{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T17:35:06Z","timestamp":1790012106543,"version":"4.0.1"},"reference-count":40,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2002,2,22]]},"abstract":"<jats:p>\n            Familial hypertrophic cardiomyopathy (HCM) is caused by mutations in 9 sarcomeric protein genes. The most commonly affected is \u03b2-myosin heavy chain (\n            <jats:italic>MYH7<\/jats:italic>\n            ), where missense mutations cluster in the head and neck regions and directly affect motor function. Comparable mutations have not been described in the light meromyosin (LMM) region of the myosin rod, nor would these be expected to directly affect motor function. We studied 82 probands with HCM in whom no mutations had been found in\n            <jats:italic>MYH7<\/jats:italic>\n            exons encoding the head and neck regions of myosin nor in the other frequently implicated disease genes. Primers were designed to amplify exons 24 to 40 of\n            <jats:italic>MYH7<\/jats:italic>\n            . These amplimers were subjected to temperature modulated heteroduplex analysis by denaturing high-performance liquid chromatography. An Ala1379Thr missense mutation in exon 30 segregated with disease in three families and was not present in 200 normal chromosomes. The mutation occurred on two haplotypes, indicating that it was not a polymorphism linked with another disease-causing mutation. The position of this residue within the LMM region of myosin suggests that it may be important for thick filament assembly or for accessory protein binding. A further missense mutation in exon 37, Ser1776Gly, segregated with disease in a single family and was absent from 400 population-matched control chromosomes. Because the Ser1776 residue occupies a core position in the myosin rod at which the substitution of glycine is extremely energetically unfavorable, it is likely to disrupt the coiled-coil structure. We conclude that mutation of the LMM can cause HCM and that such mutations may act through novel mechanisms of disease pathogenesis involving myosin filament assembly or interaction with thick filament binding proteins.\n          <\/jats:p>","DOI":"10.1161\/hh0302.104532","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T23:16:47Z","timestamp":1027898207000},"page":"263-269","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":79,"title":["Mutations of the Light Meromyosin Domain of the \u03b2-Myosin Heavy Chain Rod in Hypertrophic Cardiomyopathy"],"prefix":"10.1161","volume":"90","author":[{"given":"Edward","family":"Blair","sequence":"first","affiliation":[{"name":"From the Departments of Cardiovascular Medicine (E.B., C.R., M.J.O., H.W.) and Paediatric Cardiology (I.O.S.), University of Oxford and John Radcliffe Hospital, Oxford, UK; the US\/MRC Centre for Molecular and Cellular Biology (J.C.M.S., V.A.C.) and the Department of Internal Medicine and Tygerberg Hospital (P.B.), University of Stellenbosch, Tygerberg, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Redwood","sequence":"additional","affiliation":[{"name":"From the Departments of Cardiovascular Medicine (E.B., C.R., M.J.O., H.W.) and Paediatric Cardiology (I.O.S.), University of Oxford and John Radcliffe Hospital, Oxford, UK; the US\/MRC Centre for Molecular and Cellular Biology (J.C.M.S., V.A.C.) and the Department of Internal Medicine and Tygerberg Hospital (P.B.), University of Stellenbosch, Tygerberg, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa","family":"de Jesus Oliveira","sequence":"additional","affiliation":[{"name":"From the Departments of Cardiovascular Medicine (E.B., C.R., M.J.O., H.W.) and Paediatric Cardiology (I.O.S.), University of Oxford and John Radcliffe Hospital, Oxford, UK; the US\/MRC Centre for Molecular and Cellular Biology (J.C.M.S., V.A.C.) and the Department of Internal Medicine and Tygerberg Hospital (P.B.), University of Stellenbosch, Tygerberg, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.C.","family":"Moolman-Smook","sequence":"additional","affiliation":[{"name":"From the Departments of Cardiovascular Medicine (E.B., C.R., M.J.O., H.W.) and Paediatric Cardiology (I.O.S.), University of Oxford and John Radcliffe Hospital, Oxford, UK; the US\/MRC Centre for Molecular and Cellular Biology (J.C.M.S., V.A.C.) and the Department of Internal Medicine and Tygerberg Hospital (P.B.), University of Stellenbosch, Tygerberg, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Brink","sequence":"additional","affiliation":[{"name":"From the Departments of Cardiovascular Medicine (E.B., C.R., M.J.O., H.W.) and Paediatric Cardiology (I.O.S.), University of Oxford and John Radcliffe Hospital, Oxford, UK; the US\/MRC Centre for Molecular and Cellular Biology (J.C.M.S., V.A.C.) and the Department of Internal Medicine and Tygerberg Hospital (P.B.), University of Stellenbosch, Tygerberg, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.A.","family":"Corfield","sequence":"additional","affiliation":[{"name":"From the Departments of Cardiovascular Medicine (E.B., C.R., M.J.O., H.W.) and Paediatric Cardiology (I.O.S.), University of Oxford and John Radcliffe Hospital, Oxford, UK; the US\/MRC Centre for Molecular and Cellular Biology (J.C.M.S., V.A.C.) and the Department of Internal Medicine and Tygerberg Hospital (P.B.), University of Stellenbosch, Tygerberg, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingegerd","family":"O\u0308stman-Smith","sequence":"additional","affiliation":[{"name":"From the Departments of Cardiovascular Medicine (E.B., C.R., M.J.O., H.W.) and Paediatric Cardiology (I.O.S.), University of Oxford and John Radcliffe Hospital, Oxford, UK; the US\/MRC Centre for Molecular and Cellular Biology (J.C.M.S., V.A.C.) and the Department of Internal Medicine and Tygerberg Hospital (P.B.), University of Stellenbosch, Tygerberg, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugh","family":"Watkins","sequence":"additional","affiliation":[{"name":"From the Departments of Cardiovascular Medicine (E.B., C.R., M.J.O., H.W.) and Paediatric Cardiology (I.O.S.), University of Oxford and John Radcliffe Hospital, Oxford, UK; the US\/MRC Centre for Molecular and Cellular Biology (J.C.M.S., V.A.C.) and the Department of Internal Medicine and Tygerberg Hospital (P.B.), University of Stellenbosch, Tygerberg, South Africa."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199703133361107"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90274-I"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1295-434"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0596-63"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0897-379"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199504203321603"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI6460"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1221"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.83.6.580"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199204233261703"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.9.3864"},{"key":"e_1_3_3_12_2","unstructured":"Australian National Genomic Information Service National Health and Medical Research Council. 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