{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T12:23:32Z","timestamp":1783167812563,"version":"3.54.6"},"reference-count":23,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"5","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2015,5]]},"abstract":"\n Background<\/jats:title>\n \n Only 2% of mothers positive for anti\u2010\n SSA<\/jats:styled-content>\n \/Ro (Ro) antibodies have children with congenital heart block (\n CHB<\/jats:styled-content>\n ). This study aimed to determine whether reactivity with p305, an epitope within the \u03b1\n 1G<\/jats:sub>\n T\u2010type calcium channel, confers added risk over anti\u2010Ro antibodies.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results<\/jats:title>\n \n Using sera from anti\u2010Ro\u2010exposed pregnancies resulting in offspring with\n CHB<\/jats:styled-content>\n , no disease but\n CHB<\/jats:styled-content>\n \u2010sibling, and no disease and no\n CHB<\/jats:styled-content>\n \u2010sibling,\u00a0as well as disease (lupus without anti\u2010Ro) and healthy controls, reactivities were determined for binding to Ro60, p305, and an epitope within Ro60, p133\u2010Ro60, which shares structural properties with p305, including key amino acids and an \u03b1\u2010helical structure. Candidate peptides were further evaluated in an in\u00a0vitro model that assessed the binding of maternal antibodies to apoptotic cells. In anti\u2010Ro\u2010positive mothers, anti\u2010p305 autoantibodies (>3\u00a0\n SD<\/jats:styled-content>\n above healthy controls) were detected in 3\/59 (5%)\n CHB<\/jats:styled-content>\n pregnancies, 4\/30 (13%) unaffected pregnancies with a\n CHB<\/jats:styled-content>\n \u2010sibling, and 0\/42 (0%) of unaffected pregnancies with no\n CHB<\/jats:styled-content>\n \u2010sibling. For umbilical bloods (61\n CHB<\/jats:styled-content>\n , 41 healthy with\n CHB<\/jats:styled-content>\n sibling), no association of anti\u2010p305 with outcome was detected; however, overall levels of anti\u2010p305 were elevated compared to mothers during pregnancy in all groups studied. For anti\u2010p133\u2010Ro60, reactivity paralleled that of anti\u2010p305. In the screen employing apoptotic cells, p133\u2010Ro60, but not p305, significantly attenuated the binding of immunoglobulin G isolated from a mother whose child had\n CHB<\/jats:styled-content>\n (42.1% reduced to 13.9%, absence\/presence of p133\u2010Ro60, respectively,\n P<\/jats:italic>\n <0.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n These data suggest that anti\u2010p305 is not a robust maternal marker for assessing increased risk of\n CHB<\/jats:styled-content>\n during an anti\u2010\n SSA<\/jats:styled-content>\n \/Ro pregnancy.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/jaha.115.001836","type":"journal-article","created":{"date-parts":[[2015,5,21]],"date-time":"2015-05-21T02:11:14Z","timestamp":1432174274000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":9,"title":["Reactivity to the p305 Epitope of the \u03b1\n 1G<\/sub>\n T\u2010Type Calcium Channel and Autoimmune\u2010Associated Congenital Heart Block"],"prefix":"10.1161","volume":"4","author":[{"given":"Androo J.","family":"Markham","sequence":"first","affiliation":[{"name":"Department of Medicine New York University School of Medicine New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara E.","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Department of Medicine New York University School of Medicine New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane E.","family":"Salmon","sequence":"additional","affiliation":[{"name":"Division of Rheumatology Hospital for Special Surgery New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilnelly","family":"Martinez\u2010Ortiz","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Pharmacology New York University School of Medicine New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J.","family":"Cardozo","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Pharmacology New York University School of Medicine New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Clancy","sequence":"additional","affiliation":[{"name":"Department of Medicine New York University School of Medicine New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill P.","family":"Buyon","sequence":"additional","affiliation":[{"name":"Department of Medicine New York University School of Medicine New York NY"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-374994-9.10031-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114208"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.707661"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.56.1.82"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200108)44:8<1832::AID-ART320>3.0.CO;2-C"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.9.5061"},{"key":"e_1_3_2_8_2","first-page":"2413","article-title":"Impaired clearance of apoptotic cardiocytes is linked to anti\u2010SSA\/Ro and \u2010SSB\/La antibodies in the pathogenesis of congenital heart block","volume":"116","author":"Clancy RM","year":"2006","unstructured":"Clancy RM, Neufing PJ, Zheng P, O'Mahony M, Nimmerjahn F, Gordon TP, Buyon JP. Impaired clearance of apoptotic cardiocytes is linked to anti\u2010SSA\/Ro and \u2010SSB\/La antibodies in the pathogenesis of congenital heart block. J Clin Invest. 2006;116:2413\u20132422.","journal-title":"J Clin Invest"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199807000-00002"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0072668"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(98)00161-2"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.34402"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1287"},{"key":"e_1_3_2_14_2","first-page":"1","article-title":"Assessment of immunologically relevant dynamic tertiary structural features of the HIV\u20101\u00a0V3 loop crown R2 sequence by ab\u00a0initio folding","volume":"43","author":"Almond D","year":"2010","unstructured":"Almond D, Cardozo T. Assessment of immunologically relevant dynamic tertiary structural features of the HIV\u20101\u00a0V3 loop crown R2 sequence by ab\u00a0initio folding. J Vis Exp. 2010;43:1\u20133.","journal-title":"J Vis Exp"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.2009.0254"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/803535"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.11430"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150191023"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199902)42:2<199::AID-ANR1>3.0.CO;2-1"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1177\/0961203307085115"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202849"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2007.03331.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24768"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/ker515"}],"container-title":["Journal of the American Heart Association"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.ahajournals.org\/doi\/full\/10.1161\/JAHA.115.001836","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T10:35:25Z","timestamp":1715596525000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ahajournals.org\/doi\/10.1161\/JAHA.115.001836"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1161\/JAHA.115.001836"],"URL":"https:\/\/doi.org\/10.1161\/jaha.115.001836","relation":{},"ISSN":["2047-9980"],"issn-type":[{"value":"2047-9980","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5]]},"assertion":[{"value":"2015-01-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-04-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-05-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e001836"}}