{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:47:26Z","timestamp":1790855246116,"version":"4.1.0"},"reference-count":42,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"19","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2008,11,4]]},"abstract":"<jats:p><jats:bold><jats:italic>Background\u2014<\/jats:italic><\/jats:bold>Reperfusion injury is insufficiently addressed in current clinical management of acute limb ischemia. Controlled reperfusion carries an enormous clinical potential and was tested in a new reality-driven rodent model.<\/jats:p><jats:p><jats:bold><jats:italic>Methods and Results\u2014<\/jats:italic><\/jats:bold>Acute hind-limb ischemia was induced in Wistar rats and maintained for 4 hours. Unlike previous tourniquets models, femoral vessels were surgically prepared to facilitate controlled reperfusion and to prevent venous stasis. Rats were randomized into an experimental group (n=7), in which limbs were selectively perfused with a cooled isotone heparin solution at a limited flow rate before blood flow was restored, and a conventional group (n=7; uncontrolled blood reperfusion). Rats were killed 4 hours after blood reperfusion. Nonischemic limbs served as controls. Ischemia\/reperfusion injury was significant in both groups; total wet-to-dry ratio was 159\u00b144% of normal (<jats:italic>P<\/jats:italic>=0.016), whereas muscle viability and contraction force were reduced to 65\u00b113% (<jats:italic>P<\/jats:italic>=0.016) and 45\u00b134% (<jats:italic>P<\/jats:italic>=0.045), respectively. Controlled reperfusion, however, attenuated reperfusion injury significantly. Tissue edema was less pronounced (132\u00b116% versus 185\u00b142%;<jats:italic>P<\/jats:italic>=0.011) and muscle viability (74\u00b111% versus 57\u00b19%;<jats:italic>P<\/jats:italic>=0.004) and contraction force (68\u00b140% versus 26\u00b17%;<jats:italic>P<\/jats:italic>=0.045) were better preserved than after uncontrolled reperfusion. Moreover, subsequent blood circulation as assessed by laser Doppler recovered completely after controlled reperfusion but stayed durably impaired after uncontrolled reperfusion (<jats:italic>P<\/jats:italic>=0.027).<\/jats:p><jats:p><jats:bold><jats:italic>Conclusions\u2014<\/jats:italic><\/jats:bold>Reperfusion injury was significantly alleviated by basic modifications of the initial reperfusion period in a new in vivo model of acute limb ischemia. With this model, systematic optimizations of according protocols may eventually translate into improved clinical management of acute limb ischemia.<\/jats:p>","DOI":"10.1161\/circulationaha.108.787754","type":"journal-article","created":{"date-parts":[[2008,10,21]],"date-time":"2008-10-21T01:09:42Z","timestamp":1224551382000},"page":"1920-1928","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":48,"title":["Basic Control of Reperfusion Effectively Protects Against Reperfusion Injury in a Realistic Rodent Model of Acute Limb Ischemia"],"prefix":"10.1161","volume":"118","author":[{"given":"Florian","family":"Dick","sequence":"first","affiliation":[{"name":"From the Department of Cardiovascular Surgery (F.D., J.L., M.-N.G., J.S., H.T.) and Division of Angiology (C.K.), Inselspital, and University of Berne, Berne, Switzerland; Imperial College Vascular Surgery Research Group, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Charing Cross Hospital, London, UK (F.D.); and Department of Hepatobiliary Pancreatic Surgery, Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital, Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhui","family":"Li","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Surgery (F.D., J.L., M.-N.G., J.S., H.T.) and Division of Angiology (C.K.), Inselspital, and University of Berne, Berne, Switzerland; Imperial College Vascular Surgery Research Group, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Charing Cross Hospital, London, UK (F.D.); and Department of Hepatobiliary Pancreatic Surgery, Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital, Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Noe\u0308lle","family":"Giraud","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Surgery (F.D., J.L., M.-N.G., J.S., H.T.) and Division of Angiology (C.K.), Inselspital, and University of Berne, Berne, Switzerland; Imperial College Vascular Surgery Research Group, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Charing Cross Hospital, London, UK (F.D.); and Department of Hepatobiliary Pancreatic Surgery, Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital, Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Kalka","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Surgery (F.D., J.L., M.-N.G., J.S., H.T.) and Division of Angiology (C.K.), Inselspital, and University of Berne, Berne, Switzerland; Imperial College Vascular Surgery Research Group, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Charing Cross Hospital, London, UK (F.D.); and Department of Hepatobiliary Pancreatic Surgery, Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital, Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juerg","family":"Schmidli","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Surgery (F.D., J.L., M.-N.G., J.S., H.T.) and Division of Angiology (C.K.), Inselspital, and University of Berne, Berne, Switzerland; Imperial College Vascular Surgery Research Group, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Charing Cross Hospital, London, UK (F.D.); and Department of Hepatobiliary Pancreatic Surgery, Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital, Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hendrik","family":"Tevaearai","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Surgery (F.D., J.L., M.-N.G., J.S., H.T.) and Division of Angiology (C.K.), Inselspital, and University of Berne, Berne, Switzerland; Imperial College Vascular Surgery Research Group, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Charing Cross Hospital, London, UK (F.D.); and Department of Hepatobiliary Pancreatic Surgery, Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital, Medical..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1053\/svas.2001.23158"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1078-5884(96)80257-7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2006.12.037"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1078-5884(96)80048-7"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199804163381603"},{"key":"e_1_3_2_6_2","first-page":"32","volume":"29","year":"1988","unstructured":"Jivegard L, Holm J, Schersten T. 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