{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T11:57:22Z","timestamp":1762430242646},"reference-count":24,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Circulation Research"],"published-print":{"date-parts":[[2001,9,14]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>Abstract\u2014<\/jats:italic>\n            <\/jats:bold>\n            Platelet-derived growth factor (PDGF) is thought to play a significant role in various models of vascular remodeling, particularly in the early process of vascular diseases. Its action is mediated by its specific receptor, the PDGF receptor. The PDGF \u03b1-receptor (PDGF\u03b1R) plays an important role in the growth and proliferation of vascular smooth muscle cells (VSMCs), and its gene expression is thought to be regulated by several potential transcriptional nuclear factors. However, the detailed mechanisms of tissue-specific transactivation of the PDGF\u03b1R gene in VSMCs remain to be clarified. We have previously demonstrated that the rat PDGF\u03b1R gene contains an enhancer core sequence for CCAAT\/enhancer-binding proteins (C\/EBPs) in its promoter region, and we have also suggested that C\/EBP-\u03b4 is the principal factor involved in the induction of tissue-specific transcriptional activity of the PDGF\u03b1R gene in VSMCs. To explore the definitive roles of C\/EBP-\u03b4 protein on PDGF\u03b1R gene transcription in VSMCs, we developed C\/EBP-\u03b4 transgenic rats by using a chimeric fusion gene of the mouse smooth muscle \u03b1-actin promoter and an entire coding region of rat C\/EBP-\u03b4 cDNA. This report describes the first successful targeted overexpression of C\/EBP-\u03b4 capable of inducing PDGF\u03b1R gene transcription and modifying cell proliferative activity to PDGFs. Targeted overexpression of C\/EBP-\u03b4 evokes high levels of PDGF\u03b1R gene expression, susceptibility to VSMC growth, and proliferation of VSMCs to PDGFs. The results obtained reveal evidence of a new role and new functional significance of C\/EBP-\u03b4 on VSMC growth via the PDGF\u03b1R during the process of vascular remodeling and atherosclerosis.\n          <\/jats:p>","DOI":"10.1161\/hh1801.096265","type":"journal-article","created":{"date-parts":[[2007,9,28]],"date-time":"2007-09-28T18:32:49Z","timestamp":1191004369000},"page":"503-508","update-policy":"http:\/\/dx.doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":25,"title":["Targeted Overexpression of CCAAT\/Enhancer-Binding Protein-\u03b4 Evokes Enhanced Gene Transcription of Platelet-Derived Growth Factor \u03b1-Receptor in Vascular Smooth Muscle Cells"],"prefix":"10.1161","volume":"89","author":[{"given":"Zhao-Hui","family":"Yang","sequence":"first","affiliation":[{"name":"From The Second Department of Internal Medicine, Ehime University School of Medicine, Ehime, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yutaka","family":"Kitami","sequence":"additional","affiliation":[{"name":"From The Second Department of Internal Medicine, Ehime University School of Medicine, Ehime, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasunori","family":"Takata","sequence":"additional","affiliation":[{"name":"From The Second Department of Internal Medicine, Ehime University School of Medicine, Ehime, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takafumi","family":"Okura","sequence":"additional","affiliation":[{"name":"From The Second Department of Internal Medicine, Ehime University School of Medicine, Ehime, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunio","family":"Hiwada","sequence":"additional","affiliation":[{"name":"From The Second Department of Internal Medicine, Ehime University School of Medicine, Ehime, Japan."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"276","reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.hyp.23.6.1132"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1992.tb05523.x"},{"key":"e_1_3_3_3_2","first-page":"1488","volume":"146","year":"1995","unstructured":"Lindner V, Reidy MA. 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