{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:53:23Z","timestamp":1761540803062},"reference-count":33,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"21","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation"],"published-print":{"date-parts":[[2001,11,20]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>\n                <jats:bold>\n                  <jats:italic>Background<\/jats:italic>\n                <\/jats:bold>\n              <\/jats:italic>\n            <\/jats:bold>\n            Extracellular matrix (ECM) remodeling is central to the development of restenosis after coronary angioplasty (PTCA). As a regulator of ECM deposition by vascular cells, substantial evidence implicates transforming growth factor-\u03b21 (TGF-\u03b21) in the pathogenesis of restenosis. We investigated the effects of intracoronary expression of a transgenic antagonist of TGF-\u03b21 on luminal loss after PTCA.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>\n                <jats:bold>\n                  <jats:italic>Methods and Results<\/jats:italic>\n                <\/jats:bold>\n              <\/jats:italic>\n            <\/jats:bold>\n            Porcine coronary arteries were randomized to receive a recombinant adenovirus expressing a secreted form of TGF-\u03b2 type II receptor (Ad5-RIIs), an adenovirus expressing \u03b2-galactosidase (Ad5-\n            <jats:italic>lacZ<\/jats:italic>\n            ), or vehicle only by intramural injection at the site of PTCA. Computerized morphometry 28 days after angioplasty revealed a greater minimum luminal area in Ad5-RIIs\u2013injected arteries (1.71\u00b10.12 mm\n            <jats:sup>2<\/jats:sup>\n            ) than in the Ad5-\n            <jats:italic>lacZ<\/jats:italic>\n            (1.33\u00b10.13 mm\n            <jats:sup>2<\/jats:sup>\n            ) or vehicle-only (1.08\u00b10.17 mm\n            <jats:sup>2<\/jats:sup>\n            ;\n            <jats:italic>P<\/jats:italic>\n            =0.010 by ANOVA) groups. This was accompanied by greater areas within the internal (\n            <jats:italic>P<\/jats:italic>\n            =0.013) and external (\n            <jats:italic>P<\/jats:italic>\n            =0.031) elastic laminae in Ad5-RIIs\u2013treated vessels. Adventitial collagen content at the site of injury was increased in the Ad5-RIIs group, in contrast to decreases in the Ad5-\n            <jats:italic>lacZ<\/jats:italic>\n            and vehicle-only groups (\n            <jats:italic>P<\/jats:italic>\n            =0.004).\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>\n                <jats:bold>\n                  <jats:italic>Conclusions<\/jats:italic>\n                <\/jats:bold>\n              <\/jats:italic>\n            <\/jats:bold>\n            Adenovirus-mediated antagonism of TGF-\u03b21 at the site of PTCA reduces luminal loss after PTCA by inhibiting constrictive remodeling. Antagonism of TGF-\u03b21 stimulates the formation of a dense collagenous adventitia, which prevents constrictive remodeling by acting as an external scaffold. These findings demonstrate the potential of gene therapy\u2013mediated antagonism of TGF-\u03b21 as prophylactic therapy for restenosis.\n          <\/jats:p>","DOI":"10.1161\/hc4601.099405","type":"journal-article","created":{"date-parts":[[2007,9,28]],"date-time":"2007-09-28T18:31:09Z","timestamp":1191004269000},"page":"2595-2601","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":52,"title":["Adenovirus-Mediated Gene Transfer of a Secreted Transforming Growth Factor-\u03b2 Type II Receptor Inhibits Luminal Loss and Constrictive Remodeling After Coronary Angioplasty and Enhances Adventitial Collagen Deposition"],"prefix":"10.1161","volume":"104","author":[{"given":"Paul A.","family":"Kingston","sequence":"first","affiliation":[{"name":"From the Molecular Medicine and Gene Therapy Unit, University of Manchester (P.A.K., A.D., M.G.C., P.R.L.), and Department of Medicine, Manchester Royal Infirmary (S.S., A.M.H.), Manchester, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjay","family":"Sinha","sequence":"additional","affiliation":[{"name":"From the Molecular Medicine and Gene Therapy Unit, University of Manchester (P.A.K., A.D., M.G.C., P.R.L.), and Department of Medicine, Manchester Royal Infirmary (S.S., A.M.H.), Manchester, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"David","sequence":"additional","affiliation":[{"name":"From the Molecular Medicine and Gene Therapy Unit, University of Manchester (P.A.K., A.D., M.G.C., P.R.L.), and Department of Medicine, Manchester Royal Infirmary (S.S., A.M.H.), Manchester, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria G.","family":"Castro","sequence":"additional","affiliation":[{"name":"From the Molecular Medicine and Gene Therapy Unit, University of Manchester (P.A.K., A.D., M.G.C., P.R.L.), and Department of Medicine, Manchester Royal Infirmary (S.S., A.M.H.), Manchester, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro R.","family":"Lowenstein","sequence":"additional","affiliation":[{"name":"From the Molecular Medicine and Gene Therapy Unit, University of Manchester (P.A.K., A.D., M.G.C., P.R.L.), and Department of Medicine, Manchester Royal Infirmary (S.S., A.M.H.), Manchester, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony M.","family":"Heagerty","sequence":"additional","affiliation":[{"name":"From the Molecular Medicine and Gene Therapy Unit, University of Manchester (P.A.K., A.D., M.G.C., P.R.L.), and Department of Medicine, Manchester Royal Infirmary (S.S., A.M.H.), Manchester, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.98.3.200"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(92)90389-5"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(98)00203-X"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240450202"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)81802-6"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(88)90224-0"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.15.5287"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0033-0620(98)80021-2"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116027"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/1054-8807(94)90008-6"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvb.15.11.1951"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/atvb.16.10.1298"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.1995.0054"},{"key":"e_1_3_3_14_2","doi-asserted-by":"crossref","unstructured":"Southgate TD Kingston PA Castro MG. 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