{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T19:20:49Z","timestamp":1790364049543,"version":"4.1.0"},"reference-count":28,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"8","content-domain":{"domain":["www.ahajournals.org"],"crossmark-restriction":true},"short-container-title":["JAHA"],"published-print":{"date-parts":[[2015,8,25]]},"abstract":"\n Background<\/jats:title>\n \n Well\u2010defined inflammation, proliferation, and maturation phases orchestrate the remodeling of the injured myocardium after myocardial infarction (\n MI<\/jats:styled-content>\n ) by controlling the formation of new extracellular matrix. The extracellular matrix consists mainly of collagen but also fractions of elastin. It is thought that elastin is responsible for maintaining elastic properties of the myocardium, thus reducing the risk of premature rupture. An elastin\/tropoelastin\u2013specific contrast agent (Gd\u2010\n ESMA<\/jats:styled-content>\n ) was used to image tropoelastin and mature elastin fibers for in\u00a0vivo assessment of extracellular matrix remodeling post\u2010\n MI<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results<\/jats:title>\n \n Gd\u2010\n ESMA<\/jats:styled-content>\n enhancement was studied in a mouse model of myocardial infarction using a 7\u00a0T\n MRI<\/jats:styled-content>\n scanner and results were compared to those achieved after injection of a nonspecific control contrast agent, gadolinium\u2010diethylenetriamine pentaacetic acid (Gd\u2010\n DTPA<\/jats:styled-content>\n ). In the infarcted tissue, Gd\u2010\n ESMA<\/jats:styled-content>\n uptake (measured as R1 relaxation rate) steadily increased from day 3 to day 21 as a result of the synthesis of elastin\/tropoelastin. R1 values were in good agreement with histological findings. A\u00a0similar R1 behavior was observed in the remote myocardium. No mature cross\u2010linked elastin was found at any time point. In contrast, Gd\u2010\n DTPA<\/jats:styled-content>\n uptake was only observed in the infarct with no changes in R1 values between 3 and 21\u00a0days post\u2010\n MI<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n We demonstrate the feasibility of in\u00a0vivo imaging of extracellular matrix remodeling post\u2010\n MI<\/jats:styled-content>\n using a tropoelastin\/elastin binding\n MR<\/jats:styled-content>\n contrast agent, Gd\u2010\n ESMA<\/jats:styled-content>\n . We found that tropoelastin is the main contributor to the increased\n MRI<\/jats:styled-content>\n signal at late stages of\n MI<\/jats:styled-content>\n where its augmentation in areas of infarction was in good agreement with the R1 increase.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1161\/jaha.115.001851","type":"journal-article","created":{"date-parts":[[2015,8,14]],"date-time":"2015-08-14T01:54:32Z","timestamp":1439517272000},"update-policy":"https:\/\/doi.org\/10.1161\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":42,"title":["Assessment of Myocardial Remodeling Using an Elastin\/Tropoelastin Specific Agent with High Field Magnetic Resonance Imaging (MRI)"],"prefix":"10.1161","volume":"4","author":[{"given":"Andrea","family":"Protti","sequence":"first","affiliation":[{"name":"Cardiovascular Division, James Black Centre, King's College Hospital, London, United Kingdom"},{"name":"Division of Imaging Sciences and Biomedical Engineering, King's College London, King's Health Partners, St. Thomas\u2019 Hospital, London, United Kingdom"},{"name":"Cardiovascular Division, The British Heart Foundation Centre of Excellence, King's College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bego\u00f1a","family":"Lavin","sequence":"additional","affiliation":[{"name":"Division of Imaging Sciences and Biomedical Engineering, King's College London, King's Health Partners, St. Thomas\u2019 Hospital, London, United Kingdom"},{"name":"Cardiovascular Division, The British Heart Foundation Centre of Excellence, King's College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuebin","family":"Dong","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, James Black Centre, King's College Hospital, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Lorrio","sequence":"additional","affiliation":[{"name":"Division of Imaging Sciences and Biomedical Engineering, King's College London, King's Health Partners, St. Thomas\u2019 Hospital, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Robinson","sequence":"additional","affiliation":[{"name":"Lantheus Medical Imaging, North Billerica, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Onthank","sequence":"additional","affiliation":[{"name":"Lantheus Medical Imaging, North Billerica, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajay M.","family":"Shah","sequence":"additional","affiliation":[{"name":"Cardiovascular Division, James Black Centre, King's College Hospital, London, United Kingdom"},{"name":"Cardiovascular Division, The British Heart Foundation Centre of Excellence, King's College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene M.","family":"Botnar","sequence":"additional","affiliation":[{"name":"Division of Imaging Sciences and Biomedical Engineering, King's College London, King's Health Partners, St. Thomas\u2019 Hospital, London, United Kingdom"},{"name":"Cardiovascular Division, The British Heart Foundation Centre of Excellence, King's College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"276","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070885"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20111009"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1067\/mje.2002.117560"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmg.2007.11.007"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12410-012-9176-y"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2473070975"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-013-0397-2"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2009.07.015"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.115.14.2817"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/30522"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.2001.1491"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/415171a"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9149(73)80153-5"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb05868.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCIMAGING.113.001270"},{"issue":"9","key":"e_1_3_3_17_2","first-page":"I81","article-title":"Elastin stabilizes an infarct and preserves ventricular function","volume":"112","author":"Mizuno T","year":"2005","unstructured":"Mizuno T, Yau TM, Weisel RD, Kiani CG, Li RK. 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