{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T18:58:04Z","timestamp":1774551484397,"version":"3.50.1"},"reference-count":32,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"6","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2013,11,18]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Human ascending thoracic aortic aneurysms (\n              <jats:styled-content style=\"fixed-case\">ATAAs<\/jats:styled-content>\n              ) are life threatening and constitute a leading cause of mortality in the\n              <jats:styled-content style=\"fixed-case\">United States<\/jats:styled-content>\n              . Previously, we demonstrated that collagens \u03b12(V) and \u03b11(XI)\n              <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>\n              and protein expression levels are significantly increased in\n              <jats:styled-content style=\"fixed-case\">ATAAs<\/jats:styled-content>\n              .\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              In this report, the authors extended these preliminary studies using high\u2010throughput proteomic analysis to identify additional biomarkers for use in whole blood real\u2010time\n              <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\n              \u2010\n              <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n              analysis to allow for the identification of\n              <jats:styled-content style=\"fixed-case\">ATAAs<\/jats:styled-content>\n              before dissection or rupture. Human\n              <jats:styled-content style=\"fixed-case\">ATAA<\/jats:styled-content>\n              samples were obtained from male and female patients aged 65\u00b114\u00a0years. Both bicuspid and tricuspid aortic valve patients were included and compared with nonaneurysmal aortas (mean diameter 2.3\u00a0cm). Five biomarkers were identified as being suitable for detection and identification of\n              <jats:styled-content style=\"fixed-case\">ATAAs<\/jats:styled-content>\n              using q\n              <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\n              \u2010\n              <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n              analysis of whole blood. Analysis of 41 samples (19 small, 13 medium\u2010sized, and 9 large\n              <jats:styled-content style=\"fixed-case\">ATAAs<\/jats:styled-content>\n              ) demonstrated the overexpression of 3 of these transcript biomarkers correctly identified 79.4% of patients with\n              <jats:styled-content style=\"fixed-case\">ATAA<\/jats:styled-content>\n              of \u22654.0\u00a0cm (\n              <jats:italic>P<\/jats:italic>\n              &lt;0.001, sensitivity 0.79,\n              <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\n              =0.62 to 0.91; specificity 1.00, 95%\n              <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\n              =0.42 to 1.00).\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              A preliminary transcript biomarker panel for the identification of\n              <jats:styled-content style=\"fixed-case\">ATAAs<\/jats:styled-content>\n              using whole blood q\n              <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\n              \u2010\n              <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n              analysis in men and women is presented.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.113.000138","type":"journal-article","created":{"date-parts":[[2013,11,15]],"date-time":"2013-11-15T03:19:24Z","timestamp":1384485564000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":22,"title":["Preliminary Biomarkers for Identification of Human Ascending Thoracic Aortic Aneurysm"],"prefix":"10.1161","volume":"2","author":[{"given":"Kendra M.","family":"Black","sequence":"first","affiliation":[{"name":"Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center, Boston, MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Masuzawa","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center, Boston, MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert C.","family":"Hagberg","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center, Boston, MA"},{"name":"Harvard Medical School, Boston, MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamal R.","family":"Khabbaz","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center, Boston, MA"},{"name":"Harvard Medical School, Boston, MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary E.","family":"Trovato","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center, Boston, MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verna M.","family":"Rettagliati","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Beth Israel Deaconess Medical Center, Boston, MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manoj K.","family":"Bhasin","sequence":"additional","affiliation":[{"name":"Beth Israel Deaconess Medical Center Genomics Center, Boston, MA"},{"name":"Harvard Medical School, Boston, MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon T.","family":"Dillon","sequence":"additional","affiliation":[{"name":"Beth Israel Deaconess Medical Center Genomics Center, Boston, MA"},{"name":"Harvard Medical School, Boston, MA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Towia A.","family":"Libermann","sequence":"additional","affiliation":[{"name":"Beth Israel Deaconess Medical Center Genomics Center, Boston, MA"},{"name":"Harvard Medical School, Boston, 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