{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:37:59Z","timestamp":1791315479594,"version":"4.3.1"},"reference-count":50,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2013,8,22]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">Endothelial cell responses during inflammation are heterogeneous and key for selectivity in how leukocytes hone in on specific sites and why vascular diseases are highly bed specific. However, mechanisms for this specificity remain unclear.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Here, we exposed human endothelial cells isolated from 5 systemic arterial beds from 1 donor (to overcome donor\u2010to\u2010donor genetic\/epigenetic differences), the umbilical vein, and pulmonary microvasculature to\n              <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\n              \u2010\u03b1,\n              <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\n              , and\n              <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n              \u20101\u03b2 and assessed acute (\n              <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>\n              1\/2 and p65) and chronic (\n              <jats:styled-content style=\"fixed-case\">ICAM<\/jats:styled-content>\n              \u20101,\n              <jats:styled-content style=\"fixed-case\">VCAM<\/jats:styled-content>\n              \u20101 total and surface expression) signaling responses and assessed changes in surface\n              <jats:italic>N<\/jats:italic>\n              \u2010glycans and monocyte adhesion. Significant diversity in responses was evident by disparate changes in\n              <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content>\n              1\/2 and p65\n              <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\n              \u2010\u03baB phosphorylation, which varied up to 5\u2010fold between different cells and in temporal and magnitude differences in\n              <jats:styled-content style=\"fixed-case\">ICAM<\/jats:styled-content>\n              \u20101 and\n              <jats:styled-content style=\"fixed-case\">VCAM<\/jats:styled-content>\n              \u20101 expression (maximal\n              <jats:styled-content style=\"fixed-case\">VCAM<\/jats:styled-content>\n              \u20101 induction typically being observed by 4\u00a0hours, whereas\n              <jats:styled-content style=\"fixed-case\">ICAM<\/jats:styled-content>\n              \u20101 expression was increased further at 24\u00a0hours relative to 4\u00a0hours).\n              <jats:italic>N<\/jats:italic>\n              \u2010glycan profiles both basally and with stimulation were also bed specific, with hypoglycosylated\n              <jats:italic>N<\/jats:italic>\n              \u2010glycans correlating with increased\n              <jats:styled-content style=\"fixed-case\">THP<\/jats:styled-content>\n              \u20101 monocyte adhesion. Differences in surface\n              <jats:italic>N<\/jats:italic>\n              \u2010glycan expression tracked with dynamic up\u2010 or downregulation of \u03b1\u2010mannosidase activity during inflammation.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              These results demonstrate a critical role for the vascular bed of origin in controlling endothelial responses and function to inflammatory stimuli and suggest that bed\u2010specific expression of\n              <jats:italic>N<\/jats:italic>\n              \u2010linked sugars may provide a signature for select leukocyte recruitment.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1161\/jaha.113.000263","type":"journal-article","created":{"date-parts":[[2013,7,31]],"date-time":"2013-07-31T06:15:12Z","timestamp":1375251312000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":63,"title":["Heterogenic Endothelial Responses to Inflammation: Role for Differential\n            <i>N<\/i>\n            \u2010Glycosylation and Vascular Bed of Origin"],"prefix":"10.1161","volume":"2","author":[{"given":"David W.","family":"Scott","sequence":"first","affiliation":[{"name":"Department of Pathology and Comprehensive Cardiovascular Center, University of Alabama at Birmingham, Birmingham, AL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew O.","family":"Vallejo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cardiovascular Center, University of Alabama at Birmingham, Birmingham, AL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh P.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cardiovascular Center, University of Alabama at Birmingham, Birmingham, AL"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2156"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2012.11.006"},{"key":"e_1_3_3_4_2","first-page":"637","article-title":"Specification of arterial, venous, and lymphatic endothelial cells during embryonic development","volume":"25","author":"Kume T","year":"2010","unstructured":"Kume T. 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