{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:52:06Z","timestamp":1785851526001,"version":"3.56.0"},"reference-count":46,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:sec>\n            <jats:title>Background:<\/jats:title>\n            <jats:p>\n              Vascular endothelium and smooth muscle work together to keep the balance of vasomotor tone and jointly maintain vascular homeostasis. Ca\n              <jats:sup>2+<\/jats:sup>\n              -permeable ion channel TRPV4 (transient receptor potential vanilloid family member 4) in endothelial cells regulates endothelium-dependent vasodilation and contraction in various states. However, how vascular smooth muscle cell TRPV4 (TRPV4\n              <jats:sub>SMC<\/jats:sub>\n              ) contributes to vascular function and blood pressure regulation in physiological and pathologically obese condition has not been fully studied.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods:<\/jats:title>\n            <jats:p>\n              We generated smooth muscle TRPV4-deficient mice and developed diet-induced obese mice model and analyzed the role of TRPV4\n              <jats:sub>SMC<\/jats:sub>\n              in intracellular Ca\n              <jats:sup>2+<\/jats:sup>\n              ([Ca\n              <jats:sup>2+<\/jats:sup>\n              ]\n              <jats:sub>i<\/jats:sub>\n              ) regulation and vasoconstriction. Vasomotor changes of mouse mesenteric artery were measured by wire, and pressure myography. [Ca\n              <jats:sup>2+<\/jats:sup>\n              ]\n              <jats:sub>i<\/jats:sub>\n              were measured by fluo-4 staining. Blood pressure was recorded by telemetric device.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results:<\/jats:title>\n            <jats:p>\n              Vascular TRPV4\n              <jats:sub>SMC<\/jats:sub>\n              played different roles in regulating vasomotor tone than endothelial TRPV4 due to their different features of [Ca\n              <jats:sup>2+<\/jats:sup>\n              ]\n              <jats:sub>i<\/jats:sub>\n              regulation. Loss of TRPV4\n              <jats:sub>SMC<\/jats:sub>\n              attenuated U46619- and phenylephrine-induced contraction, suggesting its involvement in regulating vascular contractility. Mesenteric arteries from obese mice showed SMC hyperplasia, suggesting an increased level of TRPV4\n              <jats:sub>SMC<\/jats:sub>\n              . Loss of TRPV4\n              <jats:sub>SMC<\/jats:sub>\n              did not influence the development of obesity but protected mice from obesity-induced vasoconstriction and hypertension. In arteries deficient in SMC TRPV4, SMCs F-actin polymerization and RhoA dephosphorylation were attenuated under contractile stimuli. Moreover, SMC-dependent vasoconstriction was inhibited in human resistance arteries with TRPV4 inhibitor application.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions:<\/jats:title>\n            <jats:p>\n              Our data identify TRPV4\n              <jats:sub>SMC<\/jats:sub>\n              as a regulator of vascular contraction in both physiological states and pathologically obese mice. TRPV4\n              <jats:sub>SMC<\/jats:sub>\n              contributes to the ontogeny of vasoconstriction and hypertension induced by TRPV4\n              <jats:sub>SMC<\/jats:sub>\n              over-expression in obese mice mesenteric artery.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/hypertensionaha.122.20109","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T10:00:42Z","timestamp":1676541642000},"page":"757-770","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":35,"title":["Vascular Smooth Muscle TRPV4 (Transient Receptor Potential Vanilloid Family Member 4) Channels Regulate Vasoconstriction and Blood Pressure in Obesity"],"prefix":"10.1161","volume":"80","author":[{"given":"Yifei","family":"Zhu","sequence":"first","affiliation":[{"name":"Wuxi School of Medicine, Jiangnan University, Wuxi, China (Y.Z., Y.C., S.W., L.F., F.Y., X.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2158-305X","authenticated-orcid":true,"given":"Yuan","family":"Chu","sequence":"additional","affiliation":[{"name":"Wuxi School of Medicine, Jiangnan University, Wuxi, China (Y.Z., Y.C., S.W., L.F., F.Y., X.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Wuxi School of Medicine, Jiangnan University, Wuxi, China (Y.Z., Y.C., S.W., L.F., F.Y., X.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junjian","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, Affiliated Hospital of Jiangnan University, Wuxi, China (J.T., H.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6146-5328","authenticated-orcid":true,"given":"Hu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Vascular Surgery, Affiliated Hospital of Jiangnan University, Wuxi, China (J.T., H.L.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Feng","sequence":"additional","affiliation":[{"name":"Wuxi School of Medicine, Jiangnan University, Wuxi, China (Y.Z., Y.C., S.W., L.F., F.Y., X.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Yu","sequence":"additional","affiliation":[{"name":"Wuxi School of Medicine, Jiangnan University, Wuxi, China (Y.Z., Y.C., S.W., L.F., F.Y., X.M.)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8525-068X","authenticated-orcid":true,"given":"Xin","family":"Ma","sequence":"additional","affiliation":[{"name":"Wuxi School of 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