{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T23:20:48Z","timestamp":1791069648799,"version":"4.1.0"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2001,10]]},"abstract":"<jats:p>5-Hydroxytryptamine<jats:sub>1B<\/jats:sub>(5-HT<jats:sub>1B<\/jats:sub>) receptors have been implicated in mediating arterial contraction to 5-HT. Additionally, the 5-HT<jats:sub>1B<\/jats:sub>receptor has been reported to be \u201cunmasked\u201d by depolarizing stimuli. We hypothesized that 5-HT<jats:sub>1B<\/jats:sub>receptors in arteries from hypertensive animals, arteries reported to have a depolarized resting membrane potential in smooth muscle cells, are unmasked and participate in the supersensitivity observed to 5-HT in hypertension. We used the isolated tissue bath apparatus and examined the response of superior mesenteric arteries from normotensive sham and hypertensive deoxycorticosterone acetate (DOCA)\u2013salt rats. The 5-HT<jats:sub>1B<\/jats:sub>agonists CP93129 and sumatriptan (10<jats:sup>\u22129<\/jats:sup>to 10<jats:sup>\u22125<\/jats:sup>mol\/L) caused a maximal contraction (50\u00b112% of phenylephrine [10<jats:sup>\u22125<\/jats:sup>mol\/L] contraction) in arteries from DOCA-salt rats; no contraction was observed in arteries from normotensive rats. The 5-HT<jats:sub>1B<\/jats:sub>receptor antagonist GR55562 (100 nmol\/L) inhibited both the 5-HT\u2013 (4-fold rightward shift) and CP93129-induced (11-fold rightward shift) contractions in mesenteric arteries from hypertensive DOCA-salt rats. In other experiments, arteries from normotensive rats were incubated with 15 mmol\/L KCl, as a depolarizing stimulus, and then exposed to 5-HT and CP93129. In the presence of KCl, a small leftward shift to 5-HT was observed. However, the presence of a depolarizing stimulus was unable to produce changes in the 5-HT maximal response to resemble that of arteries from DOCA-salt rats, nor was contraction to CP93129 observed. These data support the conclusions that 5-HT<jats:sub>1B<\/jats:sub>receptors mediate contraction in mesenteric arteries from hypertensive rats and that this enhanced response to 5-HT is not due to membrane depolarization alone.<\/jats:p>","DOI":"10.1161\/hy1001.091779","type":"journal-article","created":{"date-parts":[[2007,9,28]],"date-time":"2007-09-28T18:37:17Z","timestamp":1191004637000},"page":"891-895","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":16,"title":["Enhanced Contraction to 5-Hydroxytryptamine Is Not Due to \u201cUnmasking\u201d of 5-Hydroxytryptamine<sub>1B<\/sub>Receptors in the Mesenteric Artery of the Deoxycorticosterone Acetate\u2013Salt Rat"],"prefix":"10.1161","volume":"38","author":[{"given":"Amy K.L.","family":"Banes","sequence":"first","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Michigan State University, East Lansing."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie W.","family":"Watts","sequence":"additional","affiliation":[{"name":"From the Department of Pharmacology and Toxicology, Michigan State University, East Lansing."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-199816060-00012"},{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1254\/jjp.44.502"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-8673.1995.tb00403.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/hyp.26.6.1056"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702841"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1996.tb15760.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(99)00114-4"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702773"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702684"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1016\/S0022-3565(24)38143-1","volume":"289","year":"1999","unstructured":"Smith JR, Kim C, Kim H, Purdy RE. 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