Chun","sequence":"additional","affiliation":[{"name":"Neuroscience Drug Discovery, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA (J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan R.","family":"Schwab","sequence":"additional","affiliation":[{"name":"Skirball Institute of Biomolecular Medicine, New York University School of Medicine, NY (S.R.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Eichmann","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5349-8194","authenticated-orcid":true,"given":"Bertrand","family":"Tavitian","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard L.","family":"Proia","sequence":"additional","affiliation":[{"name":"National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Institutes of Health, Bethesda, MD, USA (M.K., R.L.P.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9632-5453","authenticated-orcid":true,"given":"Christiane","family":"Charriaut-Marlangue","sequence":"additional","affiliation":[{"name":"INSERM U1141, H\u00f4pital Robert Debr\u00e9 (P.-L.L., C.C.-M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2711-7423","authenticated-orcid":true,"given":"Teresa","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Center for Vascular Biology (H.U., T.S.), Weill Cornell Medical College, Cornell University, New York."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Kubis","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, INSERM U965 and Physiologie Clinique - Explorations-Fonctionnelles, AP-HP, H\u00f4pital Lariboisi\u00e8re, France (P.B., N.K.)."},{"name":"Universit\u00e9 de Paris, INSERM U1148, H\u00f4pital Bichat, France (N.K.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1094-0285","authenticated-orcid":true,"given":"Daniel","family":"Henrion","sequence":"additional","affiliation":[{"name":"MITOVASC Institute, CARFI Facility, CNRS UMR 6015, INSERM U1083, Angers University, France (J.F., M.C.L.G., D.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9797-073X","authenticated-orcid":true,"given":"Costantino","family":"Iadecola","sequence":"additional","affiliation":[{"name":"Feil Family Brain and Mind Research Institute (G.F., L.G.-B., C.I.), Weill Cornell Medical College, Cornell University, New York."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8355-4065","authenticated-orcid":true,"given":"Timothy","family":"Hla","sequence":"additional","affiliation":[{"name":"Vascular Biology Program, Boston Children\u2019s Hospital, MA (T.H.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6271-7125","authenticated-orcid":true,"given":"Eric","family":"Camerer","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Paris Cardiovascular Research Centre, INSERM U970, France (A.N., M.P., A.B., T.M., G.A., V.B., L.C., A.C., H.N., P.-L.T., A.E., B.T., E.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.07.002"},{"key":"e_1_3_6_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/STR.0000000000000158"},{"key":"e_1_3_6_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.113.003798"},{"key":"e_1_3_6_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.119.025353"},{"key":"e_1_3_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(11)70195-8"},{"key":"e_1_3_6_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/1756286418770626"},{"key":"e_1_3_6_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI76369"},{"key":"e_1_3_6_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-020711-075011"},{"key":"e_1_3_6_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.308929"},{"key":"e_1_3_6_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.117.09088"},{"key":"e_1_3_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.07.015"},{"key":"e_1_3_6_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aaa2581"},{"key":"e_1_3_6_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1139221"},{"key":"e_1_3_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature24053"},{"key":"e_1_3_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2009.08.142"},{"key":"e_1_3_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.22186"},{"key":"e_1_3_6_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.109.568899"},{"key":"e_1_3_6_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0138029"},{"key":"e_1_3_6_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.116.015371"},{"key":"e_1_3_6_21_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.113.002880"},{"key":"e_1_3_6_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.115.012236"},{"key":"e_1_3_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.25352"},{"key":"e_1_3_6_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.115.016371"},{"key":"e_1_3_6_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.119.027134"},{"key":"e_1_3_6_26_2","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2019031948"},{"key":"e_1_3_6_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1618659114"},{"key":"e_1_3_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2012.09.009"},{"key":"e_1_3_6_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-5620-3_3"},{"key":"e_1_3_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20367"},{"key":"e_1_3_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2005.07.002"},{"key":"e_1_3_6_32_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0899"},{"key":"e_1_3_6_33_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci38575"},{"key":"e_1_3_6_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8893"},{"key":"e_1_3_6_35_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7660125"},{"key":"e_1_3_6_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-015-9008-4"},{"key":"e_1_3_6_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature25739"},{"key":"e_1_3_6_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-019-0491-3"},{"key":"e_1_3_6_39_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI71194"},{"key":"e_1_3_6_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14462"},{"key":"e_1_3_6_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.03.003"},{"key":"e_1_3_6_42_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.114.004816"},{"key":"e_1_3_6_43_2","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.117.007804"},{"key":"e_1_3_6_44_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20112322"},{"key":"e_1_3_6_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-012-0294-0"},{"key":"e_1_3_6_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.547"},{"key":"e_1_3_6_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep02582"},{"key":"e_1_3_6_48_2","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.107.127183"},{"key":"e_1_3_6_49_2","doi-asserted-by":"publisher","DOI":"10.1124\/mol.111.076109"},{"key":"e_1_3_6_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2010.10"},{"key":"e_1_3_6_51_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12883-016-0722-0"},{"key":"e_1_3_6_52_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199609000-00023"},{"key":"e_1_3_6_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-017-0059-z"},{"key":"e_1_3_6_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1688-z"},{"key":"e_1_3_6_55_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.49405"},{"key":"e_1_3_6_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep24028"},{"key":"e_1_3_6_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1014154108"},{"key":"e_1_3_6_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05030-w"},{"key":"e_1_3_6_59_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI45403"},{"key":"e_1_3_6_60_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aal2722"},{"key":"e_1_3_6_61_2","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aax8050"},{"key":"e_1_3_6_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01340-7"},{"key":"e_1_3_6_63_2","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.108.543561"},{"key":"e_1_3_6_64_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260419"},{"key":"e_1_3_6_65_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000244088.33375.52"},{"key":"e_1_3_6_66_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1416166111"},{"key":"e_1_3_6_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2020.135160"},{"key":"e_1_3_6_68_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1973910"},{"key":"e_1_3_6_69_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20335"},{"key":"e_1_3_6_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2011.02.030"},{"key":"e_1_3_6_71_2","doi-asserted-by":"publisher","DOI":"10.2337\/db14-0759"},{"key":"e_1_3_6_72_2","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.113.01761"},{"key":"e_1_3_6_73_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2980-15.2015"},{"key":"e_1_3_6_74_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-328-8_13"},{"key":"e_1_3_6_75_2","doi-asserted-by":"publisher","DOI":"10.1159\/000441663"},{"key":"e_1_3_6_76_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701109"},{"key":"e_1_3_6_77_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1002125"},{"key":"e_1_3_6_78_2","doi-asserted-by":"publisher","DOI":"10.2144\/000112257"},{"key":"e_1_3_6_79_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-019-0212-0"},{"key":"e_1_3_6_80_2","first-page":"e52204","article-title":"Isolation of primary murine brain microvascular endothelial cells.","author":"Ruck T","year":"2014","unstructured":"Ruck, T, Bittner, S, Epping, L, Herrmann, AM, Meuth, SG. Isolation of primary murine brain microvascular endothelial cells. J Vis Exp. 2014;93:e52204.","journal-title":"J Vis Exp"},{"key":"e_1_3_6_81_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.08.040"},{"key":"e_1_3_6_82_2","doi-asserted-by":"publisher","DOI":"10.21769\/BioProtoc.2294"},{"key":"e_1_3_6_83_2","doi-asserted-by":"publisher","DOI":"10.1038\/sdata.2018.160"}],"container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCRESAHA.120.316711","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T13:39:06Z","timestamp":1780061946000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCRESAHA.120.316711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2,5]]},"references-count":82,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,2,5]]}},"alternative-id":["10.1161\/CIRCRESAHA.120.316711"],"URL":"https:\/\/doi.org\/10.1161\/circresaha.120.316711","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,2,5]]},"assertion":[{"value":"2020-01-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-12","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}