{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T09:21:05Z","timestamp":1789464065680,"version":"build-2803163510"},"reference-count":29,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"3","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Stroke"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:p>\n            <jats:bold>\n              <jats:italic>\n                <jats:bold>\n                  <jats:italic>Background and Purpose<\/jats:italic>\n                <\/jats:bold>\n                \u2014\n              <\/jats:italic>\n            <\/jats:bold>\n            Macrophage inflammatory protein (MIP)-1\u03b1 is a well-characterized monocyte chemoattractant; its role in regulating monocyte and microglial recruitment and activation in the injured neonatal brain is unknown. We evaluated the impact of acute hypoxic-ischemic (HI) brain injury on the expression of MIP-1\u03b1 in neonatal rat brain.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>\n                <jats:bold>\n                  <jats:italic>Methods<\/jats:italic>\n                <\/jats:bold>\n                \u2014\n              <\/jats:italic>\n            <\/jats:bold>\n            To elicit forebrain ischemic injury, 7-day-old (P7) rats underwent right carotid ligation, followed by 2.5 hours of 8% oxygen exposure. We used an enzyme-linked immunosorbent assay and immunohistochemistry to detect MIP-1\u03b1; double-labeling immunofluorescence assays were analyzed with confocal microscopy to identify cellular sources of MIP-1\u03b1. Immunocytochemistry assays were also used to detect 2 MIP-1\u03b1 receptors, CCR1 and CCR5.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>\n                <jats:bold>\n                  <jats:italic>Results<\/jats:italic>\n                <\/jats:bold>\n                \u2014\n              <\/jats:italic>\n            <\/jats:bold>\n            We found marked increases in tissue concentrations of MIP-1\u03b1 in the HI cerebral hemisphere, peaking from 8 to 72 hours after lesioning. Immunocytochemistry assays revealed that MIP-1\u03b1 was constitutively expressed in physiologically activated microglia; from 8 to 120 hours after lesioning, MIP-1\u03b1 immunoreactive monocytes and microglia accumulated in the lesion territory. In immunoreactive cells, MIP-1\u03b1 was diffusely distributed throughout the cytoplasm at early post-HI time intervals; by 72 hours, MIP-1\u03b1 immunoreactivity was typically concentrated adjacent to the nucleus, a pattern indicative of active MIP-1\u03b1 production. In P7 to P12 brain, many cells expressed MIP-1\u03b1 receptors; both CCR1 and CCR5 immunoreactivity were localized to endothelium and ependyma; CCR1-immunoreactive astrocytes and neurons and CCR5-immunoreactive microglia were also identified.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>\n              <jats:italic>\n                <jats:bold>\n                  <jats:italic>Conclusions<\/jats:italic>\n                <\/jats:bold>\n                \u2014\n              <\/jats:italic>\n            <\/jats:bold>\n            These data implicate MIP-1\u03b1 as a mediator of the complex and sustained inflammatory response initiated by perinatal HI braininjury.\n          <\/jats:p>","DOI":"10.1161\/hs0302.103740","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T23:16:47Z","timestamp":1027898207000},"page":"795-801","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":129,"title":["Hypoxic-Ischemic Injury Induces Macrophage Inflammatory Protein-1\u03b1 Expression in Immature Rat Brain"],"prefix":"10.1161","volume":"33","author":[{"given":"Rita M.","family":"Cowell","sequence":"first","affiliation":[{"name":"From the Departments of Pediatrics and Neurology (H.X., F.S.S.), Neuroscience Program (R.M.C., F.S.S.), and Medical School (J.M.G., F.S.S.), University of Michigan, Ann Arbor, Mich."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Xu","sequence":"additional","affiliation":[{"name":"From the Departments of Pediatrics and Neurology (H.X., F.S.S.), Neuroscience Program (R.M.C., F.S.S.), and Medical School (J.M.G., F.S.S.), University of Michigan, Ann Arbor, Mich."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John M.","family":"Galasso","sequence":"additional","affiliation":[{"name":"From the Departments of Pediatrics and Neurology (H.X., F.S.S.), Neuroscience Program (R.M.C., F.S.S.), and Medical School (J.M.G., F.S.S.), University of Michigan, Ann Arbor, Mich."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faye S.","family":"Silverstein","sequence":"additional","affiliation":[{"name":"From the Departments of Pediatrics and Neurology (H.X., F.S.S.), Neuroscience Program (R.M.C., F.S.S.), and Medical School (J.M.G., F.S.S.), University of Michigan, Ann Arbor, Mich."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-199908000-00001"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1996.0059"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.23.13496"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410440413"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/str.26.6.1093"},{"key":"e_1_3_3_6_2","first-page":"759","volume":"17","year":"1997","unstructured":"Ivacko J, Szaflarski J, Malinak C, Flory C, Warren JS, Silverstein FS. Hypoxic-ischemic injury induces monocyte chemoattractant protein-1 expression in neonatal rat brain. J Cereb Blood Flow Metab. 1997; 17: 759\u2013770.","journal-title":"J Cereb Blood Flow Metab"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199904010-00008"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199601000-00001"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1994.1047"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(98)00187-8"},{"key":"e_1_3_3_11_2","doi-asserted-by":"crossref","first-page":"5003","DOI":"10.4049\/jimmunol.155.10.5003","volume":"155","year":"1995","unstructured":"Karpus WJ, Lukacs NW, McRae BL, Strieter RM, Kunkel SL, Miller SD. An important role for the chemokine macrophage inflammatory protein-1 alpha in the pathogenesis of the T cell-mediated autoimmune disease, experimental autoimmune encephalomyelitis. J Immunol. 1995; 155: 5003\u201310.","journal-title":"J Immunol"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(94)00138-E"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(97)00338-8"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(98)00295-4"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.163.3.1636"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19980701)53:1<16::AID-JNR3>3.0.CO;2-M"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0751898.x"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(99)00096-X"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(200003)30:1<1::AID-GLIA1>3.0.CO;2-Q"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.2000.7466"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/nbdi.2000.0371"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(86)90310-8"},{"key":"e_1_3_3_23_2","first-page":"69","volume":"27","year":"1992","unstructured":"Andersson J, Nagy S, Bjork L, Abrams J, Holm S, Andersson U. Bacterial toxin-induced cytokine production studied at the single-cell level. Immunol Rev. 1992; 27: 69\u201394.","journal-title":"Immunol Rev"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(98)00171-9"},{"key":"e_1_3_3_25_2","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.4049\/jimmunol.160.3.1449","volume":"160","year":"1998","unstructured":"McManus CM, Brosnan CF, Berman JW. Cytokine induction of MIP-1alpha and MIP-1beta in human fetal microglia. J Immunol. 1998; 160: 1449\u20131455.","journal-title":"J Immunol"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.162.3.1701"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2000.tb00248.x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65752-5"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19981001)71:1<36::AID-JCB4>3.0.CO;2-2"}],"container-title":["Stroke"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/hs0302.103740","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T02:14:33Z","timestamp":1715652873000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/hs0302.103740"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,3]]},"references-count":29,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,3]]}},"alternative-id":["10.1161\/hs0302.103740"],"URL":"https:\/\/doi.org\/10.1161\/hs0302.103740","relation":{},"ISSN":["0039-2499","1524-4628"],"issn-type":[{"value":"0039-2499","type":"print"},{"value":"1524-4628","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,3]]},"assertion":[{"value":"2001-09-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-11-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}