{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:29:53Z","timestamp":1790314193923,"version":"4.1.0"},"reference-count":18,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","license":[{"start":{"date-parts":[[2025,5,9]],"date-time":"2025-05-09T00:00:00Z","timestamp":1746748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2025,5,9]]},"abstract":"<jats:sec>\n            <jats:title>BACKGROUND:<\/jats:title>\n            <jats:p>Mouse models of myocardial ischemia with subsequent heart failure are common approaches to examine heart failure pathology and possible treatment strategies. We sought to establish a high-throughput approach for echocardiography-guided induction of myocardial ischemia\/reperfusion (IR) in mice.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>METHODS:<\/jats:title>\n            <jats:p>After visualization of the left coronary artery with high-resolution ultrasound imaging and echocardiographic definition of the level of coronary occlusion, the left anterior descending artery was temporarily occluded with 2 micromanipulator-controlled needles. Functional and molecular changes were assessed and compared with commonly performed surgical techniques.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>RESULTS:<\/jats:title>\n            <jats:p>Echocardiography-guided induction of myocardial IR enabled standardized induction of myocardial IR injury with subsequent left ventricular remodeling. Incorporation of various quality control measures throughout the procedure ensured a high success rate and the absence of relevant postinterventional mortality in experienced hands. Compared with surgical approaches, echocardiography-guided induction of myocardial IR showed a quicker recovery time and induced a less pronounced inflammatory response characterized by decreased local and systemic neutrophil counts. Notably, infarct size and subsequent post\u2013myocardial infarction cardiac dysfunction were comparable between methods. The novel procedure was successfully implemented at different academic institutions with imaging expertise and demonstrated high interinstitutional reproducibility.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>CONCLUSIONS:<\/jats:title>\n            <jats:p>Echocardiography-guided induction of myocardial IR enables high-throughput induction of myocardial IR injury with precise echocardiographic definition of the occlusion level and immediate evaluation of cardiac function during ischemia. The method provides a more clinically relevant assessment of IR sequelae and offers notable animal welfare advantages by eliminating the need for ventilation and thoracotomy, thereby mitigating potential surgery-related confounders.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circresaha.125.326156","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T09:00:18Z","timestamp":1744275618000},"page":"1099-1109","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":14,"title":["High-Throughput Echocardiography-Guided Induction of Myocardial Ischemia\/Reperfusion in Mice"],"prefix":"10.1161","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6707-1695","authenticated-orcid":true,"given":"Florian","family":"Sicklinger","sequence":"first","affiliation":[{"name":"Department of Internal Medicine III, University Hospital Heidelberg, Germany (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."},{"name":"German Centre for Cardiovascular Research (DZHK), Heidelberg (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklas","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, University Hospital Heidelberg, Germany (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."},{"name":"German Centre for Cardiovascular Research (DZHK), Heidelberg (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."},{"name":"Cardiovascular Division, Department of Medicine, Center for Cardiovascular Research, Washington University School of Medicine, St. Louis (N.H., A.K., C.W., J.N., J.M.A., S.D., K.J.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila","family":"Kovacs","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, Department of Medicine, Center for Cardiovascular Research, Washington University School of Medicine, St. Louis (N.H., A.K., C.W., J.N., J.M.A., S.D., K.J.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7922-7512","authenticated-orcid":true,"given":"Carla","family":"Weinheimer","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, Department of Medicine, Center for Cardiovascular Research, Washington University School of Medicine, St. Louis (N.H., A.K., C.W., J.N., J.M.A., S.D., K.J.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jess","family":"Nigro","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, Department of Medicine, Center for Cardiovascular Research, Washington University School of Medicine, St. Louis (N.H., A.K., C.W., J.N., J.M.A., S.D., K.J.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7284-2127","authenticated-orcid":true,"given":"Tobias","family":"Thiemann","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, University Hospital Heidelberg, Germany (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."},{"name":"German Centre for Cardiovascular Research (DZHK), Heidelberg (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6851-0168","authenticated-orcid":true,"given":"Junedh M.","family":"Amrute","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, Department of Medicine, Center for Cardiovascular Research, Washington University School of Medicine, St. Louis (N.H., A.K., C.W., J.N., J.M.A., S.D., K.J.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2308-6543","authenticated-orcid":true,"given":"David","family":"Schumacher","sequence":"additional","affiliation":[{"name":"Department of Medicine II (Nephrology, Rheumatology, Clinical Immunology and Hypertension) (D.S., R.K.), Faculty of Medicine, RWTH Aachen University, Germany."},{"name":"Department of Anesthesiology (D.S.), Faculty of Medicine, RWTH Aachen University, Germany."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moritz P.","family":"Kornadt","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, University Hospital Heidelberg, Germany (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."},{"name":"German Centre for Cardiovascular Research (DZHK), Heidelberg (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7587-8422","authenticated-orcid":true,"given":"Laura M.","family":"Wienecke","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, University Hospital Heidelberg, Germany (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."},{"name":"German Centre for Cardiovascular Research (DZHK), Heidelberg (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennart","family":"Rompel","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, University Hospital Heidelberg, Germany (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."},{"name":"German Centre for Cardiovascular Research (DZHK), Heidelberg (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4327-1079","authenticated-orcid":true,"given":"Johannes","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, University Hospital Heidelberg, Germany (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."},{"name":"German Centre for Cardiovascular Research (DZHK), Heidelberg (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2912-9911","authenticated-orcid":true,"given":"John","family":"Bachman","sequence":"additional","affiliation":[{"name":"The Jackson Laboratory, Bar Harbor, ME (J.B., O.B., 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Cardiovascular Research (DZHK), Heidelberg (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2344-1856","authenticated-orcid":true,"given":"Mirko","family":"V\u00f6lkers","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine III, University Hospital Heidelberg, Germany (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."},{"name":"German Centre for Cardiovascular Research (DZHK), Heidelberg (F.S., N.H., T.T., M.P.K., L.M.W., L.R., J.F., T.C.K., M.V., N.F., F.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8035-0048","authenticated-orcid":true,"given":"Ralf P.","family":"Brandes","sequence":"additional","affiliation":[{"name":"Institute for Cardiovascular Physiology, Goethe University Frankfurt, Germany (R.P.B.)."},{"name":"German Center for Cardiovascular Research 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