{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T18:59:28Z","timestamp":1774465168695,"version":"3.50.1"},"reference-count":27,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["Hypertension"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND:<\/jats:title>\n                    <jats:p>A hypertensive disorder of pregnancy is associated with a higher risk of cardiovascular disease later in life, but the potential mechanistic links are unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS:<\/jats:title>\n                    <jats:p>We recruited 2 groups of women, 1 during pregnancy and another at least 2 years after delivery. Cases had a hypertensive disorder of pregnancy, and controls had a normotensive pregnancy. The pregnancy cohort had study visits antepartum and postpartum; the mid-life group made a single study visit. We assayed 7288 plasma proteins, applied machine learning to identify proteomics signatures at each time point, and performed enrichment analyses to identify relevant biological pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS:<\/jats:title>\n                    <jats:p>The pregnancy cohort (58 cases and 46 controls) had a mean age of 33.8 years, and the mid-life group (71 cases and 74 controls) had a mean age of 40.8 years. Protein levels differed significantly between cases and controls at each time point: 6233 antepartum, 189 postpartum, and 224 in mid-life. The postpartum protein signature discriminated well between cases and controls (c-index=0.78), and it also discriminated well in the independent mid-life samples (c-index=0.72). Pathway analyses identified differences in the complement and coagulation cascades that persisted across the antepartum, postpartum, and mid-life samples. The 28 proteins present in both the postpartum and mid-life signatures included 5 complement factors (3, B, H, H-related-1, and C1r-subcomponent-like) and coagulation factor IX.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS:<\/jats:title>\n                    <jats:p>Differences in protein expression persist for years after a hypertensive disorder of pregnancy. The consistent differences in the complement and coagulation pathways may contribute to the increased risk of later life cardiovascular disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1161\/hypertensionaha.124.24490","type":"journal-article","created":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T05:00:35Z","timestamp":1740114035000},"page":"872-882","update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":6,"title":["Persistence of a Proteomic Signature After a Hypertensive Disorder of Pregnancy"],"prefix":"10.1161","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4686-9441","authenticated-orcid":true,"given":"Mark A.","family":"Hlatky","sequence":"first","affiliation":[{"name":"Departments of Anesthesia, Health Policy, Medicine, Obstetrics and Gynecology, and Pediatrics, Stanford University School of Medicine; Department of Epidemiology Research, Statens Serum Institut; Danish Cancer Institute, and Department of Clinical Medicine, University of Copenhagen, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3486-8856","authenticated-orcid":true,"given":"Chi-Hung","family":"Shu","sequence":"additional","affiliation":[{"name":"Departments of Anesthesia, Health Policy, Medicine, Obstetrics and Gynecology, and Pediatrics, Stanford University School of Medicine; Department of Epidemiology Research, Statens Serum Institut; Danish Cancer Institute, and Department of Clinical Medicine, University of Copenhagen, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David K.","family":"Stevenson","sequence":"additional","affiliation":[{"name":"Departments of Anesthesia, Health Policy, Medicine, Obstetrics and Gynecology, and Pediatrics, Stanford University School of Medicine; Department of Epidemiology Research, Statens Serum Institut; Danish Cancer Institute, and Department of Clinical Medicine, University of Copenhagen, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary M.","family":"Shaw","sequence":"additional","affiliation":[{"name":"Departments of Anesthesia, Health Policy, Medicine, Obstetrics and Gynecology, and Pediatrics, Stanford University School of Medicine; Department of Epidemiology Research, Statens Serum Institut; Danish Cancer Institute, and Department of Clinical Medicine, University of Copenhagen, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0284-6996","authenticated-orcid":true,"given":"Marcia L.","family":"Stefanick","sequence":"additional","affiliation":[{"name":"Departments of Anesthesia, Health Policy, Medicine, Obstetrics and Gynecology, and Pediatrics, Stanford University School of Medicine; Department of Epidemiology Research, Statens Serum Institut; Danish Cancer Institute, and Department of Clinical Medicine, University of Copenhagen, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6849-9985","authenticated-orcid":true,"given":"Heather A.","family":"Boyd","sequence":"additional","affiliation":[{"name":"Departments of Anesthesia, Health Policy, Medicine, Obstetrics and Gynecology, and Pediatrics, Stanford University School of Medicine; Department of Epidemiology Research, Statens Serum Institut; Danish Cancer Institute, and Department of Clinical Medicine, University of Copenhagen, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8264-6785","authenticated-orcid":true,"given":"Mads","family":"Melbye","sequence":"additional","affiliation":[{"name":"Departments of Anesthesia, Health Policy, Medicine, Obstetrics and Gynecology, and Pediatrics, Stanford University School of Medicine; Department of Epidemiology Research, Statens Serum Institut; Danish Cancer Institute, and Department of Clinical Medicine, University of Copenhagen, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1581-5053","authenticated-orcid":true,"given":"Xi Du","family":"Plummer","sequence":"additional","affiliation":[{"name":"Departments of Anesthesia, Health Policy, Medicine, Obstetrics and Gynecology, and Pediatrics, Stanford University School of Medicine; Department of Epidemiology Research, Statens Serum Institut; Danish Cancer Institute, and Department of Clinical Medicine, University of Copenhagen, Denmark."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oshra","family":"Sedan","sequence":"additional","affiliation":[{"name":"Departments of Anesthesia, Health Policy, Medicine, Obstetrics and Gynecology, and Pediatrics, Stanford University School of Medicine; Department of Epidemiology Research, Statens Serum Institut; 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