{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:06:37Z","timestamp":1791176797659,"version":"4.1.0"},"reference-count":26,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"20","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2010,11,16]]},"abstract":"<jats:sec>\n            <jats:title>Background\u2014<\/jats:title>\n            <jats:p>It has been proposed that bone marrow\u2013derived cells infiltrate the neointima, where they differentiate into smooth muscle (SM) cells; however, technical limitations have hindered clear identification of the lineages of bone marrow\u2013derived \u201cSM cell\u2013like\u201d cells.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods and Results\u2014<\/jats:title>\n            <jats:p>\n              Using a specific antibody against the definitive SM cell lineage marker SM myosin heavy chain (SM-MHC) and mouse lines in which reporter genes were driven by regulatory programs for either\n              <jats:italic>SM-MHC<\/jats:italic>\n              or\n              <jats:italic>SM<\/jats:italic>\n              \u03b1\n              <jats:italic>-actin<\/jats:italic>\n              , we demonstrated that although some bone marrow\u2013derived cells express SM \u03b1-actin in the wire injury\u2013induced neointima, those cells did not express SM-MHC, even 30 weeks after injury. Likewise, no SM-MHC\n              <jats:sup>+<\/jats:sup>\n              bone marrow\u2013derived cells were found in vascular lesions in\n              <jats:italic>apolipoprotein E<\/jats:italic>\n              <jats:sup>\u2212\/\u2212<\/jats:sup>\n              mice or in a heart transplantation vasculopathy model. Instead, the majority of bone marrow\u2013derived SM \u03b1-actin\n              <jats:sup>+<\/jats:sup>\n              cells were also CD115\n              <jats:sup>+<\/jats:sup>\n              CD11b\n              <jats:sup>+<\/jats:sup>\n              F4\/80\n              <jats:sup>+<\/jats:sup>\n              Ly-6C\n              <jats:sup>+<\/jats:sup>\n              , which is the surface phenotype of inflammatory monocytes. Moreover, adoptively transferred CD11b\n              <jats:sup>+<\/jats:sup>\n              Ly-6C\n              <jats:sup>+<\/jats:sup>\n              bone marrow cells expressed SM \u03b1-actin in the injured artery. Expression of inflammation-related genes was significantly higher in neointimal subregions rich in bone marrow\u2013derived SM \u03b1-actin\n              <jats:sup>+<\/jats:sup>\n              cells than in other regions.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions\u2014<\/jats:title>\n            <jats:p>\n              It appears that bone marrow\u2013derived SM \u03b1-actin\n              <jats:sup>+<\/jats:sup>\n              cells are of monocyte\/macrophage lineage and are involved in vascular remodeling. It is very unlikely that these cells acquire the definitive SM cell lineage.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circulationaha.110.965202","type":"journal-article","created":{"date-parts":[[2010,11,2]],"date-time":"2010-11-02T05:21:43Z","timestamp":1288675303000},"page":"2048-2057","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":108,"title":["Bone Marrow\u2013Derived Cells Contribute to Vascular Inflammation but Do Not Differentiate Into Smooth Muscle Cell Lineages"],"prefix":"10.1161","volume":"122","author":[{"given":"Hiroshi","family":"Iwata","sequence":"first","affiliation":[{"name":"From the Department of Cardiovascular Medicine (H.I., I.M., K.F., N.T., K.E., R.N.), Global COE Program (I.M., R.N.), Comprehensive Center of Education and Research for Chemical Biology of the Diseases (K.F., R.N.), and Department of Cardiovascular Surgery (T.Y.), Graduate School of Medicine, University of Tokyo, Tokyo, Japan; PRESTO, Japan Science and Technology Agency, Saitama, Japan (I.M.); Kyowa Hakko Kirin Co, Ltd, Tokyo, Japan (A.F.); Department of Pathology, University of Texas Southwestern..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ichiro","family":"Manabe","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Medicine (H.I., I.M., K.F., N.T., K.E., R.N.), Global COE Program (I.M., R.N.), Comprehensive Center of Education and Research for Chemical Biology of the Diseases (K.F., R.N.), and Department of Cardiovascular Surgery (T.Y.), Graduate School of Medicine, University of Tokyo, Tokyo, Japan; PRESTO, Japan Science and Technology Agency, Saitama, Japan (I.M.); Kyowa Hakko Kirin Co, Ltd, Tokyo, Japan (A.F.); Department of Pathology, University of Texas Southwestern..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhito","family":"Fujiu","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Medicine (H.I., I.M., K.F., N.T., K.E., R.N.), Global COE Program (I.M., R.N.), Comprehensive Center of Education and Research for Chemical Biology of the Diseases (K.F., R.N.), and Department of Cardiovascular Surgery (T.Y.), Graduate School of Medicine, University of Tokyo, Tokyo, Japan; PRESTO, Japan Science and Technology Agency, Saitama, Japan (I.M.); Kyowa Hakko Kirin Co, Ltd, Tokyo, Japan (A.F.); Department of Pathology, University of Texas Southwestern..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsufumi","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Medicine (H.I., I.M., K.F., N.T., K.E., R.N.), Global COE Program (I.M., R.N.), Comprehensive Center of Education and Research for Chemical Biology of the Diseases (K.F., R.N.), and Department of Cardiovascular Surgery (T.Y.), Graduate School of Medicine, University of Tokyo, Tokyo, Japan; PRESTO, Japan Science and Technology Agency, Saitama, Japan (I.M.); Kyowa Hakko Kirin Co, Ltd, Tokyo, Japan (A.F.); Department of Pathology, University of Texas Southwestern..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norifumi","family":"Takeda","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Medicine (H.I., I.M., K.F., N.T., K.E., R.N.), Global COE Program (I.M., R.N.), Comprehensive Center of Education and Research for Chemical Biology of the Diseases (K.F., R.N.), and Department of Cardiovascular Surgery (T.Y.), Graduate School of Medicine, University of Tokyo, Tokyo, Japan; PRESTO, Japan Science and Technology Agency, Saitama, Japan (I.M.); Kyowa Hakko Kirin Co, Ltd, Tokyo, Japan (A.F.); Department of Pathology, University of Texas Southwestern..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosei","family":"Eguchi","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Medicine (H.I., I.M., K.F., N.T., K.E., R.N.), Global COE Program (I.M., R.N.), Comprehensive Center of Education and Research for Chemical Biology of the Diseases (K.F., R.N.), and Department of Cardiovascular Surgery (T.Y.), Graduate School of Medicine, University of Tokyo, Tokyo, Japan; PRESTO, Japan Science and Technology Agency, Saitama, Japan (I.M.); Kyowa Hakko Kirin Co, Ltd, Tokyo, Japan (A.F.); Department of Pathology, University of Texas Southwestern..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Furuya","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Medicine (H.I., I.M., K.F., N.T., K.E., R.N.), Global COE Program (I.M., R.N.), Comprehensive Center of Education and Research for Chemical Biology of the Diseases (K.F., R.N.), and Department of Cardiovascular Surgery (T.Y.), Graduate School of Medicine, University of Tokyo, Tokyo, Japan; PRESTO, Japan Science and Technology Agency, Saitama, Japan (I.M.); Kyowa Hakko Kirin Co, Ltd, Tokyo, Japan (A.F.); Department of Pathology, University of Texas Southwestern..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Kuro-o","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Medicine (H.I., I.M., K.F., N.T., K.E., R.N.), Global COE Program (I.M., R.N.), Comprehensive Center of Education and Research for Chemical Biology of the Diseases (K.F., R.N.), and Department of Cardiovascular Surgery (T.Y.), Graduate School of Medicine, University of Tokyo, Tokyo, Japan; PRESTO, Japan Science and Technology Agency, Saitama, Japan (I.M.); Kyowa Hakko Kirin Co, Ltd, Tokyo, Japan (A.F.); Department of Pathology, University of Texas Southwestern..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masataka","family":"Sata","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Medicine (H.I., I.M., K.F., N.T., K.E., R.N.), Global COE Program (I.M., R.N.), Comprehensive Center of Education and Research for Chemical Biology of the Diseases (K.F., R.N.), and Department of Cardiovascular Surgery (T.Y.), Graduate School of Medicine, University of Tokyo, Tokyo, Japan; PRESTO, Japan Science and Technology Agency, Saitama, Japan (I.M.); Kyowa Hakko Kirin Co, Ltd, Tokyo, Japan (A.F.); Department of Pathology, University of Texas Southwestern..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryozo","family":"Nagai","sequence":"additional","affiliation":[{"name":"From the Department of Cardiovascular Medicine (H.I., I.M., K.F., N.T., K.E., R.N.), Global COE Program (I.M., R.N.), Comprehensive Center of Education and Research for Chemical Biology of the Diseases (K.F., R.N.), and Department of Cardiovascular Surgery (T.Y.), Graduate School of Medicine, University of Tokyo, Tokyo, Japan; PRESTO, Japan Science and Technology Agency, Saitama, Japan (I.M.); Kyowa Hakko Kirin Co, Ltd, Tokyo, Japan (A.F.); Department of Pathology, University of Texas Southwestern..."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00041.2003"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11883-003-0027-9"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(99)00122-8"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0402-403"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000031525.61826.a8"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.atv.0000249623.79871.bc"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.87.5.363"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.4.1047"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90140-7"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/35014065"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000096651.13001.b4"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000190613.22565.13"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411585200"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2008.09.018"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00875.2001"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)00313-X"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1178331"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2006.05.050"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11883-000-0081-5"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.021908.132557"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11385"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000266448.30370.a0"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2010.01.027"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.0000126417.38728.f6"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.atv.0000188554.49745.9e"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700654"}],"container-title":["Circulation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/CIRCULATIONAHA.110.965202","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T05:35:30Z","timestamp":1715578530000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/CIRCULATIONAHA.110.965202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,11,16]]},"references-count":26,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2010,11,16]]}},"alternative-id":["10.1161\/CIRCULATIONAHA.110.965202"],"URL":"https:\/\/doi.org\/10.1161\/circulationaha.110.965202","relation":{},"ISSN":["0009-7322","1524-4539"],"issn-type":[{"value":"0009-7322","type":"print"},{"value":"1524-4539","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,11,16]]},"assertion":[{"value":"2010-05-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-08-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}