{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:38:53Z","timestamp":1792679933642,"version":"4.5.0"},"reference-count":29,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"18","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2010,5,11]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Background\u2014<\/jats:italic>\n            <\/jats:bold>\n            C-kit is a receptor tyrosine kinase family member expressed in hematopoietic stem cells. C-kit is also transiently expressed in cardiomyocyte precursors during development and in a rare cell population in the normal adult heart. In the present study, the cardiomyogenic potential of c-kit\n            <jats:sup>+<\/jats:sup>\n            cells isolated from normal neonatal, normal adult, and infarcted adult mouse hearts was evaluated.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            Magnetic activated cell sorting was used to prepare c-kit\n            <jats:sup>+<\/jats:sup>\n            cells from the hearts of ACT-EGFP\/MHC-nLAC double transgenic mice. These animals exhibit widespread enhanced green fluorescent protein (EGFP) expression and cardiomyocyte-restricted nuclear \u03b2-galactosidase activity, thus permitting simultaneous tracking of cell survival and differentiation. A subset of the c-kit\n            <jats:sup>+<\/jats:sup>\n            cells from double transgenic neonatal hearts acquired a cardiomyogenic phenotype when cocultured with fetal cardiomyocytes (2.4% of all EGFP\n            <jats:sup>+<\/jats:sup>\n            cells screened) but rarely when cultured alone or when cocultured with mouse fibroblasts (0.03% and 0.05% of the EGFP\n            <jats:sup>+<\/jats:sup>\n            cells screened, respectively). In contrast, c-kit\n            <jats:sup>+<\/jats:sup>\n            cells from normal adult double transgenic hearts failed to undergo cardiomyogenic differentiation when cocultured with nontransgenic fetal cardiomyocytes (&gt;18 000 EGFP\n            <jats:sup>+<\/jats:sup>\n            cells screened) or when transplanted into normal or infarcted adult mouse hearts (14 EGFP\n            <jats:sup>+<\/jats:sup>\n            grafts examined). A single c-kit\n            <jats:sup>+<\/jats:sup>\n            cell from an infarcted double transgenic adult heart was observed to acquire a cardiomyogenic phenotype in coculture (&gt;37 000 EGFP\n            <jats:sup>+<\/jats:sup>\n            cells screened).\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            These data suggest that the ability of cardiac-resident c-kit\n            <jats:sup>+<\/jats:sup>\n            cells to acquire a cardiomyogenic phenotype is subject to temporal limitations or, alternatively, that the cardiomyogenic population is lost. Elucidation of the underlying molecular basis may permit robust cardiomyogenic induction in adult-derived cardiac c-kit\n            <jats:sup>+<\/jats:sup>\n            cells.\n          <\/jats:p>","DOI":"10.1161\/circulationaha.109.909093","type":"journal-article","created":{"date-parts":[[2010,4,27]],"date-time":"2010-04-27T02:01:50Z","timestamp":1272333710000},"page":"1992-2000","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":193,"title":["Cardiomyogenic Potential of C-Kit\n            <sup>+<\/sup>\n            \u2013Expressing Cells Derived From Neonatal and Adult Mouse Hearts"],"prefix":"10.1161","volume":"121","author":[{"given":"Marc-Michael","family":"Zaruba","sequence":"first","affiliation":[{"name":"From the Riley Heart Research Center, Herman B. Wells Center for Pediatric Research, and Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis (M.Z., M.S., S.R., L.J.F.); and Ludwig-Maximilians University, Klinikum Grosshadern, Medical Department I, Munich, Germany (M.Z.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Soonpaa","sequence":"additional","affiliation":[{"name":"From the Riley Heart Research Center, Herman B. Wells Center for Pediatric Research, and Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis (M.Z., M.S., S.R., L.J.F.); and Ludwig-Maximilians University, Klinikum Grosshadern, Medical Department I, Munich, Germany (M.Z.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean","family":"Reuter","sequence":"additional","affiliation":[{"name":"From the Riley Heart Research Center, Herman B. Wells Center for Pediatric Research, and Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis (M.Z., M.S., S.R., L.J.F.); and Ludwig-Maximilians University, Klinikum Grosshadern, Medical Department I, Munich, Germany (M.Z.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loren J.","family":"Field","sequence":"additional","affiliation":[{"name":"From the Riley Heart Research Center, Herman B. Wells Center for Pediatric Research, and Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis (M.Z., M.S., S.R., L.J.F.); and Ludwig-Maximilians University, Klinikum Grosshadern, Medical Department I, Munich, Germany (M.Z.)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","first-page":"29","volume":"68","year":"2006","unstructured":"Rubart M, Field LJ. Cardiac regeneration: repopulating the heart. Ann RevPhysiol. 2006; 68: 29\u201349.","journal-title":"Ann RevPhysiol"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2006.12.005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07427"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-004-4189-6"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.10.028"},{"key":"e_1_3_2_6_2","first-page":"894","volume":"118","year":"2008","unstructured":"Christoforou N, Miller RA, Hill CM, Jie CC, McCallion AS, Gearhart JD. Mouse ES cell-derived cardiac precursor cells are multipotent and facilitate identification of novel cardiac genes. J Clin Invest. 2008; 118: 894\u2013903.","journal-title":"J Clin Invest"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2006.10.002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808920106"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.107.161737"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27019"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2007.10.024"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00687-1"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706760104"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.107.761031"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000173297.53793.fa"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2003.09.028"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8140423"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.02.013"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02446"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-2-11"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature730"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature729"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2009.0041"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.108.178525"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1164680"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1997.272.1.H220"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0751"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000236754.21499.1c"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.107.150755"}],"container-title":["Circulation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCULATIONAHA.109.909093","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T05:39:40Z","timestamp":1715578780000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCULATIONAHA.109.909093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,5,11]]},"references-count":29,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2010,5,11]]}},"alternative-id":["10.1161\/CIRCULATIONAHA.109.909093"],"URL":"https:\/\/doi.org\/10.1161\/circulationaha.109.909093","relation":{},"ISSN":["0009-7322","1524-4539"],"issn-type":[{"value":"0009-7322","type":"print"},{"value":"1524-4539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,5,11]]},"assertion":[{"value":"2010-04-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}