{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:56:35Z","timestamp":1790193395954,"version":"4.1.0"},"reference-count":40,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"14","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2025,4,8]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND:<\/jats:title>\n                    <jats:p>Cytokinesis is the last step in the eukaryotic cell cycle, which physically separates a mitotic cell into 2 daughter cells. A few days after birth in mouse cardiomyocytes, DNA synthesis occurs without cytokinesis, leading to the majority of cardiomyocytes becoming binucleated instead of generating 2 daughter cells with 1 nucleus each. This results in cell cycle arrest of cardiomyocytes, and the mouse heart is no longer able to regenerate. A longstanding unanswered question is whether binucleation of cardiomyocytes is a result of cytokinesis failure.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>To address this, we generated several transgenic mouse models to determine whether forced induction of cardiomyocyte cytokinesis generates mononucleated cardiomyocytes and restores the endogenous regenerative properties of the myocardium. We focused on 2 complementary regulators of cytokinesis: Plk1 (polo-like kinase 1) and Ect2 (epithelial cell-transformation sequence 2).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>We found that cardiomyocyte-specific transgenic overexpression of constitutively active Plk1(T210D) promotes mitosis and cytokinesis in adult hearts, whereas overexpression of Ect2 alone promotes only cytokinesis. Cardiomyocyte-specific overexpression of both Plk1(T210D) and Ect2 concomitantly (double transgenic) prevents binucleation of cardiomyocytes postnatally and results in widespread cardiomyocyte mitosis, cardiac enlargement, contractile failure, and death before 2 weeks of age. In contrast, doxycycline-inducible cardiomyocyte-specific overexpression of both genes (inducible double transgenic) in the adult heart results in cardiomyocyte mitosis and transient contractile dysfunction. Importantly, this transient induction of cytokinesis in adult mice improves left ventricular systolic function after myocardial infarction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS:<\/jats:title>\n                    <jats:p>These results collectively demonstrate that cytokinesis failure mediates cardiomyocyte multinucleation and cell cycle exit of postnatal cardiomyocytes, but may be a protective mechanism to preserve the contractile function of the myocardium.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/circulationaha.124.065763","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T05:00:23Z","timestamp":1738818023000},"page":"1009-1023","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":14,"title":["Induced Cytokinesis Generates Highly Proliferative Mononuclear Cardiomyocytes at the Expense of Contractility"],"prefix":"10.1161","volume":"151","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9446-7378","authenticated-orcid":true,"given":"Nicholas T.","family":"Lam","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, Dallas."},{"name":"Sarver Heart Center and Department of Medicine, Division of Cardiology, The University of Arizona, Tucson (N.T.L., W.M.E., I.M.-M., H.A.S.)."},{"name":"Heart Research Institute, Sydney, Australia (N.T.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0694-3176","authenticated-orcid":true,"given":"Ngoc Uyen Nhi","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6942-3680","authenticated-orcid":true,"given":"Waleed M.","family":"Elhelaly","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, Dallas."},{"name":"Sarver Heart Center and Department of Medicine, Division of Cardiology, The University of Arizona, Tucson (N.T.L., W.M.E., I.M.-M., H.A.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Cheng","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Menendez-Montes","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, Dallas."},{"name":"Sarver Heart Center and Department of Medicine, Division of Cardiology, The University of Arizona, Tucson (N.T.L., W.M.E., I.M.-M., H.A.S.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1241-6826","authenticated-orcid":true,"given":"Shah R.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelson","family":"Vo","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Briard","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lobna","family":"El-Feky","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qamar M.","family":"Omari","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., 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Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5600-4698","authenticated-orcid":true,"given":"Mahmoud Salama","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, Dallas."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Cardiology (N.T.L., N.U.N.N., W.M.E., C.-C.H., I.M.-M., F.X., S.R.A., N.V., N.B., L.E.-F., Q.M.O., A.C.C., M.S.A., S.L., S.T., H.A.S.), The University of Texas Southwestern Medical Center, 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