{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:30:59Z","timestamp":1791196259743,"version":"4.1.0"},"reference-count":52,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"11","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2001,6,8]]},"abstract":"<jats:p>\n            <jats:italic>Abstract<\/jats:italic>\n            \u2014Connexin43 (Cx43) is the principal connexin isoform in the mouse ventricle, where it is thought to provide electrical coupling between cells. Knocking out this gene results in anatomic malformations that nevertheless allow for survival through early neonatal life. We examined electrical wave propagation in the left (LV) and right (RV) ventricles of isolated Cx43 null mutated (Cx43\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            ), heterozygous (Cx43\n            <jats:sup>+\/<\/jats:sup>\n            <jats:sup>\u2212<\/jats:sup>\n            ), and wild-type (WT) embryos using high-resolution mapping of voltage-sensitive dye fluorescence. Consistent with the compensating presence of the other connexins, no reduction in propagation velocity was seen in Cx43\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            ventricles at postcoital day (dpc) 12.5 compared with WT or Cx43\n            <jats:sup>+\/<\/jats:sup>\n            <jats:sup>\u2212<\/jats:sup>\n            ventricles. A gross reduction in conduction velocity was seen in the RV at 15.5 dpc (in cm\/second, mean [1 SE confidence interval], WT 9.9 [8.7 to 11.2], Cx43\n            <jats:sup>+\/<\/jats:sup>\n            <jats:sup>\u2212<\/jats:sup>\n            9.9 [9.0 to 10.9], and Cx43\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            2.2 [1.8 to 2.7;\n            <jats:italic>P<\/jats:italic>\n            &lt;0.005]) and in both ventricles at 17.5 dpc (in RV, WT 8.4 [7.6 to 9.3], Cx43\n            <jats:sup>+\/<\/jats:sup>\n            <jats:sup>\u2212<\/jats:sup>\n            8.7 [8.1 to 9.3], and Cx43\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            1.1 [0.1 to 1.3;\n            <jats:italic>P<\/jats:italic>\n            &lt;0.005]; in LV, WT 10.1 [9.4 to 10.7], Cx43\n            <jats:sup>+\/<\/jats:sup>\n            <jats:sup>\u2212<\/jats:sup>\n            8.3 [7.8 to 8.9], and Cx43\n            <jats:sup>\u2212\/\u2212<\/jats:sup>\n            1.7 [1.3 to 2.1;\n            <jats:italic>P<\/jats:italic>\n            &lt;0.005]) corresponding with the downregulation of Cx40. Cx40 and Cx45 mRNAs were detectable in ventricular homogenates even at 17.5 dpc, probably accounting for the residual conduction function. Neonatal knockout hearts were arrhythmic in vivo as well as ex vivo. This study demonstrates the contribution of Cx43 to the electrical function of the developing mouse heart and the essential role of this gene in maintaining heart rhythm in postnatal life.\n          <\/jats:p>","DOI":"10.1161\/hh1101.091107","type":"journal-article","created":{"date-parts":[[2007,9,28]],"date-time":"2007-09-28T18:32:49Z","timestamp":1191004369000},"page":"1196-1202","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":115,"title":["Null Mutation of Connexin43 Causes Slow Propagation of Ventricular Activation in the Late Stages of Mouse Embryonic Development"],"prefix":"10.1161","volume":"88","author":[{"given":"Dhananjay","family":"Vaidya","sequence":"first","affiliation":[{"name":"From the Department of Pharmacology (D.V., H.S.T., M.D., G.E.M., J.J.) and Department of Microbiology and Immunology (S.M.T.), SUNY Upstate Medical University, Syracuse, NY, and Department of Biology (C.W.L.), University of Pennsylvania, Philadelphia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houman S.","family":"Tamaddon","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology (D.V., H.S.T., M.D., G.E.M., J.J.) and Department of Microbiology and Immunology (S.M.T.), SUNY Upstate Medical University, Syracuse, NY, and Department of Biology (C.W.L.), University of Pennsylvania, Philadelphia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia W.","family":"Lo","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology (D.V., H.S.T., M.D., G.E.M., J.J.) and Department of Microbiology and Immunology (S.M.T.), SUNY Upstate Medical University, Syracuse, NY, and Department of Biology (C.W.L.), University of Pennsylvania, Philadelphia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven M.","family":"Taffet","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology (D.V., H.S.T., M.D., G.E.M., J.J.) and Department of Microbiology and Immunology (S.M.T.), SUNY Upstate Medical University, Syracuse, NY, and Department of Biology (C.W.L.), University of Pennsylvania, Philadelphia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Delmar","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology (D.V., H.S.T., M.D., G.E.M., J.J.) and Department of Microbiology and Immunology (S.M.T.), SUNY Upstate Medical University, Syracuse, NY, and Department of Biology (C.W.L.), University of Pennsylvania, Philadelphia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory E.","family":"Morley","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology (D.V., H.S.T., M.D., G.E.M., J.J.) and Department of Microbiology and Immunology (S.M.T.), SUNY Upstate Medical University, Syracuse, NY, and Department of Biology (C.W.L.), University of Pennsylvania, Philadelphia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301","family":"Jalife","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology (D.V., H.S.T., M.D., G.E.M., J.J.) and Department of Microbiology and Immunology (S.M.T.), SUNY Upstate Medical University, Syracuse, NY, and Department of Biology (C.W.L.), University of Pennsylvania, Philadelphia, Pa."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1998.3972"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(96)00131-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1002040403"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.81.3.423"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199411000-00002"},{"key":"e_1_3_2_6_2","first-page":"267","volume":"191","year":"1995","unstructured":"Dahl E, Winterhager E, Traub O, Willecke K. Expression of gap junction genes, connexin40 and connexin43, during fetal mouse development. Anat Embryol. 1995;191:267\u2013278.","journal-title":"Anat Embryol"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6408(1999)24:1\/2<82::AID-DVG9>3.0.CO;2-1"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.71.2.1378358"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8167.1995.tb00357.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.73.2.8392450"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(94)90879-6"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.105.4.985"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7892609"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70113-7"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70114-9"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.16.3501"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.19.4179"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1520-6408(1996)18:3<234::AID-DVG4>3.0.CO;2-A"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.82.3.360"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2828(90)90061-6"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/355349a0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8167.1998.tb00897.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.79.5.957"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8167.1999.tb00192.x"},{"key":"e_1_3_2_25_2","first-page":"1077","volume":"13","year":"1997","unstructured":"Witkowski FX, Clark RB, Larsen TS, Melnikov A, Giles WR. Voltage-sensitive dye recordings of electrophysiological activation in a Langendorff-perfused mouse heart. Can J Cardiol. 1997;13:1077\u20131082.","journal-title":"Can J Cardiol"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1991.71.1.53"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.81.4.470"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.79.1.115"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00373887"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.87.10.929"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.85.2.174"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.79.2.247"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.98.9.912"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107411"},{"key":"e_1_3_2_35_2","first-page":"H1401","volume":"265","year":"1993","unstructured":"Grupp IL, Subramaniam A, Hewett TE, Robbins J, Grupp G. Comparison of normal, hypodynamic, and hyperdynamic mouse hearts using isolated work-performing heart preparations. Am J Physiol. 1993;265:H1401\u2013H1410.","journal-title":"Am J Physiol"},{"key":"e_1_3_2_36_2","unstructured":"Kaufman MH. The Atlas of Mouse Development. San Diego Calif: Academic Press; 1998."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8167.1996.tb00478.x"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.72.3.631"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2000.278.1.C73"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1540-8167.1999.tb00195.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.61.6.3677338"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.84.12.1365"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00690-4"},{"key":"e_1_3_2_44_2","unstructured":"Keener JP Panfilov AV. Three dimensional propagation in the heart: the effects of geometry and fiber orientation on propagation in myocardium. In: Zipes DP Jalife J eds. Cardiac Physiology: From Cell to Bedside. Philadelphia Pa: WB Saunders; 1995:335\u2013347."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.78.1.15"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.81.1.120"},{"key":"e_1_3_2_47_2","first-page":"H2088","volume":"270","year":"1996","unstructured":"Wang L, Duff HJ. Identification and characteristics of delayed rectifier K+ current in fetal mouse ventricular myocytes. Am J Physiol. 1996;270:H2088\u2013H2093.","journal-title":"Am J Physiol"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.87.4.316"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119367"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.97.7.686"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.86.12.1193"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1161\/res.81.5.727"}],"container-title":["Circulation Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/hh1101.091107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T02:12:49Z","timestamp":1715652769000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/hh1101.091107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,6,8]]},"references-count":52,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2001,6,8]]}},"alternative-id":["10.1161\/hh1101.091107"],"URL":"https:\/\/doi.org\/10.1161\/hh1101.091107","relation":{},"ISSN":["0009-7330","1524-4571"],"issn-type":[{"value":"0009-7330","type":"print"},{"value":"1524-4571","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,6,8]]},"assertion":[{"value":"2001-06-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}