{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:42:57Z","timestamp":1791981777899,"version":"4.4.0"},"reference-count":50,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"9","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2019,2,26]]},"abstract":"<jats:sec>\n            <jats:title>Background:<\/jats:title>\n            <jats:p>Inflammatory response after myocardial infarction (MI) is essential for cardiac healing, whereas excessive and prolonged inflammation extends the infarction and promotes adverse cardiac remodeling. Understanding the mechanistic insight of these tightly controlled inflammatory processes has a significant impact on post-MI recovery and therapy. Here, we uncover the critical role of small GTPase RhoE in post-MI recovery and its clinical implication.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>Three genetic mouse lines are used: global RhoE knockout, cardiomyocyte-specific RhoE heterozygous, and cardiomyocyte-specific RhoE overexpression mice. A set of molecular signaling experiments, including bimolecular fluorescence complementation, immunoprecipitation, electrophoretic mobility shift assay, and mRNA microarray analysis, were conducted. Permanent ligation of the left anterior descending artery was performed, followed by the assessments of cardiac function, inflammation, and survival in the first week after MI. Finally, we examined the correlation of the expression levels of RhoE in MI patient heart and patient prognosis.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>RhoE deficiency turns on a group of proinflammatory gene expressions in mouse heart. Mice with cardiomyocyte-specific haploinsufficiency exhibit excessive inflammatory response with deleterious cardiac function after MI. A profound increase in nuclear factor-\u03baB activity is detected in the mutant heart and the isolated cardiomyocytes. We further find that the expression of RhoE is upregulated in response to MI. Mechanistically, RhoE interacts with p65 and p50 individually in cytosol and blocks their nuclear translocation. RhoE also occupies the dimerization domain of p65 and subsequently disrupts the heterodimerization between p65 and p50. Cardiac RhoE overexpression inhibits nuclear factor-\u03baB activity, restrains post-MI inflammation, and improves cardiac function and survival. Consistently, we find that the expression level of RhoE is elevated in the heart of patients with MI and that the patients with a higher expression level of RhoE exhibit a better prognosis in cardiac function recovery.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>The study uncovers RhoE as a new fine-tuning factor modulating MI-induced inflammation and promoting injured heart recovery. RhoE may serve as a new potential biomarker for the assessment of MI patient prognosis. Manipulation of RhoE could be as a potential therapeutic approach for MI and other inflammatory diseases.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/circulationaha.118.033700","type":"journal-article","created":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T13:44:06Z","timestamp":1547732646000},"page":"1185-1198","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":55,"title":["RhoE Fine-Tunes Inflammatory Response in Myocardial Infarction"],"prefix":"10.1161","volume":"139","author":[{"given":"Yuan","family":"Dai","sequence":"first","affiliation":[{"name":"Center for Translational Cancer Research, Texas A&amp;M University Institute of Biosciences and Technology, Houston (Y.D., W.L., T.Y., X.L., W.L., K.C.A., J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangping","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China (J.S., X.W., S.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjiao","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Texas A&amp;M University Institute of Biosciences and Technology, Houston (Y.D., W.L., T.Y., X.L., W.L., K.C.A., J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingli","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Texas A&amp;M University Institute of Biosciences and Technology, Houston (Y.D., W.L., T.Y., X.L., W.L., K.C.A., J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, China (X. Yue)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Lin","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Texas A&amp;M University Institute of Biosciences and Technology, Houston (Y.D., W.L., T.Y., X.L., W.L., K.C.A., J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangsheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangzhou Biotron Technology Co Ltd, Guangzhou, China (X. Yang)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijia","family":"Luo","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Texas A&amp;M University Institute of Biosciences and Technology, Houston (Y.D., W.L., T.Y., X.L., W.L., K.C.A., J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junli","family":"Guo","sequence":"additional","affiliation":[{"name":"Cardiovascular Disease and Research Institute, First Affiliated Hospital, Key Laboratory of Tropical Diseases and Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China (J.G.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China (J.S., X.W., S.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songqing","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China (J.S., X.W., S.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelsey C.","family":"Andrade","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Texas A&amp;M University Institute of Biosciences and Technology, Houston (Y.D., W.L., T.Y., X.L., W.L., K.C.A., J.C.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiang","family":"Chang","sequence":"additional","affiliation":[{"name":"Center for Translational Cancer Research, Texas A&amp;M University Institute of Biosciences and Technology, Houston (Y.D., W.L., T.Y., X.L., 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