{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T18:51:16Z","timestamp":1771267876853,"version":"3.50.1"},"reference-count":16,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11_suppl_1","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2010,9,14]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Background\u2014<\/jats:italic>\n            <\/jats:bold>\n            We investigated the effects of cardiopulmonary bypass (CPB) on the contractile response of human peripheral microvasculature to endothelin-1 (ET-1), examined the role of specific ET receptors and protein kinase C-alpha (PKC-\u03b1), and analyzed ET-1\u2013related gene\/protein expression in this response.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Methods and Results\u2014<\/jats:italic>\n            <\/jats:bold>\n            Human skeletal muscle arterioles (90 to 180 \u03bcm in diameter) were dissected from tissue harvested before and after CPB from 30 patients undergoing cardiac surgery. In vitro contractile response to ET-1 was assessed by videomicroscopy, with and without an endothelin-A (ET-A) receptor antagonist, an endothelin-B (ET-B) antagonist, or a PKC-\u03b1 inhibitor. The post-CPB contractile response of peripheral arterioles to ET-1 was significantly decreased compared with pre-CPB response. The response to ET-1 was significantly inhibited in the presence of the ET-A antagonist BQ123 but unchanged in the presence of the ET-B receptor antagonist BQ788. Pretreatment with the PKC-\u03b1 inhibitor safingol reversed ET-1\u2013induced response from contraction to relaxation. The total protein levels of ET-A and ET-B receptors were not altered after CPB. Microarray analysis showed no significant changes in the gene expression of ET receptors, ET-1\u2013related proteins, and protein kinases after CPB.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>Conclusions\u2014<\/jats:italic>\n            <\/jats:bold>\n            CPB decreases myogenic contractile function of human peripheral arterioles in response to ET-1. The contractile response to ET-1 is through activation of ET-A receptors and PKC-\u03b1. CPB has no effects on ET-1\u2013related gene\/protein expression. These results provide novel mechanisms of ET-1\u2013induced contraction in the setting of vasomotor dysfunction after cardiac surgery.\n          <\/jats:p>","DOI":"10.1161\/circulationaha.109.928226","type":"journal-article","created":{"date-parts":[[2010,9,13]],"date-time":"2010-09-13T21:03:47Z","timestamp":1284411827000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":20,"title":["Effects of Cardiopulmonary Bypass on Endothelin-1\u2013Induced Contraction and Signaling in Human Skeletal Muscle Microcirculation"],"prefix":"10.1161","volume":"122","author":[{"given":"Jun","family":"Feng","sequence":"first","affiliation":[{"name":"From the Division of Cardiothoracic Surgery (J.F., L.M.C., M.P.R., R.T.C., Y.L., F.W.S.), Department of Surgery, Cardiovascular Research Center, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI; and the Division of Cardiothoracic Surgery (K.R.K., R.H., R.M.O.), Department of Surgery, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louis M.","family":"Chu","sequence":"additional","affiliation":[{"name":"From the Division of Cardiothoracic Surgery (J.F., L.M.C., M.P.R., R.T.C., Y.L., F.W.S.), Department of Surgery, Cardiovascular Research Center, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI; and the Division of Cardiothoracic Surgery (K.R.K., R.H., R.M.O.), Department of Surgery, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael P.","family":"Robich","sequence":"additional","affiliation":[{"name":"From the Division of Cardiothoracic Surgery (J.F., L.M.C., M.P.R., R.T.C., Y.L., F.W.S.), Department of Surgery, Cardiovascular Research Center, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI; and the Division of Cardiothoracic Surgery (K.R.K., R.H., R.M.O.), Department of Surgery, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard T.","family":"Clements","sequence":"additional","affiliation":[{"name":"From the Division of Cardiothoracic Surgery (J.F., L.M.C., M.P.R., R.T.C., Y.L., F.W.S.), Department of Surgery, Cardiovascular Research Center, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI; and the Division of Cardiothoracic Surgery (K.R.K., R.H., R.M.O.), Department of Surgery, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamal R.","family":"Khabbaz","sequence":"additional","affiliation":[{"name":"From the Division of Cardiothoracic Surgery (J.F., L.M.C., M.P.R., R.T.C., Y.L., F.W.S.), Department of Surgery, Cardiovascular Research Center, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI; and the Division of Cardiothoracic Surgery (K.R.K., R.H., R.M.O.), Department of Surgery, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Hagberg","sequence":"additional","affiliation":[{"name":"From the Division of Cardiothoracic Surgery (J.F., L.M.C., M.P.R., R.T.C., Y.L., F.W.S.), Department of Surgery, Cardiovascular Research Center, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI; and the Division of Cardiothoracic Surgery (K.R.K., R.H., R.M.O.), Department of Surgery, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhong","family":"Liu","sequence":"additional","affiliation":[{"name":"From the Division of Cardiothoracic Surgery (J.F., L.M.C., M.P.R., R.T.C., Y.L., F.W.S.), Department of Surgery, Cardiovascular Research Center, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI; and the Division of Cardiothoracic Surgery (K.R.K., R.H., R.M.O.), Department of Surgery, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M.","family":"Osipov","sequence":"additional","affiliation":[{"name":"From the Division of Cardiothoracic Surgery (J.F., L.M.C., M.P.R., R.T.C., Y.L., F.W.S.), Department of Surgery, Cardiovascular Research Center, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI; and the Division of Cardiothoracic Surgery (K.R.K., R.H., R.M.O.), Department of Surgery, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank W.","family":"Sellke","sequence":"additional","affiliation":[{"name":"From the Division of Cardiothoracic Surgery (J.F., L.M.C., M.P.R., R.T.C., Y.L., F.W.S.), Department of Surgery, Cardiovascular Research Center, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI; and the Division of Cardiothoracic Surgery (K.R.K., R.H., R.M.O.), Department of Surgery, Beth Israel Deaconess Medical Center, and Harvard Medical School, Boston, Mass."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcts.2004.07.040"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1067\/msy.2003.229"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000080325.94345.8B"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1213\/00000539-199608000-00015"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2007.03.010"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.61.1.391"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200201000-00014"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1053\/jcan.2000.9451"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1067\/mtc.2000.109700"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1010-7940(05)80161-7"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1067\/mtc.2001.114936"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011452108131"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2008.06.076"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1067\/mtc.2002.121972"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.107.755827"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(03)00969-3"}],"container-title":["Circulation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCULATIONAHA.109.928226","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T06:02:39Z","timestamp":1715580159000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCULATIONAHA.109.928226"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,14]]},"references-count":16,"journal-issue":{"issue":"11_suppl_1","published-print":{"date-parts":[[2010,9,14]]}},"alternative-id":["10.1161\/CIRCULATIONAHA.109.928226"],"URL":"https:\/\/doi.org\/10.1161\/circulationaha.109.928226","relation":{},"ISSN":["0009-7322","1524-4539"],"issn-type":[{"value":"0009-7322","type":"print"},{"value":"1524-4539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,14]]},"assertion":[{"value":"2010-09-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}