{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:31:17Z","timestamp":1791318677993,"version":"4.3.1"},"reference-count":49,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2008,2,12]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Background\u2014<\/jats:italic>\n            <\/jats:bold>\n            The pathophysiology of infective endocarditis involves a pathogen\/host tissue interaction, leading to formation of infected thrombotic vegetations. Annexin V is a ligand of phosphatidylserines exposed by activated platelets and apoptotic cells. Because vegetations are platelet-fibrin clots in which platelet proaggregant activity is enhanced by bacterial colonization, we investigated the ability of annexin V labeled with technetium Tc 99m (\n            <jats:sup>99m<\/jats:sup>\n            Tc-ANX) to provide functional imaging of these vegetations in experimental models of infective endocarditis. This ability was assessed in rabbits and rats because of the different interest of these 2 species in preclinical analysis.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            Nonbacterial thrombotic endocarditis was induced with the use of a catheter left indwelling through the aortic or tricuspid valve, and animals were injected with either a bacterial inoculum or saline. Scintigraphic investigations were performed 5 days later and showed a higher\n            <jats:sup>99m<\/jats:sup>\n            Tc-ANX uptake by vegetations in infected versus noninfected animals (ratio, 1.3 for in vivo acquisitions and 2 for autoradiography;\n            <jats:italic>P<\/jats:italic>\n            &lt;0.0001 for all), whereas no significant uptake was present in controls. Right-sided endocarditis was associated with pulmonary uptake foci corresponding to emboli. Histological analysis of vegetations showed a specific uptake of\n            <jats:sup>99m<\/jats:sup>\n            Tc-ANX at the interface between circulating blood and vegetation. In parallel, underlying myocardial tissue showed myocyte apoptosis and mucoid degeneration, without extracellular matrix degradation at this stage.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            <jats:sup>99m<\/jats:sup>\n            Tc-ANX is suitable for functional imaging of platelet-fibrin vegetations in endocarditis, as well as embolic events.\n            <jats:sup>99m<\/jats:sup>\n            Tc-ANX uptake reflects mainly platelet activation in the luminal layer of vegetations. This uptake is enhanced by bacterial colonization.\n          <\/jats:p>","DOI":"10.1161\/circulationaha.107.718114","type":"journal-article","created":{"date-parts":[[2008,1,29]],"date-time":"2008-01-29T02:11:22Z","timestamp":1201572682000},"page":"781-789","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":44,"title":["Technetium 99m\u2013Labeled Annexin V Scintigraphy of Platelet Activation in Vegetations of Experimental Endocarditis"],"prefix":"10.1161","volume":"117","author":[{"given":"Francois","family":"Rouzet","sequence":"first","affiliation":[{"name":"From AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Service de Me\u0301decine Nucle\u0301aire; Universite\u0301 Denis Diderot\u2013Paris 7, UMR S773; and INSERM U773, Paris, France (F.R., L.S.-M., D.L.G.); INSERM U698, Cardiovascular Remodeling, Paris 7 University, CHU Xavier Bichat, Paris, France (M.D.H., L.L., J.B.M.); CEA-DRM-SHFJ, Orsay, France (F.H.); EA3964, Universite\u0301 Denis Diderot\u2013Paris 7, Paris, France (A.L., B.F); INSERM CIC 007; AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Centre d\u2019Investigation..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel Dominguez","family":"Hernandez","sequence":"additional","affiliation":[{"name":"From AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Service de Me\u0301decine Nucle\u0301aire; Universite\u0301 Denis Diderot\u2013Paris 7, UMR S773; and INSERM U773, Paris, France (F.R., L.S.-M., D.L.G.); INSERM U698, Cardiovascular Remodeling, Paris 7 University, CHU Xavier Bichat, Paris, France (M.D.H., L.L., J.B.M.); CEA-DRM-SHFJ, Orsay, France (F.H.); EA3964, Universite\u0301 Denis Diderot\u2013Paris 7, Paris, France (A.L., B.F); INSERM CIC 007; AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Centre d\u2019Investigation..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Hervatin","sequence":"additional","affiliation":[{"name":"From AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Service de Me\u0301decine Nucle\u0301aire; Universite\u0301 Denis Diderot\u2013Paris 7, UMR S773; and INSERM U773, Paris, France (F.R., L.S.-M., D.L.G.); INSERM U698, Cardiovascular Remodeling, Paris 7 University, CHU Xavier Bichat, Paris, France (M.D.H., L.L., J.B.M.); CEA-DRM-SHFJ, Orsay, France (F.H.); EA3964, Universite\u0301 Denis Diderot\u2013Paris 7, Paris, France (A.L., B.F); INSERM CIC 007; AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Centre d\u2019Investigation..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laure","family":"Sarda-Mantel","sequence":"additional","affiliation":[{"name":"From AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Service de Me\u0301decine Nucle\u0301aire; Universite\u0301 Denis Diderot\u2013Paris 7, UMR S773; and INSERM U773, Paris, France (F.R., L.S.-M., D.L.G.); INSERM U698, Cardiovascular Remodeling, Paris 7 University, CHU Xavier Bichat, Paris, France (M.D.H., L.L., J.B.M.); CEA-DRM-SHFJ, Orsay, France (F.H.); EA3964, Universite\u0301 Denis Diderot\u2013Paris 7, Paris, France (A.L., B.F); INSERM CIC 007; AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Centre d\u2019Investigation..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnes","family":"Lefort","sequence":"additional","affiliation":[{"name":"From AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Service de Me\u0301decine Nucle\u0301aire; Universite\u0301 Denis Diderot\u2013Paris 7, UMR S773; and INSERM U773, Paris, France (F.R., L.S.-M., D.L.G.); INSERM U698, Cardiovascular Remodeling, Paris 7 University, CHU Xavier Bichat, Paris, France (M.D.H., L.L., J.B.M.); CEA-DRM-SHFJ, Orsay, France (F.H.); EA3964, Universite\u0301 Denis Diderot\u2013Paris 7, Paris, France (A.L., B.F); INSERM CIC 007; AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Centre d\u2019Investigation..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Duval","sequence":"additional","affiliation":[{"name":"From AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Service de Me\u0301decine Nucle\u0301aire; Universite\u0301 Denis Diderot\u2013Paris 7, UMR S773; and INSERM U773, Paris, France (F.R., L.S.-M., D.L.G.); INSERM U698, Cardiovascular Remodeling, Paris 7 University, CHU Xavier Bichat, Paris, France (M.D.H., L.L., J.B.M.); CEA-DRM-SHFJ, Orsay, France (F.H.); EA3964, Universite\u0301 Denis Diderot\u2013Paris 7, Paris, France (A.L., B.F); INSERM CIC 007; AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Centre d\u2019Investigation..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liliane","family":"Louedec","sequence":"additional","affiliation":[{"name":"From AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Service de Me\u0301decine Nucle\u0301aire; Universite\u0301 Denis Diderot\u2013Paris 7, UMR S773; and INSERM U773, Paris, France (F.R., L.S.-M., D.L.G.); INSERM U698, Cardiovascular Remodeling, Paris 7 University, CHU Xavier Bichat, Paris, France (M.D.H., L.L., J.B.M.); CEA-DRM-SHFJ, Orsay, France (F.H.); EA3964, Universite\u0301 Denis Diderot\u2013Paris 7, Paris, France (A.L., B.F); 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Universite\u0301 Denis Diderot\u2013Paris 7, UMR S773; and INSERM U773, Paris, France (F.R., L.S.-M., D.L.G.); INSERM U698, Cardiovascular Remodeling, Paris 7 University, CHU Xavier Bichat, Paris, France (M.D.H., L.L., J.B.M.); CEA-DRM-SHFJ, Orsay, France (F.H.); EA3964, Universite\u0301 Denis Diderot\u2013Paris 7, Paris, France (A.L., B.F); INSERM CIC 007; AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Centre d\u2019Investigation..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Baptiste","family":"Michel","sequence":"additional","affiliation":[{"name":"From AP-HP, Groupe Hospitalier Bichat-Claude Bernard, Service de Me\u0301decine Nucle\u0301aire; Universite\u0301 Denis Diderot\u2013Paris 7, UMR S773; and INSERM U773, Paris, France (F.R., L.S.-M., D.L.G.); INSERM U698, Cardiovascular Remodeling, Paris 7 University, CHU Xavier Bichat, Paris, France (M.D.H., L.L., J.B.M.); CEA-DRM-SHFJ, Orsay, France (F.H.); EA3964, Universite\u0301 Denis Diderot\u2013Paris 7, Paris, France (A.L., B.F); 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