{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:02:19Z","timestamp":1791576139873,"version":"4.3.4"},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"15","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2008,10,7]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Background\u2014<\/jats:italic>\n            <\/jats:bold>\n            <jats:italic>Dicer<\/jats:italic>\n            , an RNAse III endonuclease critical for processing of pre-microRNAs (miRNAs) into mature 22-nucleotide miRNAs, has proven a useful target to dissect the significance of miRNAs biogenesis in mammalian biology.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            To circumvent the embryonic lethality associated with germline null mutations for\n            <jats:italic>Dicer<\/jats:italic>\n            , we triggered conditional\n            <jats:italic>Dicer<\/jats:italic>\n            loss through the use of a tamoxifen-inducible Cre recombinase in the postnatal murine myocardium. Targeted\n            <jats:italic>Dicer<\/jats:italic>\n            deletion in 3-week-old mice provoked premature death within 1 week accompanied by mild ventricular remodeling and dramatic atrial enlargement. In the adult myocardium, loss of\n            <jats:italic>Dicer<\/jats:italic>\n            induced rapid and dramatic biventricular enlargement, accompanied by myocyte hypertrophy, myofiber disarray, ventricular fibrosis, and strong induction of fetal gene transcripts. Comparative miRNA profiling revealed a set of miRNAs that imply causality between miRNA depletion and spontaneous cardiac remodeling.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            Overall, these results indicate that modifications in miRNA biogenesis affect both juvenile and adult myocardial morphology and function.\n          <\/jats:p>","DOI":"10.1161\/circulationaha.108.769984","type":"journal-article","created":{"date-parts":[[2008,9,23]],"date-time":"2008-09-23T00:44:05Z","timestamp":1222130645000},"page":"1567-1576","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":253,"title":["Conditional\n            <i>Dicer<\/i>\n            Gene Deletion in the Postnatal Myocardium Provokes Spontaneous Cardiac Remodeling"],"prefix":"10.1161","volume":"118","author":[{"given":"Paula A.","family":"da Costa Martins","sequence":"first","affiliation":[{"name":"From the Hubrecht Institute and Interuniversity Cardiology Institute Netherlands, Royal Netherlands Academy of Sciences, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., M.K., R.J.v.O., L.J.D.W.); Department of Medical Physiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., L.J.D.W.); Heart Failure Research Center, Academic Medical Center, Amsterdam, Netherlands (Y.M.P.); and Department of Pediatrics, Cincinnati Children\u2019s Hospital..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meriem","family":"Bourajjaj","sequence":"additional","affiliation":[{"name":"From the Hubrecht Institute and Interuniversity Cardiology Institute Netherlands, Royal Netherlands Academy of Sciences, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., M.K., R.J.v.O., L.J.D.W.); Department of Medical Physiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., L.J.D.W.); Heart Failure Research Center, Academic Medical Center, Amsterdam, Netherlands (Y.M.P.); and Department of Pediatrics, Cincinnati Children\u2019s Hospital..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Gladka","sequence":"additional","affiliation":[{"name":"From the Hubrecht Institute and Interuniversity Cardiology Institute Netherlands, Royal Netherlands Academy of Sciences, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., M.K., R.J.v.O., L.J.D.W.); Department of Medical Physiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., L.J.D.W.); Heart Failure Research Center, Academic Medical Center, Amsterdam, Netherlands (Y.M.P.); and Department of Pediatrics, Cincinnati Children\u2019s Hospital..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara","family":"Kortland","sequence":"additional","affiliation":[{"name":"From the Hubrecht Institute and Interuniversity Cardiology Institute Netherlands, Royal Netherlands Academy of Sciences, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., M.K., R.J.v.O., L.J.D.W.); Department of Medical Physiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., L.J.D.W.); Heart Failure Research Center, Academic Medical Center, Amsterdam, Netherlands (Y.M.P.); and Department of Pediatrics, Cincinnati Children\u2019s Hospital..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph J.","family":"van Oort","sequence":"additional","affiliation":[{"name":"From the Hubrecht Institute and Interuniversity Cardiology Institute Netherlands, Royal Netherlands Academy of Sciences, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., M.K., R.J.v.O., L.J.D.W.); Department of Medical Physiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., L.J.D.W.); Heart Failure Research Center, Academic Medical Center, Amsterdam, Netherlands (Y.M.P.); and Department of Pediatrics, Cincinnati Children\u2019s Hospital..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yigal M.","family":"Pinto","sequence":"additional","affiliation":[{"name":"From the Hubrecht Institute and Interuniversity Cardiology Institute Netherlands, Royal Netherlands Academy of Sciences, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., M.K., R.J.v.O., L.J.D.W.); Department of Medical Physiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., L.J.D.W.); Heart Failure Research Center, Academic Medical Center, Amsterdam, Netherlands (Y.M.P.); and Department of Pediatrics, Cincinnati Children\u2019s Hospital..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffery D.","family":"Molkentin","sequence":"additional","affiliation":[{"name":"From the Hubrecht Institute and Interuniversity Cardiology Institute Netherlands, Royal Netherlands Academy of Sciences, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., M.K., R.J.v.O., L.J.D.W.); Department of Medical Physiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., L.J.D.W.); Heart Failure Research Center, Academic Medical Center, Amsterdam, Netherlands (Y.M.P.); and Department of Pediatrics, Cincinnati Children\u2019s Hospital..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon J.","family":"De Windt","sequence":"additional","affiliation":[{"name":"From the Hubrecht Institute and Interuniversity Cardiology Institute Netherlands, Royal Netherlands Academy of Sciences, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., M.K., R.J.v.O., L.J.D.W.); Department of Medical Physiology, Division of Heart and Lungs, University Medical Center Utrecht, Utrecht, Netherlands (P.A.d.C.M., M.B., M.G., L.J.D.W.); Heart Failure Research Center, Academic Medical Center, Amsterdam, Netherlands (Y.M.P.); and Department of Pediatrics, Cincinnati Children\u2019s Hospital..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00428-8"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1121158"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2005.01.002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2004.12.002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1590"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.12.031"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.12.035"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.061170"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0608791103"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1139089"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000257913.42552.23"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1582"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2007.04.004"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.03.030"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710228105"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1725"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1569"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1253"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413394200"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504834102"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.89.1.20"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.18.13571"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000118597.54416.00"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.105.608968"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1356105"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200603008"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature03817"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1109020"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.11.3005"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/28867"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.107.687947"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1644"}],"container-title":["Circulation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCULATIONAHA.108.769984","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T06:23:00Z","timestamp":1715581380000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCULATIONAHA.108.769984"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10,7]]},"references-count":33,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2008,10,7]]}},"alternative-id":["10.1161\/CIRCULATIONAHA.108.769984"],"URL":"https:\/\/doi.org\/10.1161\/circulationaha.108.769984","relation":{},"ISSN":["0009-7322","1524-4539"],"issn-type":[{"value":"0009-7322","type":"print"},{"value":"1524-4539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10,7]]},"assertion":[{"value":"2008-09-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}