{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T13:36:51Z","timestamp":1790516211762,"version":"4.1.0"},"reference-count":48,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"4","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2013,8,22]]},"abstract":"<jats:sec xml:lang=\"en\">\n            <jats:title>Background<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              A high consumption of omega\u20103 long\u2010chain polyunsaturated fatty acids, and particularly docosahexaenoic acid (\n              <jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content>\n              ), has been suggested to reduce the risk of cardiovascular disease (\n              <jats:styled-content style=\"fixed-case\">CVD<\/jats:styled-content>\n              ). However, while\n              <jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content>\n              supplementation may have benefits for secondary prevention, few studies have investigated the role of\n              <jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content>\n              in the primary prevention of\n              <jats:styled-content style=\"fixed-case\">CVD<\/jats:styled-content>\n              . Here, we tested the hypothesis that\n              <jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content>\n              supplementation improves endothelial function and risk factors for\n              <jats:styled-content style=\"fixed-case\">CVD<\/jats:styled-content>\n              .\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Methods and Results<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              Healthy volunteers (n=328), aged 18 to 37 years, were randomly assigned to 1.6\u00a0g\n              <jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content>\n              \/day (from a microalgae source) together with 2.4\u00a0g\/day carrier oil (index group) or to 4.0\u00a0g\/day olive oil (control) (both given in eight 500\u2010mg capsules\/day for 16\u00a0weeks). Flow\u2010mediated endothelium\u2010dependent vasodilation (\n              <jats:styled-content style=\"fixed-case\">FMD<\/jats:styled-content>\n              ) of the brachial artery (primary outcome) was measured before and after the intervention (n=268) using high\u2010resolution vascular ultrasound.\n              <jats:styled-content style=\"fixed-case\">FMD<\/jats:styled-content>\n              was the same in both groups at randomization (mean,\n              <jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>\n              ; 0.27, 0.1\u00a0mm), but postintervention was higher in the control group (0.29, 0.1\u00a0mm) compared with the\n              <jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content>\n              \u2010supplemented group (0.26, 0.1\u00a0mm; mean difference \u22120.03\u00a0mm; 95%\n              <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\n              \u22120.005 to \u22120.06\u00a0mm;\n              <jats:italic>P<\/jats:italic>\n              =0.02). Of other outcomes, only triglyceride (mean difference \u221228%, 95%\n              <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>\n              \u221240% to \u221215%;\n              <jats:italic>P<\/jats:italic>\n              &lt;0.0001) and very low\u2010density lipoprotein concentrations were significant lower in\n              <jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content>\n              \u2010supplemented individuals compared with controls.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              <jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content>\n              supplementation did not improve endothelial function in healthy, young adults. Nevertheless, lower triglyceride concentrations with\n              <jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content>\n              supplementation was consistent with previous reports and could have benefits for the prevention of\n              <jats:styled-content style=\"fixed-case\">CVD<\/jats:styled-content>\n              .\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec xml:lang=\"en\">\n            <jats:title>Clinical Trial Registration Information<\/jats:title>\n            <jats:p xml:lang=\"en\">\n              URL:\n              <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"http:\/\/www.controlled-trials.com\/\">http:\/\/www.controlled-trials.com\/<\/jats:ext-link>\n              Unique identifier:\n              <jats:styled-content style=\"fixed-case\">ISRCTN<\/jats:styled-content>\n              no: 19987575.\n            <\/jats:p>\n          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